Composition of lotus flavones

A technology of lotus leaf flavonoids and composition, which is applied in the field of natural medicines and can solve problems such as separation and purification processes that have not been seen

Inactive Publication Date: 2016-03-30
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are no related patents and literature reports for the separation and purification process of quercetin-3-O-β-D-glucuronide in lotus leaves

Method used

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  • Composition of lotus flavones
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  • Composition of lotus flavones

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] The method steps of adopting macroporous adsorption resin to separate and purify a kind of lotus leaf flavonoid involved in the present invention are as follows:

[0100] (1) drying and pulverizing the lotus leaf to obtain the lotus leaf meal;

[0101] (2) Take by weighing 300g lotus leaf coarse powder, adopt 1: 30 (W / V) solid-liquid ratio to add 9 liters of 50% ethanol solution in this lotus leaf powder, 90 ℃ of hot reflux extraction time 3h, repeat leaching 3h The second time, the extracts were combined and then concentrated and dried to obtain the extract. The flavonoid weight content in the extract was 3%; the above extract was ultrasonically dissolved in distilled water and then dynamically loaded with macroporous adsorption resin LX-60 for purification;

[0102] (3) Use 10 times the column bed volume of distilled water and 25% ethanol to elute, and fully wash away a large amount of water-soluble impurities such as polysaccharides and proteins;

[0103] (4) Elute ...

Embodiment 2

[0107] (1) drying and pulverizing the lotus leaf to obtain the lotus leaf meal;

[0108] (2) Take 200g of lotus leaf coarse powder, add 8 liters of 70% ethanol solution to the lotus leaf coarse powder with a solid-liquid ratio (W / V) of 1:40, heat reflux at 80°C for 4 hours, and repeat the extraction 2 times, the extracts are combined and then concentrated and dried to obtain the extract, the flavonoid weight content in the extract is 3%. The above extracts were ultrasonically dissolved in distilled water and then purified by dynamic loading of macroporous adsorption resin LX-5;

[0109] (3) Elute with 8 times the column bed volume of distilled water and 18% ethanol to fully wash away a large amount of water-soluble impurities such as polysaccharides and proteins;

[0110] (4) Elute the macroporous resin with 30% ethanol;

[0111] (5) The 30% ethanol eluate was concentrated, freeze-dried, pulverized, and mixed uniformly to obtain a powder, and the weight content of quercetin-...

Embodiment 3

[0114] (1) lotus leaf is dried and pulverized to form lotus leaf meal;

[0115] (2) Take 400g of lotus leaf coarse powder, add 8 liters of 60% ethanol solution to the lotus leaf coarse powder with a material-to-liquid ratio (W / V) of 1:20, heat reflux at 70°C for 2 hours, and repeat the extraction 4 times, the extracts were combined, concentrated and dried to obtain the extract, and the flavonoid weight content in the extract was 4%. The above extracts were ultrasonically dissolved in distilled water and then purified by dynamic loading of macroporous adsorption resin AB-8;

[0116] (3) elution with 9 times column bed volume of distilled water and 20% ethanol to fully wash away a large amount of water-soluble impurities such as polysaccharides and proteins;

[0117] (4) adopt 32% ethanol to elute the macroporous resin;

[0118] (5) The 32% ethanol eluate was concentrated, dried under vacuum and reduced pressure, pulverized, and uniformly mixed to obtain a powder. The weight c...

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Abstract

The invention discloses a composition of lotus flavones. The composition is prepared by the following steps: adding an ethanol solution to crude powder of lotus leaves; dissolving an extract with distilled water and purifying the solution with macroporous resin to obtain flavone powder; and treating the powder by a high-performance liquid chromatograph to obtain the composition of the lotus flavones with the content of 90%-98%. The composition is novel in conception, simple in process and low in production cost; the obtained compound is high in purity; and industrial production is easy to achieve.

Description

technical field [0001] The invention relates to the field of natural medicines, in particular to a composition and a preparation method of lotus leaf flavonoids. Background technique [0002] Lotus leaf is the blade of Nymphaeaceae plant lotus (Nelumbonucifera 6aertn.), which is mainly produced in Jiangsu, Zhejiang, Hunan, Hubei, Anhui and other places. It is very rich in resources and has a huge output. In addition to conventional chemical components such as carbohydrates, lipids, proteins, and tannins, lotus leaves mainly contain three major types of compounds: alkaloids, polyphenols, and flavonoids. It is widely used in edible and medicinal fields. Because lotus leaf has good anti-oxidation, fat-removing weight loss, blood pressure lowering, antibacterial and other effects, and it has a wide range of sources and low price, it is one of the most valuable plants for development. However, the utilization rate of lotus leaf plant resources is very low at present, and only ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/62C07D311/30C07D311/40C07H17/07C07H1/08
Inventor 齐炼文孙晓月李飞范勇杨乐乐
Owner CHINA PHARM UNIV
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