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Acephate colloidal gold detection card

A technology of acephate and colloidal gold, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of improper storage and use, human and animal poisoning, etc., and achieve the effects of saving detection costs, accurate results and high sensitivity

Inactive Publication Date: 2016-04-13
JIANGSU WISE SCI & TECH DEV
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Problems solved by technology

Improper storage and use can cause human and animal poisoning

Method used

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  • Acephate colloidal gold detection card
  • Acephate colloidal gold detection card
  • Acephate colloidal gold detection card

Examples

Experimental program
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Embodiment Construction

[0012] 1. Preparation of Labeled Colloidal Gold

[0013] Preparation of colloidal gold solution: 1 g of chloroauric acid (HAuCl 4 ) powder dissolved in 100mL ultrapure water to prepare 1% chloroauric acid solution; then take 1L ultrapure water and heat it on a special electric furnace, add 10mL 1% trisodium citrate aqueous solution after boiling, continue heating for 5 minutes Then add 10mL of 1% chloroauric acid solution, heat it to boiling again, wait for the color to turn into wine red, then start timing, stop heating after 10 minutes, take it off, cool naturally, and set the volume to 1L, which is the colloidal gold solution.

[0014] Antibody labeling: 0.1mol / lK required for determination of antibody 2 CO 3 ratio, take 100mL colloidal gold solution, add this ratio of 0.1mol / L K 2 CO 3 , mix well (about 5min), then dissolve acephate and the second species of animal protein with PBS (0.01mol / L, pH7.4) to dissolve and dilute to 2mg / mL, add 2mL of 2mg / mL acephate Antibod...

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Abstract

The invention relates to an acephate colloidal gold detection card. The acephate colloidal gold detection card is mainly used for rapidly detecting acephate content in food. According to the method, a sample liquid absorbing part, a colloidal gold labeling part, a detection reaction part and a water absorbing part are orderly stuck on a backing of test paper. The detection reaction part is coated by an antigen 1 bar which is applied to detection and is used as a detection line, and at the same time is coated by an IgG1 bar which resists the second species of animal protein and is used as a reference line. The rapid detection test paper bar has high specificity, can carry out semi quantitative detection, can be used at the environmental temperature of 4 to 35 DEG C, and is suitable for an individual farmer, a food hygiene and quality inspection department, customs and other animal source food to carry out rapid detection on acephate residues. The acephate colloidal gold detection card provided by the invention has the advantages of high specificity, high sensitivity, simple and convenient operation and the like, and can carry out semi quantitative detection.

Description

technical field [0001] The invention belongs to the field of food safety detection, and specifically relates to a detection method of harmful residues in food, especially a detection method of acephate test paper. Background technique [0002] Acephate (English name: Acephate), also known as acephate, is a low-toxicity insecticide. Acephate is a systemic insecticide, which has stomach poisoning and contact killing effects, and can kill eggs. It has a certain fumigation effect and is a slow-acting insecticide. It is suitable for vegetables, tea trees, tobacco, fruit trees, cotton, rice , wheat, rapeseed and other crops to prevent and control a variety of chewing, piercing and sucking mouthpart pests, harmful mites and sanitary pests. Improper storage and use can cause human and animal poisoning. [0003] Acephate is an organophosphate pesticide. This type of pesticide inhibits cholinesterase in the body, causing neurophysiological dysfunction. Acute poisoning of organopho...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558
Inventor 洪霞薛永来吴明慧
Owner JIANGSU WISE SCI & TECH DEV
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