Fermentation method for improving yield of spinosad

A technology of spinosad and fermentation method, which is applied in the field of microbial fermentation, can solve the problems of not meeting the requirements of industrialization and low fermentation yield, and achieve the effects of weakening repression, good adsorption, and lower concentration

Inactive Publication Date: 2016-03-30
牡丹江佰佳信生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in my country, spinosyn has not yet achieved industrial production, the main r

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 improves the fermentation method of spinosad output

[0031] Saccharopolysporaspinosa (Saccharopolysporaspinosa) Z68 is used to ferment and produce spinosyn, and the fermentation medium used is: corn starch 30g / L, glucose 40g / L, corn steep liquor 18g / L, cottonseed cake powder 15g / L, yeast extract powder 3g / L, 20g / L soybean powder (through 400 mesh sieve), 5g / L calcium carbonate, 5g / L soybean oil and 10g / L macroporous resin, prepared with water.

[0032] Using the above medium, ferment in a 330-ton fermenter according to the following conditions: culture temperature 28°C, stirring speed 200rpm, aeration ratio 1:0.5, tank pressure 0.03MPa, dissolved oxygen controlled above 40%, and a fermentation period of 192hr. At 96 hours after the start of fermentation, add whole milk powder for the first time to a final concentration of 15g / L, and at 144 hours after the start of fermentation, add whole milk powder for the second time to a final concentration of 5g / L.

...

Embodiment 2

[0043] Embodiment 2 improves the fermentation method of spinosad output

[0044] Spinosyn was produced by fermentation of S. spinosa Z68. The fermentation medium used was: corn starch 20g / L, glucose 30g / L, corn steep liquor 15g / L, cottonseed cake powder 10g / L, yeast extract powder 1g / L , soybean powder (400 mesh sieve) 5g / L, calcium carbonate 4g / L, soybean oil 4g / L and macroporous resin 5g / L, prepared with water.

[0045] Using the above medium, ferment in a 330-ton fermenter under the following conditions: culture temperature 26°C, stirring speed 150rpm, aeration ratio 1:0.3, tank pressure 0.03MPa, dissolved oxygen controlled above 40%, and a fermentation period of 168hr. At 96 hours after the start of fermentation, add whole milk powder for the first time to a final concentration of 5g / L, and at 144 hours after the start of fermentation, add whole milk powder for the second time to a final concentration of 5g / L.

[0046] After 168 hours of fermentation, the spinosad fermentat...

Embodiment 3

[0047] Embodiment 3 improves the fermentation method of spinosad output

[0048] Spinosyn was produced by fermentation of S. spinosa Z68. The fermentation medium used was: corn starch 40g / L, glucose 50g / L, corn steep liquor 20g / L, cottonseed cake powder 20g / L, yeast extract powder 5g / L , soybean powder (400 mesh sieve) 30g / L, calcium carbonate 6g / L, soybean oil 6g / L and macroporous resin 15g / L, prepared with water.

[0049] Using the above medium, ferment in a 330-ton fermenter according to the following conditions: culture temperature 30°C, stirring speed 220rpm, aeration ratio 1:0.7, tank pressure 0.04MPa, dissolved oxygen controlled above 40%, and fermentation cycle 216hr. At 96 hours after the start of fermentation, add whole milk powder for the first time to a final concentration of 20g / L, and at 144 hours after the start of fermentation, add whole milk powder for the second time to a final concentration of 15g / L.

[0050] After 216 hours of fermentation, the spinosad fe...

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PUM

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Abstract

The invention provides a fermentation method for improving yield of spinosad. Macroporous resin is added into a fermentation medium of Saccharopolyspora spinosa, whole milk powder is added during a fermentation process, so that the yield of the spinosad is improved. The macroporous resin added in the fermentation medium can effectively and rapidly reduce concentration of the spinosad, and decrease repression of metabolite; the whole milk powder added as supplementary food in the fermentation process is good for continue growth and product synthesis of bacteria, and can further improve yield of the spinosad.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a fermentation method for improving the yield of spinosyns. Background technique [0002] Spinosyn, a secondary metabolite produced by the aerobic fermentation of Saccharopolyspora spinosa, belongs to macrolide compounds, and the effective active ingredient in its commercial products is spinosad A (85%-90%) and spinosyn D (10%-15%). Spinosad has no antibacterial activity, but it has good insecticidal activity. It has a wide insecticidal spectrum against pests of Lepidoptera and Thysanoptera. Lepidopteran pests have a good control effect. Spinosad is one of the most selective compounds among existing insecticides in the control of Lepidoptera pests, and its activity is equivalent to cypermethrin. While spinosyn has high insecticidal activity, it exhibits low toxicity to non-target organisms. It has low toxicity to mammals, birds and beneficial insects, only slightly toxic t...

Claims

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Application Information

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IPC IPC(8): C12P19/62C12R1/645
Inventor 王书睿周贤龙刘静
Owner 牡丹江佰佳信生物科技有限公司
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