How to store and transport immune cells

Abstract

The invention discloses a method for preserving and transporting immune cells and belongs to the technical field of biology. The method comprises the following steps of collecting peripheral blood or umbilical cord blood, and conducting dilution, so that cell suspension is obtained; adding a separating medium containing trehalose, ultra-low-viscosity sodium alginate and amino acid into a centrifuge tube, laying the cell suspension flat on the separating medium, conducting centrifugation, absorbing upper layer blood plasma for use, and absorbing albuginea layer cells, so that lymphocyte is obtained; mixing the lymphocyte with a cryopreservation solution in the same proportion, and placing the mixture into a cell cryopreservation tube; conducting cryopreservation; after the lymphocyte thaws, moving the liquid cell suspension into a centrifuge tube containing normal saline with the temperature ranging from 4 DEG C to 8 DEG C, mixing the mixture uniformly, and conducting centrifugation; adding preserving fluid containing trehalose with the concentration being 2% to the lymphocyte; moving the mixture into a sterile tube or a transfer bag for transfer. According to the method, raw materials meeting the intravenous injection level bulk pharmaceutical chemical standards are selected for all stages from separation to cryopreservation and then to preservation and transportation, uncertainty of the raw materials is low, and safety is high.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for storing and transporting immune cells. Background technique [0002] Immune cells not only have good prospects in the treatment of cancer, but also from the perspective of health preservation, immune cells can also be extracted from the peripheral blood of the patient when he is healthy, and the patient himself has immunocompromised or other symptoms in the future When the time comes, the stored immune cells can be reinfused back to the patient to improve the patient's immunity. The research and clinical application of immune cells is a major and complex task coordinated by multiple disciplines, units, and departments. It is necessary to have an independent cell preparation unit to provide cells, an independent cell transplantation unit to perform cell transplantation, and a cell preparation unit There is a certain space distance between the unit and the cell transplant...

AI Technical Summary

Problems solved by technology

The research and clinical application of immune cells is a major and complex task coordinated by multiple disciplines, units, and departments. It is necessary to have an independent cell preparation unit to provide cells, an independent cell transplantation unit to perform cell transplantation, and a cell preparation unit There is a certain space distance between the unit and the cell transplantation unit. In order to make the cell preparation unit and the cell transplantation unit fully connected in the work, so that the cell transplantation can achieve the best performance, there must be a standardized cell storage and transportation process that can meet the standards. The original immune cells that need to be stored and transported have very strict requirements in terms of storage, transport, and cells. At present, there is no unified standardized process for the storage and transportation of immune cells.

Therefore, during the process of separation, cryopreservation, resuscitation, and transportation, immune cells will cause damage or even toxicity to individual cells. risk; and the isolation rate and purity of isolated cells also have a great impact on the application of immune cells

[0003] In the prior art, conventional Ficoll 400 separation fluid has certain toxicity to cells, which is not conducive to clinical application, and the survival rate of cells after cryopreservation and recovery is usually between 70% and 90%.