Ageing-type saccharomyces cerevisiae and its use in wine making
A technology of Saccharomyces cerevisiae, NX11424, applied in the field of microorganisms, can solve the problems of the wine industry losing international competitiveness, reducing yeast diversity, and reducing the importance of yeast diversity, local yeast, etc., to achieve excellent color and aroma extraction ability, obvious The effect of aging potential, perfect bone and structure
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Embodiment 1
[0022] Example 1. Isolation, purification and identification of Saccharomyces cerevisiae NX11424 (preserved in the "General Microbiology Center of China Microbiological Culture Collection Management Committee", the preservation number is CGMCCNO.10050)
[0023] Pick the Cabernet Sauvignon grapes from the three-base vineyard in Guangxia, Ningxia, take the fermentation broth during the natural fermentation process, add 9 mL of sterile water to 1 mL of the fermentation liquid sample for gradient dilution, coat the WLN solid medium plate, and store it at a temperature of 28 °C Under culture 5d, according to the bacterium colony characteristic of Saccharomyces cerevisiae on WLN medium (refer to figure 1 ): Select a single colony with a smooth and creamy surface, a central conical protrusion, and a dark yellow or light green ring around it, opaque, and creamy, and pick a single colony of S. A single colony of Saccharomyces cerevisiae was picked from WLN medium and transferred into Y...
Embodiment 2
[0033] Example 2, comparative experiment of Saccharomyces cerevisiae NX11424 and commercial yeast F15 pilot-scale brewing
[0034] The technological process of following embodiment sees figure 2 .
[0035] Destemmed fresh Cabernet Sauvignon grapes collected from Ningxia Yuma, added 50mg / L sulfur dioxide, added 20mg / L pectinase after 30 minutes, and measured the total acid and total sugar content of the grape juice after mixing. Fresh grape juice was used to activate the strain, and the test strain was inserted into an appropriate amount of grape juice with a 3% inoculum. After cultivating in a constant temperature incubator at 28°C for 24 hours, the activated test strain was diluted with 10 6 The inoculum amount of cfu / mL was added to the fermenter, and the strains were set up in two parallels. Control the fermentation temperature at 22-25°C. After 3 days of fermentation, adjust the temperature again to 28-30°C for fermentation. Cap the fermenter three times a day in the mo...
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