Application of chicken liver cancer cell system as duck plague virus host

Abstract

The invention belongs to the technical field of duck plague virus culturing, particularly relates to a new host cell of the duck plague virus, and discloses application of a chicken liver cancer cell system as a duck plague virus host. A traditional method for culturing the duck plague virus through the duck embryo fibroblast primary cell is limited by duck embryo supply and the hatching day age, and wastes time and labor during preparation. The established method for culturing the duck plague virus chicken liver cancer cell system is good in virus growth. The method for culturing the virus through a continuous cell line is not influenced by duck embryo supply, and has the advantages of being instantly available and capable of saving time and labor. The duck plague virus can grow and reproduce on a chicken liver cancer cell system continuous cell culture medium, and by means of a newly-found virus copying host cell, a new technical approach, method and material are provided for research of related fields.

Description

technical field [0001] The invention belongs to the technical field of duck plague virus cultivation, and in particular relates to a new host cell of duck plague virus. Background technique [0002] Duck plague (Duck Plague, DP) is caused by duck plague virus (Duck Plague Virus, DPV) and is common in ducks, geese, swans, geese and other waterfowl of Anseridae, an acute septic, highly contact and highly lethal infectious disease. The disease is widely prevalent, spreads rapidly, and has high morbidity and mortality. It is currently one of the most serious diseases to the waterfowl breeding industry in the world, and it is the enemy of the duck industry. The disease is widely distributed in our country and all over the world, and has caused serious harm, so the research on the disease is of great significance. [0003] DPV belongs to duck herpesvirus type 1 in the subfamily Herpesviridae. DPV can grow and reproduce in the chorioallantoic cavity of duck embryos at 9-14 days o...

AI Technical Summary

Problems solved by technology

The disadvantages of this method are as follows: ① limited by the supply of duck embryos, there are no fertilized eggs available for use during the rest period; ② affected by the source of duck embryos, there are some farms with poor feeding management and sanitary conditions, some bacteria The existence of pathogens such as Salmonella, Mycoplasma and other vertically transmitted diseases can easily lead to the contamination of the batch of DEF primary cells, which directly affects the subsequent experiments.

③The time period required for the traditional method to prepare cells is long, and the same process needs to be repeated for each preparation of DEF, and due to different batches of eggs, the biological characteristics of each preparation of DEF may be different

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