Supercharge Your Innovation With Domain-Expert AI Agents!

MiRNA marker for diagnosing cervical cancer and application thereof

A marker, cervical cancer technology, applied in the fields of genetic engineering and reproductive medicine, can solve problems such as no related reports, and achieve the effects of improving sensitivity and specificity, and facilitating early diagnosis and detection.

Inactive Publication Date: 2016-04-20
WUXI MATERNAL & CHILD HEALTH HOSPITAL
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the value of miR-320 as a relatively stable biomarker for the auxiliary early diagnosis of cervical cancer has not yet been reported. If miRNAs can be screened as biomarkers and corresponding auxiliary early diagnosis kits are developed, it will be beneficial to the development of cervical cancer in my country. The state of diagnostics will surely be a powerful push

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • MiRNA marker for diagnosing cervical cancer and application thereof
  • MiRNA marker for diagnosing cervical cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The collection of embodiment 1 sample and the arrangement of sample data

[0043] The inventor has collected cervical cancer patients and normal control cervical tissues from Wuxi Maternal and Child Health Hospital since 2010 (the samples used for research were collected at the same period, and the sampling, packaging and storage conditions were uniform), and all patients were confirmed by pathological examination And not combined with other inflammatory diseases and immune-related diseases, the normal controls were all hysterectomy due to uterine fibroids at the same time, the postoperative cervical histopathological examination report showed normal cervical epithelium, and no other diseases. The demographic and clinical data of these samples were collected systematically. A total of 75 eligible samples were used in this study.

Embodiment 2

[0044] Example 2 Detection of miR-320 in tissues

[0045] The above-mentioned 45 cases of cervical cancer and 30 cases of healthy controls were tested by PCR to obtain relevant results. The specific steps are:

[0046] 1. Take the tissues of patients in the "cervical cancer case" group and the "healthy control" group, add 3 times the volume of Trizol reagent, and crush the tissues with an ultrasonic homogenizer;

[0047] 2. Phase separation: place at room temperature for 15min, then add an equal volume of chloroform, shake for 50s, room temperature for 15min, 14,000rpm, 4°C, centrifuge for 15min;

[0048] 3. RNA precipitation: transfer the water phase to a new 15ml centrifuge tube, add 1.5 times the volume of the water phase in absolute ethanol, and mix well;

[0049] 4. Enrich RNA with QIAGENmiRNeasykit: pipette 700ul samples into the spin column each time, centrifuge at 14,000rpm for 15s, discard the filtrate in the collection tube, and repeat until all samples pass throug...

Embodiment 3

[0059] Example 3 Preparation of miR-320 kit for auxiliary early diagnosis of cervical cancer

[0060] The production and operation process of the miR-320 kit is based on technologies such as RT-PCR and real-timePCR. The kit includes miR-320 primers (primers for miR-320 are SEQIDNo.1 and SEQIDNo.2), and common enzymes and / or reagents required for corresponding PCR reactions, such as: reverse transcriptase, buffer, dNTPs, MgCl 2 , nuclease-free water, fluorescent probe, Taq enzyme, miR-320 reverse transcription primer (5'-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTCGCCCTC-3'), etc., can be selected according to the specific experimental method used. These commonly used enzymes and / or reagents are skilled in the art As is well known to personnel, there may also be standard products and controls (such as quantitatively standardized nematode miR-39 samples, etc.) and normal reference values.

[0061] The value of this kit is that it only needs a small amount of tissue samples, detects ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a miRNA marker for diagnosing cervical cancer and belongs to the technical field of genetic engineering and reproductive medicine. The miRNA marker is miR-320, and primers of the miRNA are SEQ ID No.1 and SEQ ID No.2. The marker and the primers thereof can be used for preparing a cervical cancer auxiliary early-diagnosis kit and used for cervical cancer early-stage auxiliary diagnosis and prognosis evaluation.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and reproductive medicine, and relates to a miRNA marker for diagnosing cervical cancer and its application, in particular to a miRNA marker related to cervical cancer auxiliary early diagnosis and prognosis evaluation and its application. Background technique [0002] Cervical cancer is the most common malignant tumor of the reproductive system in women, which seriously endangers women's health and life. In 2011, the latest statistics on cervical cancer published by Lancet showed that from 1980 to 2010, the global incidence of cervical cancer increased at an average annual rate of 0.6%. The total number of women reached more than 200,000, and the age of death tended to be younger. The grim reality revealed by the research results of cervical cancer epidemiology makes it urgent to find more sensitive and specific molecular markers for clinical diagnosis and prognosis evaluation of cer...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 张婷陈道桢邹萍陈钰蒋洁
Owner WUXI MATERNAL & CHILD HEALTH HOSPITAL
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com