Separation and purification method of verbascoside
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A technology for acteoside and crude acteoside, applied in the field of biology, can solve the problems of inconvenient operation, complex process, low efficiency, etc., and achieve the effects of optimized synthesis steps, simple process route, and convenient operation
Pending Publication Date: 2022-04-05
TAIZHOU DANDING BIOTECH CO LTD
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[0004] Acteoside is a phenylethanol glycoside component. Modern research has proved that the phenylethanol glycoside component is closely related to the pharmacological effects of Rehmannia glutinosa. The current extract of acteoside is mainly processed by heating extraction, membrane separation, resin purification, biotransformation, and chemical catalysis. , these methods have disadvantages such as complicated process, inconvenient operation, long cycle and low efficiency.
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Embodiment 1
[0040] The present invention provides a separation and purification method of acteoside, which is separated and extracted from the traditional Chinese medicine Rehmannia glutinosa. The separation method of acteoside comprises the following steps:
[0041] Step 1, preparing materials and reagents, including: 80% ethanol, adsorption resin, and ethanol aqueous solution with a volume concentration of 5% to 25%;
[0042] Step 2. Sample processing:
[0043] A. Pretreat the raw rehmannia medicinal material, cut it into small pieces and place it in the extraction tank;
[0044] B. Steam through the bottom of the tank for 40 minutes, and use saturated steam to boil the small pieces of rehmannia glutinosa;
[0045] C. Extract with ethanol, the extract is concentrated to remove ethanol, and the concentrate is diluted with water;
[0046] D. Combine the diluted extracts and filter to obtain a filtrate.
[0047] The separation and purification of verbascoside in step 3 also includes the...
Embodiment 2
[0060] On the basis of Example 1, the present invention provides a technical solution: preferably, the macroporous resin model is selected from: D101 resin, AB-8 macroporous adsorption resin, HPD100 resin, HPD-200 resin, HPD-200A resin, One or more combinations of HPD-400 resins, the gradient elution procedure is as follows: 5%, 10%, 20%, 25% volume concentration of ethanol aqueous solution for elution, ethanol extraction in c is reflux extraction or Ultrasonic extraction, the number of extractions is 2-5 times, each extraction time is 2-5 hours, the amount of ethanol added to each extraction is 1.5-12 times the weight of the crude drug, and the ethanol concentration is 0%-95%.
[0061] Beneficial effects: the acteoside product of the present invention is derived from the Scrophulariaceae plant, expands the plant sources of acteoside, has rich drug sources, optimizes and improves the extraction and preparation method of acteoside, is easy to operate and low in cost, and only us...
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Abstract
The invention discloses a method for separating and purifying verbascoside, and relates to the technical field of biology, the method is separated and extracted from traditional Chinese medicine rehmannia glutinosa, and the method for separating verbascoside comprises the following steps: preparing materials and reagents; performing sample treatment; and separating and purifying the verbascoside. According to the method, beta-glucosidase which is high in price and difficult in source is not used as an enzymolysis reagent, the process route is simpler, industrial production is easier to realize, the content of verbascoside prepared by the method is higher than that of verbascoside prepared by the prior art, the requirement of new drug application on the content can be met, and the method is suitable for industrial production. The verbascoside product is derived from scrophulariaceae plants, the plant source of the verbascoside is expanded, the medicine source is rich, the extraction and preparation method of the verbascoside is optimized and improved, the operation is simple and convenient, the cost is low, only ethanol is used as an organic reagent during operation, toxic reagents such as methanol and acetone are not used, and the verbascoside product is green and environment-friendly.
Description
technical field [0001] The invention relates to the technical field of biology, in particular to a method for separating and purifying verbascoside. Background technique [0002] Rehmannia glutinosa (scientific name: Rehmannia glutinosa) is the fresh or dried root tuber of Rehmannia glutinosa, a plant of the family Scrophulariaceae. Widely distributed in Henan, Hubei, Shanxi, Sichuan, Zhejiang, Inner Mongolia, Hunan, Shandong, Liaoning and other provinces and cities in China. Fresh Rehmannia glutinosa is cold in nature, sweet and bitter in taste, and has the effects of clearing heat and promoting body fluid, cooling blood, and stopping bleeding; raw rehmannia glutinosa is cold in nature, sweet in taste, has the effects of clearing heat and cooling blood, nourishing yin, and promoting body fluid; rehmannia glutinosa is slightly warm in nature, sweet in taste, and has the effect of nourishing yin and nourishing blood. , The effect of benefiting the essence and filling the mar...
Claims
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