Method for preparing porous artificial nerve conduit with orientation structures on inner walls

Abstract

The invention belongs to the field of medical biological materials capable of being implanted in human bodies, and relates to tissue engineering nerve graft for repairing injured nerves and a method for preparing the tissue engineering nerve graft. The tissue engineering nerve graft and the method have the advantages that micro-pattern techniques and freeze-dry molding processes are combined with one another, accordingly, an artificial nerve conduit favorable for adhesion and growth of nerve cells can be provided, orientation stripe structures are arranged on the inner walls of the conduit, the walls of the conduit are of loose porous structures, and suture or adhesion of the side walls of the conduit can be omitted during usage; the method for preparing the nerve conduit is simple and feasible and is applicable to mass production.

Description

technical field [0001] The invention belongs to the field of medical biomaterials that can be implanted into human bodies, and in particular relates to a tissue-engineered nerve guide for repairing nerve damage and a preparation method thereof. Background technique [0002] In my country, the number of patients with peripheral nerve injury is increasing year by year. Although peripheral nerves have a certain self-repair function, it is difficult to achieve complete self-repair (especially for long-distance nerve defects), so it needs to be repaired with the help of various nerve bridges. Although autologous scaffolds are the gold standard for repairing peripheral nerve defects, they face problems such as limited donor sources, mismatched sizes, and permanent damage to the harvested site. At present, people mainly rely on various tissue-engineered nerve bridges prepared from natural or synthetic biomaterials with good biocompatibility and biodegradability to achieve nerve re...

AI Technical Summary

Problems solved by technology

However, there are still some difficulties in the construction of the inner wall of the catheter with a specific spatial morphology (orientation)

Although the molding method is easy to prepare catheters, it is difficult to obtain catheters with oriented inner walls, mainly because of the difficulty of peeling

The electrospinning method can prepare oriented spun fiber membranes, and then curl the membranes to form a catheter with an oriented inner wall, but the side of the catheter needs to be closed by suturing or bonding, which will increase the risk of inflammation Probability; In addition, the spinning can be directly spun onto the cylindrical receiving device, but because the prepared catheter is closely combined with the mold, the inner wall of the catheter is easily damaged when peeling off

Micrographic technology can easily construct a two-dimensional biomaterial surface with orientation, and then curl the material into a catheter shape, but the side of the catheter also needs to be closed by suturing or bonding, which also increases the risk of inflammation. probability