Cannabis sailve extract rich in cannabidiol and preparation method of cannabis sailve extract

[0034] 1) Preparation of sample solution

[0035] Accurately weigh 0.1 g of the extract product, add 5 ml of chloroform to dissolve it into a solution, then pass the 0.22 μm organic membrane after ultrasonic bathing for 5 minutes, and place the filtrate in the refrigerator for later use.

[0036] 2) Determination of CBD content

[0037] The Agilent HP-5 column and Agilent 7890A gas analyzer were used for the measurement. The chromatographic conditions are as follows: the carrier gas is helium, the flow rate is 1.00 ml/min; the column temperature program: the starting temperature is 100°C, keep for 5 minutes, program the temperature to 250°C at 10°C/min, and the temperature to 300°C at 5°C/min. Hold for 10 minutes; the inlet temperature is 150°C, the detector temperature is 300°C, and the split ratio is 5:1; the hydrogen flow rate is 40 ml/min, and the air flow rate is 400 ml/min; automatic sample injection, with a sample volume of 4.0 μL. Prepare standard solutions of different concentrations, perform the above operations, and draw a peak area-CBD concentration standard curve. According to the above method, the injection volume of the sample solution is 4.0 μL, and the CBD content in the extract product is calculated according to the standard curve.

[0038] Example 1

[0039] Spread the dried hemp flower leaves in an oven for dry heat treatment at a temperature of 80°C for 2 hours, and then grind them with a pulverizer. Take 1.5 kg of dry heat-treated hemp flower leaves, pack them with several filter bags, and place them in the extraction tank of the subcritical extraction device. According to the material-to-liquid ratio 1:8 (W/V), the industrial-grade n-butane in the solvent tank Pour into the material tank, extract for 40 minutes in a subcritical state with a temperature of 35°C and an extraction pressure of 0.4 MPa, and repeat the extraction three times to obtain a crude extract. The crude extract was dissolved in ethanol according to a solid-liquid ratio of 1:5 (W/V); then it was placed in a refrigerator at -10°C for 60 minutes of winterization. Then transfer to a centrifuge with a temperature of -6°C in advance and a rotation speed of 4500 rpm, and perform centrifugation for 30 minutes. Activated carbon was added to the supernatant for decolorization, the amount was 6% of the mass of the crude extract, and then placed on a shaker with a rotation speed of 120 rpm for 30 minutes. Then, the above mixture is filtered to obtain a filtrate. The filtrate was placed in a rotary evaporating flask, and the rotary evaporating process was performed under the conditions of a water bath temperature of 45°C and a rotation speed of 50 rpm. After the ethanol rotary steaming, the hemp extract rich in CBD can be obtained. The yield of hemp extract was 3.0%. Gas chromatographic detection revealed that the extract contained CBD at 18.21% (W/W).

[0040] Example 2

[0041] Spread the dried hemp flower leaves in a general oven for dry heat treatment at a temperature of 80°C and a time of 2 hours. Then grind it with a pulverizer. Take 1.5 kg of dried hemp flower leaves, divide them with filter bags, and place them in the extraction tank of the subcritical extraction device. First measure 95% ethanol into the extraction tank, and send the corresponding volume of n-butane through the flow valve of the equipment , Make the concentration of ethanol in the solvent tank reach 20% of n-butane, pour the mixed n-butane solvent into the material tank, and extract for 40 minutes at a temperature of 40°C and an extraction pressure of 0.4MPa in a subcritical state, and the extraction temperature is 55°C , Extract for 40 minutes, then desolvate under reduced pressure, take out the extract, and repeat the extraction 3 times. Add a certain amount of ethanol to the obtained ethanol solution containing the extract to achieve a solid-liquid ratio of 1:7, and then place it in a refrigerator at -10°C for 45 minutes of winterization. Centrifuge for 30 minutes in a centrifuge at -10°C and a rotation speed of 4000 rpm. Activated carbon was added to the obtained supernatant for decolorization, the amount of which was 8% of the mass of the crude extract, and then placed on a shaker with a rotating speed of 120 revolutions per minute for 20 minutes. Then, the above mixture is filtered to obtain a filtrate. The filtrate is placed in a rotary evaporating flask, and the rotary evaporation is performed under the conditions of a water bath temperature of 50° C. and a rotation speed of 45 revolutions/minute. After the ethanol rotary steaming, the hemp extract rich in CBD can be obtained. The yield of hemp extract was 5.2%. Gas chromatography found that the extract contained 25.22% CBD.