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Flavone-based fluorescent molecular probe and preparation method and application thereof

A fluorescent molecular probe, flavonoid-based technology, applied in biochemical equipment and methods, fluorescence/phosphorescence, chemical instruments and methods, etc., can solve the problems of complex electrical instruments, sample background interference, tedious sample preparation process, etc. The effect of sensitivity

Inactive Publication Date: 2016-05-04
SHENZHEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a flavonoid-based fluorescent molecular probe and its preparation method and application, aiming at solving the need for complex electrical instruments and tedious sample preparation process in the existing copper ion detection method, And it is very susceptible to the problem of sample background interference

Method used

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  • Flavone-based fluorescent molecular probe and preparation method and application thereof
  • Flavone-based fluorescent molecular probe and preparation method and application thereof
  • Flavone-based fluorescent molecular probe and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0052] 1) Synthesis of N-ethylflavone dye (DPA-FL)

[0053] Add 10mmol of synthesized N-ethyl intermediate C and 100mmol of bromomethylpyridine to the reaction flask at a molar ratio of 1:10, then add 100mmol of potassium carbonate and 10mmol of potassium iodide in turn, and finally add 20 ml of dry DMF, and react in Stir at 80 degrees Celsius for 48 hours. After the reaction is completed, the reaction solution is separated, extracted, washed, and dried to obtain a crude product, which is separated by column chromatography (eluent: methanol: dichloromethane = 1:9) to obtain N-ethyl Flavonoid dye (DPA-FL), the yield is 24%;

[0054]

[0055] 2) Spectrum test of DPA-FL

[0056] a. Preparation of dye mother liquor:

[0057] Add 1mmol of DPA-FL to 1mL of chromatographically pure DMSO, and fully dissolve it under ultrasound to prepare a 1mM DPA-FL mother solution. Transfer the mother solution to a brown glass bottle, seal and refrigerate.

[0058] Take 20 microliters of mothe...

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Abstract

The invention discloses a flavone-based fluorescent molecular probe and a preparation method and application thereof. The flavone-based fluorescent molecular probe has a structure shown as the following general formula I: (see the specifications) I, wherein R1 is H or CH3; and R2 is CH3, CH2CH3 or (CH2)3CH3. Compared with the traditional method, the flavone-based fluorescent molecular probe of a flavone dye is a convenient and rapid detection way with high sensitivity and high selectivity, not only can analyze the heavy metal polluted water source, but also can be applied to a biological system, and can conveniently, rapidly, high-sensitively and high-selectively detect free copper ions in living cells.

Description

technical field [0001] The invention relates to the technical field of fluorescent molecular probes, in particular to a flavonoid-based fluorescent molecular probe and its preparation method and application. Background technique [0002] Copper ions are one of the indispensable trace elements in the human body, and are extremely important catalytic cofactors in the metabolism of organisms. At the same time, they play a key role in biochemical processes such as gene expression, protein folding, and nervous system functionalization. However, there are also strict standards for the content of copper ions in the human body, too much or too little will cause harm to various functions of the human body. Copper ion imbalance in the human body is closely related to many diseases, such as Mank's disease, Asenheimer's disease, Wilson's disease, gastrointestinal disorders, and kidney disease. With the rapid advancement of human industrialization, copper has been widely used as an extr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C07D405/14G01N21/64C12Q1/02
CPCC07D405/14C09K11/06C09K2211/1007C09K2211/1029C09K2211/1088C12Q1/02G01N21/64
Inventor 赵晨刘斌
Owner SHENZHEN UNIV