Test-tube plantlet proliferation culture medium of pear tree somatic embryos

Abstract

The invention provides a test-tube plantlet proliferation culture medium of pear tree somatic embryos. The culture medium comprises 1 / 2 improved SH, 0.5-1.0mgL<-1> of 6-BA, 1-2mgL<-1> of 2,4-D, 0.3-0.5mgL<-1> of NAA, 6.0-7.0gL<-1> of agar, 30gL<-1> of sucrose and 0.1-0.3% of phytagel and has pH value of 5.8-6.0. The culture medium has the beneficial effects that pear trees difficult to propagate are rapidly propagated through somatic embryogenesis by improving a basic culture medium and components and adjusting the culture conditions; the plant transplanting survival rate is more than or equal to 96%; seedlings grow robustly, thus the problem of germplasm degeneration of good seedlings can be effectively solved, plenty of high-quality purified and rejuvenated seedlings can be also provided for production and an ideal receptor system can be also provided for breeding new varieties through genetic transformation or mutation breeding of the pear trees.

Description

technical field [0001] The invention relates to a value-added culture medium for in vitro seedlings of pear tree leaves in vitro, belonging to the field of botany. Background technique [0002] Pear trees belong to the genus Pyrus in the family Rosaceae, perennial deciduous fruit trees and trees, and are widely distributed around the world. There are about 35 native species in the world. They are wildly distributed in Europe, Asia and Africa. They are classified into two types: Oriental pear and Western pear. kind. China's pear cultivation area and output are second only to apples. [0003] Pear tree is a kind of tree species with high development value. At present, the propagation method usually adopted is cutting propagation, and this propagation method is susceptible to fungal diseases such as leaf spot. It is also vulnerable to aphids in spring and summer. The virus Seriously, leaf mottling is easy to fall off, and the degree of improved varieties is reduced; and the u...

AI Technical Summary

Problems solved by technology

[0003] Pear tree is a kind of tree species with high development value. At present, the propagation method usually adopted is cutting propagation, and this propagation method is susceptible to fungal diseases such as leaf spot. It is also vulnerable to aphids in spring and summer. The virus Seriously, the leaf mottling is easy to fall off, and the degree of improved variety is reduced; and the use of inferior seedlings leads to the loss of the inherent good characteristics of pear trees, which restricts the development and popularization of this tree species to a certain extent. Therefore, selection, breeding Excellent pear tree strains have become a very prominent production problem. Tissue culture rapid propagation is an effective way for the industrialization of improved pear tree varieties, and there are many related reports. Systematic technical research has no successful reports