Method for performing banded sclerotial blight inoculation and resistance evaluation by means of rice detached leaves

Abstract

The invention discloses a method for performing banded sclerotial blight inoculation and resistance evaluation by precisely controlling banded sclerotial blight inoculation conditions of rice detached leaves. The method comprises the steps of rice planting, rhizoctonia solani, rhizoctonia solani inoculation, illness surveying and statistics after inoculation of rice detached leaves, sampling of rice materials inoculated with the rhizoctonia solani, rice RNA extraction, RT-PCR identification and the like. An experimental procedure for quantitatively evaluating the detached leaf morbidity degree and precisely conducting molecular detection is built, and therefore the screening and research efficiency of the rice banded sclerotial blight resistance material is improved.

Description

technical field [0001] The invention relates to rice sheath blight, in particular to a method for sheath blight inoculation and resistance evaluation using detached rice leaves. Background technique [0002] Rice sheath blight is one of the important diseases of rice. The pathogen is Rhizoctonia solani, which is a semi-saprophytic fungus with a wide range of hosts. It generally infects rice at the tillering stage under high temperature and high humidity conditions, and begins to infect and expand from the leaf sheath near the water surface. It can also harm leaves, stalks and even rice ears, causing a decrease in seed setting rate, and seriously causing plant death. Rice sheath blight is distributed in most rice growing areas and can cause yield losses of up to 50%. With the promotion of dwarf varieties, the increase of nitrogen fertilizer and climate change, the damage of rice sheath blight has gradually increased, and it has become a serious obstacle to the stable and hi...

AI Technical Summary

Problems solved by technology

The inoculation method of rice sheath blight at the adult plant stage in the field has good reliability and repeatability, but the environment controllability of field identification is poor, and the test period is longer due to seasonal restrictions

Rice sheath blight inoculation at the seedling stage has the advantages of small experimental scale, controllable pathogenic environment, and short identification period. However, it is difficult to grasp the time point for disease investigation of inoculated rice seedlings, and the difference in growth potential of different varieties will affect the identification results.

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