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Leuconostoc mesenteroides as well as preparation method and application thereof

A technology for Leuconostoc membranaceus and seeds, which is applied in the field of microorganisms, can solve the problems of not producing insoluble polysaccharides, low yield of insoluble polysaccharides, etc., and achieves the effects of large amount of insoluble polysaccharides, simple and easy-to-operate cultivation methods, and broad market.

Inactive Publication Date: 2016-02-24
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is, aiming at the current situation that the existing Leuconostoc mesenteroides mainly produces soluble polysaccharides and does not produce insoluble polysaccharides or the yield of insoluble polysaccharides is very low, the present invention provides a strain of Leuconostoc mesenteroides ) and its cultivation method and application

Method used

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  • Leuconostoc mesenteroides as well as preparation method and application thereof
  • Leuconostoc mesenteroides as well as preparation method and application thereof
  • Leuconostoc mesenteroides as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The isolation of embodiment 1 bacterial strain BD3749

[0027] Take 1g of pickled pickled vegetables from farmers in Heilongjiang Province in a sterile manner, immerse them in sterile physiological saline, take the leachate for gradient dilution, spread the diluted solution evenly on solid TYC medium by coating, and culture at 30°C 24-48h. Multiple single colonies with sticky nose and good stringiness were selected and transferred to new solid TYC medium to obtain multiple purified colonies, and one of the single colonies was named BD3749.

Embodiment 2

[0028] Characteristics of Example 2 Bacterial Strain BD3749

[0029] 1. Colony characteristics

[0030] Take a single colony of strain BD3749, transfer it to TYC solid medium (agar), cultivate it in an anaerobic incubator at 37°C for 24 hours, and observe the size, color, edge, protrusion, smoothness, and viscosity of the colony , transparency and other characteristics. The result is as figure 1 shown. The results showed that the strain BD3749 formed mucus-like, well-drawn, neatly edged, raised, smooth, colorless and opaque single colonies.

[0031] 2. Morphological observation and physiological and biochemical characteristics

[0032] The fresh culture cultured on TYC solid medium (agar) for 24 hours was picked for physiological and biochemical tests. The results showed that BD3749 was a Gram-positive coccus, facultatively anaerobic. Contact enzyme test negative, oxidase negative.

[0033] 3. Identification characteristics of API50CH

[0034] Using the API50CH identi...

Embodiment 3

[0051] Example 3 Application of Leuconostoc mesenteroides BD3749

[0052] (1) Leuconostocmesenteroides (Leuconostocmesenteroides) BD3749 fermentation and preparation of fermentation broth

[0053] Preparation of lyophilized powder of Leuconostoc enterococcus BD3749: Pick 2 colonies, inoculate them in 300ml of 10% (w / w) skim milk solution, incubate at 30°C for 4h, pre-freeze overnight at -80°C, freeze at -20°C dry.

[0054] Dissolve 100 mg of lyophilized powder of Leuconostoc enterococci BD3749 in 0.1 ml of sterile distilled water, pick a ring with an inoculation loop and streak it on the TYC solid medium, culture it aerobically at 30°C for 24 hours, and then take it out.

[0055] Pick a single colony from the TYC solid medium, transfer it to 20ml liquid TYC medium, and culture it aerobically at 30°C for 24 hours to obtain the seed solution. The seed solution was transferred to a new liquid TYC culture solution at a ratio of 1% (v / v) of the inoculum amount, and aerobic static...

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Abstract

The invention discloses Leuconostoc mesenteroides as well as a preparation method and an application of the Leuconostoc mesenteroides in polysaccharide production. The Leuconostoc mesenteroides is collected in CGMCC (China General Microbiological Culture Collection Center), the collection number is CGMCC No. 10064, and the culture name is BD3749. The Leuconostoc mesenteroides can be used for large-scale preparation of insoluble polysaccharides, so that whey separation can be controlled better, the Leuconostoc mesenteroides can be applied to fermentation of dairy products, and influence on the quality and shelf life of the dairy products due to severe damage to texture of the products because of whey separation during storage of the dairy products can be avoided. A strain culture method is simple and easy to operate, large quantity of insoluble polysaccharides is prepared from the Leuconostoc mesenteroides, and the Leuconostoc mesenteroides is suitable for industrialized application and has broad market.

Description

technical field [0001] The invention relates to the field of microbes, in particular to Leuconostoc enterococci and its preparation method and application. Background technique [0002] Leuconostoc enterolis is an important species of Leuconostoc in lactic acid bacteria. U.S. FDA and AAFCO listed it as one of 42 kinds of safe microorganisms that can be directly eaten (feeding) in 1989. In 2012, the Ministry of Health of my country included Leuconostoc enterococci subspecies in the "List of strains that can be used in food". [0003] Studies have confirmed that Leuconostoc enterica not only has the high acid-producing ability, anti-oxidation ability and anti-pathogen characteristics of common lactic acid bacteria, but also has the ability to produce a variety of exopolysaccharides in large quantities during fermentation. Leuconostoc enteritidis has also been found widely in fermented foods, especially fermented dairy products, pickled vegetables and brewed fruit wine. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/12C12R1/01
CPCA23C9/1203C12N1/20C12N1/205C12R2001/01
Inventor 高彩霞吴正钧鄢明辉刘振民韩瑨徐晓芬
Owner BRIGHT DAIRY & FOOD
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