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Vegetable protein/soybean polysaccharide nano-emulsion embedding natural fat-soluble pigment and preparation method of vegetable protein/soybean polysaccharide nano-emulsion

A soybean polysaccharide and nanoemulsion technology, which is applied in the fields of polysaccharide/gum-containing food ingredients, food science, and oil-containing food ingredients, etc., can solve the problems of no industrialized production, and achieve short production cycle, simple preparation process, and easy reaction process. control effect

Inactive Publication Date: 2016-02-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as we know, there is no report on the industrial production of protein / polysaccharide nanoemulsions embedded with lycopene, and the research on the embedding, protection and release of active substances is limited to scientific research

Method used

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  • Vegetable protein/soybean polysaccharide nano-emulsion embedding natural fat-soluble pigment and preparation method of vegetable protein/soybean polysaccharide nano-emulsion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Dissolve soybean acid-soluble protein and soluble soybean polysaccharide in deionized water respectively, stir for 2 hours, adjust the pH of the solution to 2.0 with a mixed solution of citric acid, malic acid and sodium citrate with a total acid concentration of 0.1mol / L, and obtain protein Storage solution and polysaccharide storage solution, protein storage solution is added in the polysaccharide storage solution and mixed uniformly to obtain a mixed solution, the weight-to-volume ratio of soybean acid-soluble protein in the control mixed solution is 0.5%, and the mass ratio of soybean acid-soluble protein to soybean polysaccharide is 1:1; then dissolve lycopene in corn oil, and add it together with mogroside into the above mixed solution, and control the amount of corn oil added to the mass volume ratio of the mixed solution to be 3g / 100ml, The mass-volume ratio of the amount added relative to the mixed solution is 0.05g / 100ml, stirred at 10000rpm for 1min, and then ...

Embodiment 2

[0033]Dissolve pea acid-soluble protein and soluble soybean polysaccharide in deionized water respectively, stir for 2.5 hours, adjust the pH of the solution to 3.0 with a mixed solution of citric acid, malic acid and sodium citrate with a total acid concentration of 0.1mol / L, and obtain Protein storage solution and polysaccharide storage solution, the protein storage solution is added to the polysaccharide storage solution and mixed evenly to obtain a mixed solution, the weight-to-volume ratio of pea acid-soluble protein in the mixed solution is controlled to be 0.5%, and the mass ratio of pea acid-soluble protein to soybean polysaccharide The ratio is 1:3; then dissolve lycopene in salad oil, and then add it together with mogroside into the above mixed solution. The mass volume ratio of the added amount relative to the mixed solution is 0.05g / 100ml, stirred at 10000rpm for 1min, and then used a high-pressure micro-jet nano-homogenizer to homogenize for 2min at a pressure of 5...

Embodiment 3

[0035] Dissolve kidney bean acid-soluble protein and soluble soybean polysaccharide in deionized water respectively, stir for 3 hours, adjust the pH of the solution to 4.0 with a mixed solution of citric acid, malic acid and sodium citrate with a total acid concentration of 0.1mol / L, and obtain protein Storage solution and polysaccharide storage solution, the protein storage solution is added in the polysaccharide storage solution and mixed evenly to obtain a mixed solution, the weight-to-volume ratio of the kidney bean acid-soluble protein in the control mixed solution is 0.5%, and the mass ratio of the kidney bean acid-soluble protein to soybean polysaccharide is 1:4; then dissolve lycopene in corn oil, add it together with mogroside into the above mixed solution, control the amount of corn oil added relative to the mass volume ratio of the mixed solution to be 3g / 100ml, the amount of lycopene The mass-volume ratio of the added amount relative to the mixed solution is 0.06g / 1...

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Abstract

The invention belongs to the technical field of food processing, and discloses a vegetable protein / soybean polysaccharide nano-emulsion embedded with a natural fat-soluble pigment and a preparation method of the vegetable protein / soybean polysaccharide nano-emulsion. The preparation method comprises the following steps: dissolving vegetable acid-soluble protein and soluble soybean polysaccharide into deionized water respectively, stirring for 2 to 3 hours, adjusting the pH values of the stirred solutions I to be 2.0 to 4.0 to obtain a protein stock solution and a polysaccharide stock solution respectively, adding the protein stock solution into the polysaccharide stock solution, mixing to obtain a uniform mixed solution II; dissolving the natural fat-soluble pigment into grease, adding the grease containing the dissolved natural fat-soluble pigment and mogroside into the mixed solution II, and carrying out stirring and homogenizing to obtain an emulsion I; heating the homogenized emulsion I at 80 to 90 DEG C for 0.5 to 1 hour, so as to obtain the vegetable protein / soybean polysaccharide nano-emulsion embedded with the natural fat-soluble pigment. The vegetable protein / soybean polysaccharide nano-emulsion is high in natural pigment content, low in sugar content, small in particle size distribution, and high in stability under an acidic condition, and can be used for development of functional foods and health products.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a plant protein / soybean polysaccharide nanoemulsion embedding a natural fat-soluble pigment and a preparation method. Background technique [0002] Nanoemulsion has anti-sedimentation and anti-emulsion kinetic stability, and is a good embedding and delivery carrier for fat-soluble active substances. Natural fat-soluble pigments such as lycopene are a kind of fat-soluble active substances with strong ability to scavenge free radicals. substance. Protein-stabilized emulsions are very sensitive to environmental stresses such as pH, ionic strength, and temperature. When the pH value in the emulsion is close to the isoelectric point of the protein and / or higher salt concentration, the electrostatic repulsion of the protein adsorption layer decreases, so coalescence and flocculation. The protein adsorption layer at the oil-water interface can prevent the droplets ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/00A23L29/00A23L29/30
CPCA23V2002/00A23V2200/02A23V2250/18A23V2250/254A23V2250/51A23V2250/5488A23V2250/213A23V2250/548A23V2250/2112
Inventor 齐军茹刘倩茹冯纪璐
Owner SOUTH CHINA UNIV OF TECH
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