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Seed cryopreservation method

A storage method and cryogenic technology, applied in the fields of botanical equipment and methods, preservation and application of plants, can solve problems such as large investment and cost, and achieve the effects of small footprint, improved vitality, and increased resistance

Active Publication Date: 2016-04-20
DALIAN BOYUAN SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of different plant materials are different after being stored in the same cryogenic method. Different cryopreservation systems need to be established. At the same time, the investment in equipment in the early stage of library construction is also relatively large.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] After the corn seeds were frozen, processed and stored, the seed emergence rate and plant growth were affected. We selected a total of 12 main planting corn varieties suitable for each accumulated temperature zone in Heilongjiang Province (two main planting varieties for each accumulated temperature zone) for experiments (see Table 1). .

[0027] Table 1 Selected Planting Varieties

[0028]

[0029] experiment method:

[0030] 12 corn hybrid seeds were cryopreserved. The processing process is to freeze and soak the selected corn seeds in a freezing liquid at -15°C for 6 minutes, wipe off the freezing liquid after taking them out, and store them in a conventional refrigerator for 3 months. When planting, single seed was sown, and the seeds were subjected to two treatment methods, the first group was seeds treated at deep low temperature, and the second group was untreated seeds. The composition and weight percentage of the refrigerant liquid used are:

[0031] ...

Embodiment 2

[0045]In order to examine the growth of cucumber seeds after low-temperature freezing treatment, we chose the 09-11 Lianxiu cucumber cultivar cultivated by Dalian Agricultural Science Institute for the experiment. First, the cucumber seeds are subjected to low-temperature freezing treatment by using the deep-low temperature freezing treatment method described in the present invention, and then the low-temperature frozen cucumber seeds and the untreated cucumber seeds are planted in two areas of the same cultivated plot, and all planting conditions are consistent. As can be seen from the growth process of cucumber seeds, its growth potential of the seeds treated with low temperature freezing all exceeds that of the untreated seeds (see Figure 8-11 ).

Embodiment 3

[0047] Take the seeds of rice, soybean and pepper, and divide each seed into two groups: the low-temperature freezing treatment group and the untreated group. The seeds of the low-temperature freezing treatment group are wrapped with gauze, placed in freezing liquid, and soaked at -20°C Freeze for 8 minutes, remove the gauze after taking out the seeds, coat the seeds, pack them and put them in a conventional refrigerator for refrigerated storage for 3 months. The composition and weight percentage of the freezing liquid are: 15% sodium chloride, 26% ethanol with a concentration of 98%, 23% glycerin, 5% edible sucrose, 6% chitin, and the rest is water.

[0048] The seeds of the same kind of low-temperature freezing treatment group and the untreated group were planted in two areas of the same cultivated plot, and the planting conditions were consistent. It can be seen from the growth process of each plant that the growth vigor of the seeds treated by low temperature freezing all ...

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Abstract

The invention provides a seed cryopreservation method. The method is characterized in that seeds are wrapped in gauze, placed in refrigerating fluid to be soaked and frozen for 2-15 minutes at the temperature between minus 15 DEG C and minus 30 DEG C and taken out, the gauze is removed, and the seeds are covered, packaged and placed in a seed bank for refrigerated storage or placed in a ventilating and cool warehouse for storage. The method has the advantages that equipment cost is low, space is saved, speed is high, operation is easy and convenient, and the occupied area is small. The seeds processed through the method quickly meet the requirements for warehousing conditions such as drying dehydration; besides, the vitality of the seeds can be improved, the life of the seeds can be prolonged, the resistance of the seeds and plants can be enhanced, the effect of increasing both yield and revenue is achieved eventually, and the problem that in existing storage technologies, the drying process, temperature and humidity are hard to control together can be solved.

Description

technical field [0001] The patent belongs to the related field of plant seed storage, and the non-vernalized crop seeds are treated, and the treated seeds can quickly meet the requirements of storage conditions such as drying and dehydration, and maintain high seed vitality. The method has the advantages of low cost, space saving, high speed and simple operation process. Background technique [0002] Seeds are the main material for agricultural production, and the quality of seeds directly affects the efficiency and benefits of production. The lifespan of seeds varies. Most of the seeds of food crops and commercial crops are short-lived seeds. The seeds and germplasm resources are prone to aging and deterioration during storage. This brings difficulties to the preservation of seeds and germplasm resources, and not only affects the breeding units. The work will also increase the risk and cost input of the seed management unit. The country pays more and more attention to the...

Claims

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Application Information

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IPC IPC(8): A01N3/00
CPCA01N3/00
Inventor 李富岭张朝澍许振南陈曦张临新田思进国俊伍王光杰
Owner DALIAN BOYUAN SCI & TECH CO LTD
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