Rapid extraction method of radix aconite lateralis preparata total alkaloids

A technology of total alkaloids and extraction method, which is applied in the field of rapid extraction of total alkaloids of aconite, can solve the problems of long extraction time, large amount of extraction liquid, and large amount of high-concentration organic solvents, etc., and achieves short extraction time and high extraction rate , The effect of low equipment requirements

Active Publication Date: 2016-02-17
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a quick extraction method for the total alkaloids of Aconitum aconitum (Aconitum aconitum) in view of the shortcomings of long extraction time, large amount of extracting liquid, and large amount of high-concentration organic solvents

Method used

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  • Rapid extraction method of radix aconite lateralis preparata total alkaloids
  • Rapid extraction method of radix aconite lateralis preparata total alkaloids

Examples

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Effect test

Embodiment 1

[0030] 1. Wash fresh aconite;

[0031] 2. Beat the fresh aconite with 4 times of 0.2% hydrochloric acid and separate the pulp and residue, and repeat the pulping once for the coarse residue to obtain residue and slurry;

[0032] 3. Separating the obtained slurry from water to solid-liquid to obtain starch and clear liquid;

[0033] 4. Wash the starch and slag with 0.5 times the weight of fresh aconite water twice and dehydrate to obtain the cleaning solution; dry the starch;

[0034] 5. After merging the part of the clear liquid obtained in 3 (30% of the clear liquid obtained in step 3) and the cleaning liquid obtained in step 4 to supplement the acid concentration (hydrochloric acid solution with a mass percentage concentration of 0.2%), return to 2 and reuse it as the extract ;

[0035] 6. Remove the protein from the remaining clear liquid, and adjust the pH to 8 by adding alkali;

[0036] 7. Put the pH-adjusted supernatant on a D101 macroporous resin column, elute with 5...

Embodiment 2

[0038] 1. Wash fresh aconite;

[0039] 2. Beat the fresh aconite with 8 times of 0.6% citric acid and separate the pulp and residue, and repeat the pulping once for the coarse residue to obtain residue and slurry;

[0040] 3. Separating the obtained slurry from water to solid-liquid to obtain starch and clear liquid;

[0041] 4. Wash the starch and slag with fresh water of 1 times the weight of fresh aconite and dehydrate to obtain a cleaning solution; dry the starch;

[0042] 5. Combine the part of the clear liquid obtained in 3 (60% of the clear liquid obtained in step 3) and the cleaning liquid obtained in step 4 to supplement the acid concentration (citric acid solution with a concentration of 0.6% by mass) and return to step 2 as an extract reuse;

[0043] 6. Remove the protein from the remaining clear liquid, and adjust the pH to 8 by adding alkali;

[0044] 7. Put the pH-adjusted supernatant on a D101 macroporous resin column, elute with 5BV80% ethanol after removing...

Embodiment 3

[0046] 1. Wash fresh aconite;

[0047] 2. Beat the fresh aconite with 1 times 0.5% acetic acid and separate the pulp and slag, and repeat the beating once for the coarse slag to obtain slag and pulp water;

[0048] 3. Separating the obtained slurry from water to solid-liquid to obtain starch and clear liquid;

[0049] 4. Wash the starch and slag twice with fresh water of 1 times the weight of fresh aconite and dehydrate to obtain the cleaning solution; dry the starch;

[0050] 5. Combine the part of the clear liquid obtained in 3 and the cleaning liquid obtained in step 4 to supplement the acid concentration (0.5% acetic acid solution in mass percent concentration) and then return to 2 for reuse as the extract;

[0051] 6. Deproteinize the remaining supernatant, add alkali to adjust the pH to 9;

[0052] 7. Put the pH-adjusted supernatant on a D101 macroporous resin column, elute with 5BV80% ethanol after removing impurities with distilled water, and obtain the total alkaloi...

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Abstract

The invention provides a rapid extraction method of radix aconite lateralis preparata total alkaloids. The method comprises the steps that 1, pulping is conducted on cleaned fresh radix aconite lateralis preparata through a total alkaloid extracting solution, slurry-residue separation is conducted, pulping is repeatedly conducted on coarse residues, and residues and slurry are obtained; 2, solid-liquid separation is conducted on the slurry obtained in the first step, and starch and clear liquid are obtained; 3, the starch obtained in the second step and the residues obtained in the first step are cleaned through clean water for multiple times, dehydration is conducted, and cleaning fluid is obtained; 4, part clear liquid obtained through separation in the second step and the cleaning fluid obtained in the third step are combined and serve as the total alkaloid extracting solution for reutilization by returning to the first step; 5, protein is removed for the residual obtained clear liquid, and pH is regulated; 6, the clear liquid regulating the pH in the fifth step is used for a macroporous resin column, purification is conducted through distilled water, elution is conducted through ethyl alcohol, and after solvent is distilled through eluant, the radix aconite lateralis preparata total alkaloids are obtained. According the rapid extraction method of the radix aconite lateralis preparata total alkaloids, dosage of the extracting solution is saved, and the extracting time is shortened.

Description

technical field [0001] The invention relates to the technical field of medicine preparation, in particular to a method for rapidly extracting total alkaloids from aconite. Background technique [0002] The main active ingredient of aconitum plants is total alkaloids of aconitum, which has strong sedative, analgesic, cardiotonic, anti-inflammatory and anti-tumor effects. The diester alkaloids are very toxic, and the clinically toxic amount It is very close to the treatment amount, so many studies focus on the detoxification of aconitum alkaloids. At present, how to rationally use the total alkaloids to develop new drugs has been paid attention to by many researchers, such as the total aconitine patch, aconitum injection, etc., and the development of biopesticides using the toxicity of the total aconitum has also made great achievements. Good effect, also has a certain application in drug rehabilitation. [0003] The conventional method of extracting total alkaloids of Aconi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/714
Inventor 曾荣
Owner SOUTHWEAT UNIV OF SCI & TECH
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