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Detection method of bacterium content in high-concentration microbial preparation product

A technology of microbial preparation and detection method, applied in the field of microbiology inspection, can solve the problem of inaccurate bacterial content, and achieve the effects of accurate measurement, reduced error and easy to master

Inactive Publication Date: 2016-04-27
北京世纪阿姆斯生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for detecting the bacterial content of high-concentration microbial preparation products, which can solve the problem of inaccurate detection of the bacterial content of high-concentration microbial preparations in the prior art

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  • Detection method of bacterium content in high-concentration microbial preparation product

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Embodiment 1

[0046] A method for detecting the bacteria content of a high-concentration microbial preparation product, comprising:

[0047] S11. Weigh the sample of the microbial preparation and add it into the solvent for mixing to obtain the first dilution;

[0048] S12. Draw the first dilution solution into the solvent and shake to obtain the second dilution solution;

[0049] S13, repeating step S12 eight times, drawing and diluting each time from the dilution obtained last time, to obtain the 3rd, 4th, 5...10 dilutions respectively;

[0050] S14. Take 4 serial dilutions of the bacterial solution and evenly spread it on the surface of the culture medium to cultivate and count the number of colonies. Combined with the dilution factor and the volume of the diluted solution drawn during the plate coating, the bacterial content of the high-concentration microbial preparation product is obtained.

Embodiment 2

[0052] A method for detecting the bacterial content of high-concentration microbial preparation products, taking the detection of 150 billion / mL bacillus preparations as an example, including:

[0053] S21. Weigh 1.000 mL of the bacillus preparation sample and add it to 9 mL of solvent, and incubate on a shaker at 300 rpm for 40 minutes to obtain the first dilution;

[0054] S22. Draw 1 mL of the first dilution and add it to 9 mL of solvent, and use a small vortex shaker to oscillate at 80 Hz for 40 seconds to obtain the second dilution;

[0055] S23, repeating step S22 eight times, drawing and diluting each time from the dilution obtained last time, to obtain the 3rd to 10th dilutions respectively;

[0056] S24. Take the 7th, 8th, 9th, and 10th dilutions of the bacterial solution and the same amount of the solvent and evenly spread them on the surface of the culture medium to cultivate and count the number of colonies, and combine the dilution factor and the volume of the dilut...

Embodiment 3

[0058] A method for detecting the bacterial content of high-concentration microbial preparation products, taking the detection of 118 billion / g Bacillus subtilis preparations made by spray drying as an example, including:

[0059] S31. Weigh 1.000g of the bacillus preparation sample with an electronic balance, add it into a 18×180mm sterile test tube with about 10 glass beads, add 9ml of sterile water, and then shake it with a shaker at 200 rpm for 30 minutes. Form a 1:10 dilution;

[0060] S32. In the ultra-clean workbench of the aseptic room, use a pipette gun to draw 1ml of the above diluent and slowly add it into a 18×180mm sterile test tube containing 9ml of sterile water (note that the tip of the pipette should not touch the sterile water in the tube), and use Oscillate with a small vortex oscillator at 60Hz for 30 seconds, mix to 1:1×10 2 The diluted bacterial suspension;

[0061] S33. Repeat the previous step, and dilute sequentially to obtain... 1:1×10 7 , 1:1×10 ...

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Abstract

The invention relates to the technical field of microbiological test, in particular to a detection method of bacterium content in a high-concentration microbial preparation product. The detection method comprises the following steps: 1) weighing a microbial preparation sample and adding the weighed microbial preparation sample to a solvent, and oscillating so as to obtain first thinned liquid; 2) sucking the first thinned liquid into the solvent and oscillating so as to obtain second thinned liquid; 3) repeating the step 2) for n times, and sucking thinned liquid obtained from previous time and thinning in each time, so as to obtain thinned liquids of the 3rd, 4th, 5th and until (n+2)th times; and 4) uniformly coating bacterium liquids of 2-4 continuous thinning degrees on the surface of a culture medium, culturing and calculating the number of colonies; inducing backwards in accordance with a thinning time and the volume of the thinned liquid which is sucked when coating the plate, so that the bacterium content of the high-concentration microbial preparation product is obtained. By the method, errors caused by gradient thinning can be reduced and measurement can be more accurate; and the method is suitable for measuring 1-150 billions / g or 1-150 billions / ml of microbial preparations.

Description

technical field [0001] The invention relates to the technical field of microbiological testing, in particular to a method for detecting bacteria content of high-concentration microbial preparation products. Background technique [0002] The application of microbial preparations in agriculture is mainly in the process of planting and breeding. The main functions in planting are to promote plant growth, improve plant disease prevention and resistance, improve fertilizer utilization rate, improve plant drought resistance, repair soil, degrade pesticides, etc. ; The main role in breeding is to supplement, adjust or maintain the micro-ecological balance in the intestinal tract of animals, promote the intestinal absorption of the body and improve the immune level of the host; it can also adjust and repair the water environmental pollution caused by intensive farming , to maintain the ecological balance of the breeding environment. [0003] Due to the limitation of the preparation...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 李艳萍邓祖科李伏明刘庆商宇红魏丽华温国昌李阔
Owner 北京世纪阿姆斯生物技术有限公司
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