Polyvinyl alcohol immobilized microorganism gel beads and preparation method and use thereof

A technology for immobilizing microorganisms and polyvinyl alcohol, applied in the field of environmental engineering, can solve the problems of severe polymer network formation conditions and large damage to microbial cells, and achieve the effects of improved mechanical properties, long duration and good mass transfer performance.

Inactive Publication Date: 2016-03-30
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Organically synthesized polymer compounds, such as polyvinyl alcohol (PVA), polyacrylamide (ACAM) and photochemical resins, have the advantages of anti

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Weigh 6g of polyvinyl alcohol (PVA) with a degree of polymerization of 1700-1800, add 0.25g of sodium alginate (SA) into 100mL of distilled water and mix, heat in a water bath to 90°C, and keep stirring with a glass rod until it is completely dissolved (no visible tiny particles), and keep warm for 5h to form a mixed gel solution of PVA and SA, and the final percentages of PVA and SA are 6% and 0.25%;

[0036] (2) After the PVA and SA mixed gel solution is cooled to room temperature, the activated sludge sludge water mixture is centrifuged to obtain wet bacteria, and the concentrated bacteria suspension is prepared in step (1) according to the ratio of 1:2 of the bacteria content. The mixed gel solution is mixed to obtain a mixed bacterial solution;

[0037] (3) Weigh 6g of boric acid (room temperature 25°C) and 2g of calcium chloride and dissolve them in 100mL of distilled water to prepare a crosslinking agent solution;

[0038] (4) Use a 10mL syringe to drop the ...

Embodiment 2

[0041] (1) Weigh 8g of polyvinyl alcohol (PVA) with a degree of polymerization of 1700-1800, add 0.25g of sodium alginate (SA) into 100mL of distilled water and mix, heat in a water bath to 90°C, and keep stirring with a glass rod until it is completely dissolved (no visible tiny particles), and keep warm for 5h to form a mixed gel solution of PVA and SA, and the final percentages of PVA and SA are 8% and 0.25%;

[0042] (2) After the PVA and SA mixed gel solution is cooled to room temperature, the activated sludge sludge water mixture is centrifuged to obtain wet bacteria, and the concentrated bacteria suspension is prepared in step (1) according to the ratio of 1:2 of the bacteria content. The mixed gel solution is mixed to obtain a mixed bacterial solution;

[0043] (3) Weigh 6g of boric acid (room temperature 25°C) and 2g of calcium chloride and dissolve them in 100mL of distilled water to prepare a crosslinking agent solution;

[0044] (4) Use a 10mL syringe to drop the ...

Embodiment 3

[0047] (1) Weigh 10g of polyvinyl alcohol (PVA) with a degree of polymerization of 1700-1800, add 0.25g of sodium alginate (SA) into 100mL of distilled water and mix, heat in a water bath to 90°C, and keep stirring with a glass rod until it is completely dissolved (no visible tiny particles), and keep warm for 5 hours to form a mixed gel solution of PVA and SA, and the final percentages of PVA and SA are 10% and 0.25%;

[0048] (2) After the PVA and SA mixed gel solution is cooled to room temperature, the activated sludge sludge water mixture is centrifuged to obtain wet bacteria, and the concentrated bacteria suspension is prepared in step (1) according to the ratio of 1:2 of the bacteria content. The mixed gel solution is mixed to obtain a mixed bacterial solution;

[0049] (3) Weigh 6g of boric acid (room temperature 25°C) and 2g of calcium chloride and dissolve them in 100mL of distilled water to prepare a crosslinking agent solution;

[0050] (4) Use a 10mL syringe to dr...

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Abstract

The invention belongs to the technical field of environment engineering and discloses polyvinyl alcohol immobilized microorganism gel beads and a preparation method and use thereof. The preparation method comprises adding polyvinyl alcohol and sodium alginate into water, carrying out stirring dissolution to obtain a uniform mixed gel liquid, mixing a microbe condensed suspension and the mixed gel liquid to obtain a mixed bacterial liquid, dropwisely adding the mixed bacterial liquid into a calcium chloride-containing saturated boric acid solution along with stirring, then carrying out a full crosslinking process at a temperature of 0-10 DEG C and washing the beads by distilled water so that the polyvinyl alcohol immobilized microorganism gel beads are obtained. The polyvinyl alcohol immobilized microorganism gel beads have high mechanical properties, good mass transfer performances, long biological activity lasting time and a good application prospect in the field of sewage treatment.

Description

technical field [0001] The invention belongs to the technical field of environmental engineering, and in particular relates to polyvinyl alcohol-immobilized microbial gel beads and a preparation method and application thereof. Background technique [0002] Immobilized microorganism technology is a new biotechnology developed on the basis of immobilized enzyme technology. It can greatly increase the concentration of microorganisms, make microorganisms not easy to lose and use for a long time, and their anti-toxicity and tolerance are significantly increased. Solid-liquid separation is easy, less secondary pollution. It can effectively treat organic wastewater, ammonia-containing wastewater, heavy metal wastewater and dye wastewater, and has excellent development prospects. [0003] The methods of microbial cell immobilization mainly include adsorption method, carrier binding method, cross-linking method and embedding method. Among them, the embedding method is the most comm...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N11/08C12N11/04C02F3/34
Inventor 牛晓君王杰
Owner SOUTH CHINA UNIV OF TECH
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