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Electric potential type glucose recognition element and glucose detection method thereof

An identification element and potential type technology, which is applied in the field of glucose analysis and testing, can solve the problems of cumbersome operation and achieve the effect of improving selectivity, reducing design difficulty, and easy operation

Inactive Publication Date: 2016-02-10
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent decades, many boronic acid-based glucose sensors have been reported. In addition to using boronic acid groups as glucose recognition elements, these sensors also need another group to report the recognition process; at the same time, signal readout often requires complex optics. equipment, more cumbersome to operate

Method used

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  • Electric potential type glucose recognition element and glucose detection method thereof
  • Electric potential type glucose recognition element and glucose detection method thereof
  • Electric potential type glucose recognition element and glucose detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The present invention uses 4,4'-benzenediboronic acid as an example to detect glucose. The operation steps are as follows:

[0027] (1) Preparation of polymer membrane electrodes

[0028] Weigh 196mg of polyvinyl chloride, 196mg of o-nitrophenyloctyl ether, and 4.54mg of tridodecylmethylammonium chloride and dissolve them in 3.5mL of tetrahydrofuran. in the glass ring. After the tetrahydrofuran was volatilized, the membrane was cut into small discs with an inner diameter of 3 mm using a puncher and pasted on the tip of a pipette with a PVC tube at the bottom. Use 10mM NaCl solution as the inner filling solution and activation solution to activate the electrode, ready for use.

[0029] (2) Potential detection of glucose

[0030] 50 mM 4,4'-benzenediboronic acid was prepared in dimethyl sulfoxide, and then added to a PBS buffer solution with a pH of 7.4 so that the final concentration of 4,4'-benzenediboronic acid was 50 μM, and the potential value was recorded. Afte...

Embodiment 2

[0032] The present invention uses 4,4'-biphenyl diboronic acid as a probe to detect glucose as an example. The operation steps are as follows:

[0033] (1) Preparation of polymer membrane electrodes

[0034] Weigh 196mg of polyvinyl chloride, 196mg of o-nitrophenyloctyl ether, and 5.76mg of tetradodecyl ammonium chloride, dissolve them in 3.5mL of tetrahydrofuran, stir them evenly, and pour them into a glass ring with an inner diameter of 3.5cm fixed on a glass plate middle. After the tetrahydrofuran was volatilized, the membrane was cut into small discs with an inner diameter of 3 mm using a puncher and pasted on the tip of a pipette with a PVC tube at the bottom. Use 10mM NaCl solution as the inner filling solution and activation solution to activate the electrode, ready for use.

[0035] (2) Potential detection of glucose

[0036] Use dimethyl sulfoxide to prepare 50 mM 4,4'-biphenyl diboronic acid, and then add it to the PBS buffer solution with a pH of 7.4. Add the ab...

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Abstract

The invention belongs to the technical field of glucose analysis tests and particularly relates to a high-selectivity and non-enzyme electric potential type glucose detection method. The method specifically includes the steps that boric acid can cause anionic potential response on a polymer membrane electrode doped with quaternary ammonium salt, a boronic acid derivative containing double boric acid groups is utilized as a probe, after the probe and glucose generate an oligomerization function, further reduction of electric potential is caused, and other monosaccharides cannot generate an oligomer with the probe, so that obvious reduction of electric potential does not exist. By means of the high-selectivity and non-enzyme electric potential type glucose detection method, high-selectivity and non-enzyme electric potential detection can be achieved.

Description

technical field [0001] The invention belongs to the technical field of glucose analysis and testing, and in particular relates to a highly selective, enzyme-free potential type glucose recognition element and a method for detecting glucose. Background technique [0002] Sugar has important physiological significance in the life activities of the human body. Abnormal sugar content in the blood is closely related to metabolic disorders. Therefore, blood sugar level is often used as a sign of diseases such as diabetes, cardiovascular and cerebrovascular diseases, and obesity. At the same time, blood glucose is also a frequently detected item in postoperative clinical care, and a simple and easy detection method is very much needed. Traditional sugar detection methods such as blood glucose meters generally require the help of glucose oxidase. Although this type of sensor usually has good selectivity and sensitivity, due to the instability of glucose oxidase, it is very vulnerabl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 秦伟李龙
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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