Application of adamts-2 gene and its expression product in diagnosis and treatment of gastric cancer

A technology for expressing products and genes, applied in the field of tumor markers, to achieve the effect of good application prospects, good stability and rapid response

Inactive Publication Date: 2018-07-06
张军利 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, differences in different experimental platforms and samples lead to many inconsistencies in the analysis results of each gene chip. Integrated analysis can collect and statistically integrate the results of related research reports published on the same issue, in order to obtain more accurate or more the result of

Method used

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  • Application of adamts-2 gene and its expression product in diagnosis and treatment of gastric cancer
  • Application of adamts-2 gene and its expression product in diagnosis and treatment of gastric cancer
  • Application of adamts-2 gene and its expression product in diagnosis and treatment of gastric cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Screening and analysis of GEO data

[0035] Restricting the study type to "expression profiling by array" datasets meeting the following criteria will be included in our study:

[0036] ①The selected data set must be the expression profile sequencing data of the whole genome;

[0037] ② These data come from the samples of gastric cancer case group and / or control group (without drug stimulation or transfection);

[0038] ③ This study considers standardized or original data sets.

[0039] Finally, 6 sets of microarray datasets were included in our study. The results are detailed in Table 1.

[0040] Table 1 Basic information of gastric cancer dataset

[0041]

[0042] All datasets were integrated to get 17481 genes. The probe IDs were converted to gene IDs according to the array annotations, the common genes of all expression profiles were screened, the missing data was filled for each set of expression profiles, and the data were normalized. Differenti...

Embodiment 2

[0043] The collection of embodiment 2 cases

[0044] All tissue samples were from gastric cancer patients diagnosed in the hospital from November 2012 to June 2014. When collecting materials, the central tissue of the cancer and the normal gastric mucosa tissue adjacent to the cancer were taken separately, and the specimens were quickly put into a small liquid nitrogen tank for preservation.

Embodiment 3

[0045] Example 3 extracts the total RNA in the sample

[0046] 1) Place the small pieces of sample tissue in a petri dish, add 1ml Trizol, cut it into pieces and move it to a homogenizer for homogenization;

[0047] 2) After the homogenization is completed, take out the homogenate and place it in an EP tube, set the volume to 1ml, and store it at -70°C;

[0048] 3) Place at 15-30°C for 5 minutes, add 200 μl chloroform, shake vigorously for 15 sec, and place at 15-30°C for 10 minutes;

[0049] 4) 2-8°C, centrifuge at less than 12000g / min for 10min, discard the supernatant, add 1ml of 75% ethanol, centrifuge at less than 7500g / min for 5min, discard the supernatant, and spot off;

[0050] 5) After the precipitate is dried (translucent, do not completely dry, otherwise the stability will be reduced), dissolve with RNase-freewater at 55-60°C, and freeze at -70°C.

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Abstract

The invention relates to an ADAMTS-2 gene and application of an expression product of the ADAMTS-2 gene in the diagnosis and treatment of stomach cancer. An inventor integrates the existing gene expression microarray data, six sets of stomach cancer expression data are obtained by retrieving and screening a GEO database, data are processed and analyzed again by utilizing a biological information analysis tool, the ADAMTS-2 gene is screened out, and validation of molecular biology is further performed, an RT-PCR result shows that high expression of the ADAMTS-2 gene is performed in gastric cancer tissues, an interference test indicates that ADAMTS-2-siRNA1 can effectively reduce the expression of the ADAMTS-2 gene. The invention provides a new target spot of diagnosis and treatment of stomach cancer, and has important clinical application value.

Description

technical field [0001] The invention relates to tumor markers, in particular to the application of ADAMTS-2 gene and its expression product in diagnosis and treatment of gastric cancer. Background technique [0002] Gastric cancer is a common malignant tumor of the digestive tract. In the world, the incidence of gastric cancer ranks fourth, and the mortality rate ranks second, second only to lung cancer. my country is a country with a high incidence of gastric cancer, and gastric cancer has shown a younger trend. At present, the main ways to treat gastric cancer are surgery, chemotherapy and radiotherapy. Although with the development of medical technology, the treatment effect of early gastric cancer has been greatly improved, but the survival rate of patients with advanced gastric cancer is still very low, the 5-year survival rate is less than 30%. leading cause of death. It is imminent to seek newer treatment methods for gastric cancer, especially gene-targeted therapy....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886G01N33/573A61K31/713A61P35/00
CPCA61K31/713C12Q1/6886C12Q2600/158G01N33/57446
Inventor 张军利漆楠楠张勇程岳雷
Owner 张军利
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