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Method for inducing differentiation of fibroblasts into multifunctional stem cells

A technology of pluripotent stem cells and fibroblasts, which is applied in the field of inducing fibroblasts to differentiate into pluripotent stem cells, and inducing fibroblasts in skin lesions of vitiligo patients into pluripotent stem cells

Active Publication Date: 2016-04-20
AFFILIATED HOSPITAL OF JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the somatic cells of many patients have been induced into pluripotent cells, but the use of somatic cells of vitiligo patients to induce pluripotent stem cells has not been reported in the literature

Method used

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  • Method for inducing differentiation of fibroblasts into multifunctional stem cells
  • Method for inducing differentiation of fibroblasts into multifunctional stem cells
  • Method for inducing differentiation of fibroblasts into multifunctional stem cells

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Embodiment Construction

[0031] The present invention will be further described below in conjunction with specific implementation examples.

[0032] Reagent materials to be used, the name of the source company in brackets;

[0033] DMEM / F12 (Gibco), KOSR (Gibco), Non-essentialaminoacids (Gibco), L-glutaMAX (Gibco), β-mercaptoethanol (Gibco), bFGF (Peprotech), DMEM (Hyclone), FBS (Gibco), Vc ( Sigma), polybrene (Sigma), GCDR (Stemcell), PBS (Hyclone), Y27632 (Selleck), AP reagent (Sigma), goat anti-human OCT4 (SantaCruz), rabbit anti-human NANOG (Genetex), mouse anti-human TRA- 1-60 (Millipore), SSEA4 (dshb), donkey anti-sheep CY3, donkey anti-rabbit CY3, donkey anti-mouse CY5 (Jackson), CpGenomeTurboBisulfiteModification Kit (Millipore), reverse transcription and RT-PCR kit (Takara).

[0034] ES medium includes 80%DMEM / F12, 20%KSR, 1%Non-essentialaminoacids, 1mML-glutaMAX, 0.1mMβ-mercaptoethanol, 5ng / mLbFGF.

[0035] The method for inducing fibroblasts in skin lesions of vitiligo patients into pluri...

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Abstract

The invention relates to a method for inducing differentiation of fibroblasts into multifunctional stem cells, in particular to a method for inducing fibroblasts in a skin lesion position of a patient with vitiligo into multifunctional stem cells and belongs to the field of biotechnology. According to the method, the fibroblasts in the skin lesion position of the patient with vitiligo are induced into the multifunctional stem cells with a four-factor introduction method, the induced cells are identified, and the condition that the cells have characteristics of the stem cells and are similar to ES (embryonic stem) cells is determined; an ES cell source is in ethical dispute, the multifunctional stem cells induced from somatic cells of the patient are widely sourced, are free of immunological rejection, have corresponding disease attributes and can be used for disease study, drug screening and the like; the multifunctional stem cells can be induced from various somatic cells of the patient, and the multifunctional stem cells are induced from the fibroblasts in the skin lesion position of the patient with vitiligo for the first time.

Description

technical field [0001] The invention relates to a method for inducing fibroblasts to differentiate into multifunctional stem cells, in particular to a method for inducing skin fibroblasts in skin lesions of vitiligo patients to become multifunctional stem cells, and belongs to the field of biotechnology. Background technique [0002] Vitiligo (vitiligo) is a common local or systemic depigmentation skin disease. The incidence of the disease is about 0.5-2%. The pathogenesis of vitiligo is not very clear. In recent years, studies have related to genetic factors, autoimmune factors, oxidative stress response, self-destruction of melanocytes, and neuropsychiatric factors. There are many treatment methods for this disease, including drug therapy, physical therapy, surgical operation, etc., but the effect is not very satisfactory, which has a certain impact on the life of the patient. [0003] There have been many achievements in the research of disease treatment with stem cells ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/074C12N5/071
CPCC12N5/0656C12N5/0696C12N2506/09C12N2506/1307
Inventor 李遇梅李淑刘莉萍许辉惠利健郑允文
Owner AFFILIATED HOSPITAL OF JIANGSU UNIV