Application of chicken liver cancer cell system as duck plague virus host

A chicken liver cancer cell line and duck plague virus technology, applied in the field of new host cells of duck plague virus, can solve the problems of influence, DEF primary cell contamination, influence of duck embryo source, etc., and achieve the effect of good virus growth

Active Publication Date: 2016-03-23
JINLING INST OF TECH
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Problems solved by technology

The disadvantages of this method are as follows: ① limited by the supply of duck embryos, there are no fertilized eggs available for use during the rest period; ② affected by the source of duck embryos, there are some farms with poor feeding management and sanitary conditions, some bacteria The existence of pathogens such as Salmonella, Mycoplasma and other vertically transmitted dise

Method used

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  • Application of chicken liver cancer cell system as duck plague virus host
  • Application of chicken liver cancer cell system as duck plague virus host

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Embodiment 1

[0026] Cultivate LMH cells according to routine laboratory methods. When the cell density reaches about 95%, take the LMH that has just grown into a monolayer, discard the growth nutrient solution, wash the cell surface with sterilized PBS for 3 times, and then inoculate the DPV seed Added to the surface of LMH cells for inoculation. The duck plague virus liquid covers the cell surface for adsorption, and gently shakes the cell bottle every 5 minutes during the incubation to make the virus evenly distributed on the cells and fully infect. After adsorption at 37°C for 30 minutes, the virus solution was discarded, and then DMEM containing 3% calf serum, 100IU / ml penicillin and 100IU / ml streptomycin was added to maintain the nutrient solution at 37°C, and the changes of CPE were observed under an inverted microscope every day. figure 1 It is a schematic diagram of electron microscope ultrathin section of duck plague virus growing on chicken liver cancer cell line, and the arrow i...

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Abstract

The invention belongs to the technical field of duck plague virus culturing, particularly relates to a new host cell of the duck plague virus, and discloses application of a chicken liver cancer cell system as a duck plague virus host. A traditional method for culturing the duck plague virus through the duck embryo fibroblast primary cell is limited by duck embryo supply and the hatching day age, and wastes time and labor during preparation. The established method for culturing the duck plague virus chicken liver cancer cell system is good in virus growth. The method for culturing the virus through a continuous cell line is not influenced by duck embryo supply, and has the advantages of being instantly available and capable of saving time and labor. The duck plague virus can grow and reproduce on a chicken liver cancer cell system continuous cell culture medium, and by means of a newly-found virus copying host cell, a new technical approach, method and material are provided for research of related fields.

Description

technical field [0001] The invention belongs to the technical field of duck plague virus cultivation, and in particular relates to a new host cell of duck plague virus. Background technique [0002] Duck plague (Duck Plague, DP) is caused by duck plague virus (Duck Plague Virus, DPV) and is common in ducks, geese, swans, geese and other waterfowl of Anseridae, an acute septic, highly contact and highly lethal infectious disease. The disease is widely prevalent, spreads rapidly, and has high morbidity and mortality. It is currently one of the most serious diseases to the waterfowl breeding industry in the world, and it is the enemy of the duck industry. The disease is widely distributed in our country and all over the world, and has caused serious harm, so the research on the disease is of great significance. [0003] DPV belongs to duck herpesvirus type 1 in the subfamily Herpesviridae. DPV can grow and reproduce in the chorioallantoic cavity of duck embryos at 9-14 days o...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12R1/93
CPCC12N7/00C12N2710/16351
Inventor 郭宇飞晏文梅方光远杜改梅蒋加进罗碧平
Owner JINLING INST OF TECH
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