Unlock instant, AI-driven research and patent intelligence for your innovation.
A method for rapid identification of the species of pickled cotton stings
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A pickling and rapid technology, which is applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., to achieve the effect of high accuracy, less time-consuming, and less sample volume
Active Publication Date: 2019-02-12
DALIAN POLYTECHNIC UNIVERSITY
View PDF2 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
But so far no one has applied this method to the identification of pickled jellyfish species
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0053] 1. Desalinize the pickled cotton stings, change the water once every 12 hours, and change 4 times in total, use ethanol solution as the stock solution, store the dehydrated cotton stings in a solution with a final concentration of 70% ethanol (v / v), and store ;
[0054] 2. Take out 150 mg of the jellyfish stings stored in the ethanol solution, cut them into pieces, centrifuge at 10,000 rpm for 5 minutes, put them on a dust-free paper to absorb the water, and use the deep-processed food DNA extraction kit to extract jellyfish genomic DNA;
[0055] 3. Use 16S rRNA gene universal primers for PCR amplification, and store the products at -20°C;
[0056] 4. The PCR amplified product was digested with Hind III, Hpa I, Dra I and Xho I endonucleases, and the digested products were separated by 3% agarosegel electrophoresis and detected by imaging.
[0057] The result is as Figure 5 Shown, wherein, A represents Hind III, B represents Hpa I, C represents Xho I, and D represent...
Embodiment 2
[0060] 1. Purchase 4 kinds of commercially available pickled jellyfish from Shandong and Liaoning, numbered 1, 2, 3, 4, respectively, and carry out desalination treatment, change the water once every 12 hours, and change the water 4 times in total. Use ethanol solution as the stock solution, and dehydrate the jellyfish. The jellyfish is stored in a solution with a final concentration of 70% ethanol (v / v), for future use;
[0061] Among them, 1 represents the pickled jellyfish purchased from Yantai, Shandong, 2 represents the pickled jellyfish purchased from Yingkou, Liaoning, and 3 and 4 both represent the pickled jellyfish purchased from Dalian, Liaoning.
[0062] 2. Take out 150 mg of jellyfish stored in ethanol solution, cut it into pieces, centrifuge at 7500rpm for 10 minutes, put it on a dust-free paper to absorb the water, and use the deep-processed food DNA extraction kit to extract jellyfish genomic DNA;
[0063] 3. Use 16S rRNA gene universal primers for PCR amplifica...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses a method for rapidly identifying species of salted rhopilema esculentum kishinouye. The method comprises steps as follows: preprocessing rhopilema esculentum kishinouye; extracting DNA (deoxyribonucleic acid); adopting rhopilema16S rRNA gene universal primers for PCR (polymerasechain reaction) amplification; performing RFLP (restriction fragment length polymorphism) analysis by the aid of restriction enzyme. The method has the characteristics of simplicity and high efficiency, the detection is convenient and is not time-consuming, the species of the commercially available salted rhopilema esculentum kishinouye can be rapidly determined, and the customer's benefit is guaranteed.
Description
technical field [0001] The invention relates to a method for identifying the species of pickled jellyfish in the market, and more specifically, relates to a rapid identification method for the species of pickled jellyfish. Background technique [0002] The cotton stinger (Rhopilema esculentum Kishinouye) is an important breed of jellyfish in northern my country. The jellyfish near the Liaodong Peninsula are mainly divided into cotton stinger and sand stinger (Stomolophus meleagris). Sand stings are low-grade products, while cotton stings are high-quality products of jellyfish. Usually, the market prices of the two kinds of jellyfish are: sand stings 3-5 yuan / catties, cotton stings 8-10 yuan / catties. The identification of cotton stingers and sand jellyfish is currently mainly focused on morphology, but after processing, it is not suitable for product identification in terms of morphology, so it is difficult for consumers to distinguish species. The phenomenon of filling is ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More