A cold tolerance gene qct-3-2 stably expressed at the booting stage of rice hhz and its molecular labeling method

Abstract

A cold tolerance gene qCT‑3‑2 stably expressed at the booting stage of rice HHZ The invention relates to a molecular marker method thereof, belonging to the fields of rice high-yield stress-resistant breeding and molecular genetics. According to the phenomenon of linkage and linkage disequilibrium, using the interrelated breeding population derived from "Huanghuazhan" and 8 donor parents with rich diversity, genome-wide association analysis and physical mapping methods were used to fine-map and verify the stable expression at booting stage Cold tolerance gene qCT‑3‑2 HHZ , to further develop a practical and economical marker ZCT‑7 based on common PCR. Applying the present invention to auxiliary selection breeding and aggregation breeding for rice tolerance to chilling damage can effectively make up for the deficiencies of natural variation genes in the past, not only can significantly reduce the negative impact of using the initial positioning results to carry out auxiliary breeding, but also can be used at the seedling stage. Genotype selection is performed on the low-generation breeding population to obtain cold-tolerant individuals, which facilitates timely hybridization and transfer, saves the process of identifying low-temperature resistance at the adult stage, improves breeding efficiency, and speeds up the breeding process.

Description

technical field [0001] The invention relates to a rice booting stage stably expressing cold tolerance gene qCT-3-2 HHZ And its molecular marker, the gene significantly increases the cold tolerance of rice at the booting stage and reduces the yield loss, belongs to the field of rice high-yield stress-resistant breeding and molecular genetics, and is suitable for introducing genes stably expressed at the booting stage in rice high-yield stress-resistant breeding qCT-3-2 HHZ , and use molecular markers for assisted selection breeding of the gene. Background technique [0002] Chilling injury is one of the most important abiotic stress factors that cause rice yield loss and limit the distribution of rice planting. At present, chilling damage occurs frequently, and the loss caused by chilling damage in China is as high as 3-5 million tons every year. Molecular genetic studies have shown that low temperature resistance in rice is a quantitative trait controlled by multiple gene...

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Problems solved by technology

However, there is no report on gene identification using this strategy in rice.

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