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Solen active tripeptide and preparation method and application thereof

A clam clam and active technology, applied in the field of clam clam active tripeptide and its preparation, can solve the problems of different flavors and shapes, and achieve the effect of mild enzymatic hydrolysis, low cost and remarkable effect

Active Publication Date: 2016-02-10
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The shape of razor clam and razor clam is different, and the flavor is slightly different when eating. At present, the research on enzymatic polypeptide of razor clam has not been reported. It is of great significance to increase the economic value of clam clams with multi-basic functional foods and medicines

Method used

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  • Solen active tripeptide and preparation method and application thereof
  • Solen active tripeptide and preparation method and application thereof
  • Solen active tripeptide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Preparation of the active tripeptide of clams

[0031] 1. Pre-processing:

[0032] Take fresh razor clams, remove the shells and get the razor clams meat, wash them and drain them for later use.

[0033] 2. Alkaline proteolysis

[0034] 2.1 Use a high-speed tissue masher to mash the razor clam meat, add distilled water to homogenize, the ratio of material to liquid is 1:1 to 3, accurately weigh the homogenate, and use 0.1mol / L hydrochloric acid solution and 0.1mol / L NaOH solution. Adjust the pH value of the homogenate, add alkaline protease for enzymatic hydrolysis for several hours, the enzymatic hydrolysis conditions are: enzymatic hydrolysis temperature 35-50 ℃, pH 8-10.4, enzymatic hydrolysis time 2-8 h, adding enzyme amount 300-1200U / g bamboo clams Meat. After the enzymatic hydrolysis, the enzyme was inactivated at 100 °C for 15 min, centrifuged at 4 °C for 15 min (10000 r / min), and the supernatant was taken for later use.

[0035] 2.2 Alkaline prote...

Embodiment 2

[0061] Example 2: Study on the anti-PC-3 cell proliferation activity of bamboo clam active tripeptide

[0062] Taking the F12 medium as the control group, the above-mentioned MTT method was used to compare the effect of the active tripeptide of bamboo clams on the proliferation of PC-3 cells with different concentrations and different action times. The test results are as follows. Figure 4 As shown, it can be seen that the active tripeptide of razor clam can effectively inhibit the proliferation of PC-3 in a concentration- and time-dependent manner.

[0063] Main materials and reagents used in the above examples:

[0064] Bamboo clams were purchased from Nanzhen Market, Zhoushan City, Zhejiang Province; prostate cancer cells PC-3 hormone-independent cells (originally purchased from Shanghai Cell Bank of Chinese Academy of Sciences, passaged and preserved by our laboratory); fetal bovine serum (FBS), Hangzhou Sijiqing Biotechnology Products Co., Ltd.; HamF12 medium, Gibco com...

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Abstract

The invention relates to solen active tripeptide with the amino acid sequence of Pro-Gly-Asp, and ESI / MS detection shows that the molecular weight is 287.3 Da. The solen active tripeptide is prepared through the following steps of shelling fresh solen to take meat, washing and triturating solen meat, conducting tissue homogenate, regulating the pH value of the homogenate solution for standby application after homogenate is conducted, adding alkaline protease to the homogenate solution to conduct enzymolysis, obtaining an enzymolysis sample, conducting ultrafiltration and G-25 gel separation reversed high performance liquid chromatography purification on the obtained enzymolysis sample, collecting eluting peak components, conducting amino acid sequencing, and obtaining the solen active tripeptide. The maximum proliferation inhibition rate of the solen active tripeptide for prostate cancer cells PC-3 of human bodies can reach up to 90% or above, theoretical basis can be provided for research on prostate cancer prevention medicine, the solen active tripeptide is obtained through the biological enzymolysis method, raw materials are wide in source and low in cost, and great significance in further researching and developing medicine and food with solen as foundation and improving economic value of solen is achieved.

Description

technical field [0001] The invention relates to the field of biologically active peptides, in particular to an active tripeptide of bamboo clams and a preparation method and application thereof. Background technique [0002] Bamboo razor, also known as the king of razor clams, is a marine bivalve mollusk with an elongated body and a bamboo tube shape after the two shells are hugged together, hence the name of bamboo razor. Bamboo razor shells are smooth, yellow-brown, shiny, brittle and thin, with prominent surface, dorsal and ventral margins parallel, the rear end is pure round, rectangular, with obvious growth lines, about 11 cm long. It is distributed in the north and south coasts of my country. The meat is delicious and can be eaten fresh or dried. Bamboo razor meat is sweet and salty in taste, cold in nature, enters the heart, liver, and kidney meridians; it has the functions of nourishing yin, clearing heat, eliminating vexation, and detoxification; have a certain th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/097C12P21/06C07K1/34C07K1/20C07K1/16A61K38/06A61P35/00A23L1/29
Inventor 黄芳芳丁国芳杨最素余方苗
Owner ZHEJIANG OCEAN UNIV