Method for degrading methyl orange by means of photo-catalytic oxidation by aid of CYP119 enzymes loaded with TiO2-phenanthroline
A CYP119, photocatalytic oxidation technology, applied in chemical instruments and methods, biochemical equipment and methods, enzymes, etc., to achieve the effect of maintaining enzyme activity, low cost, and easy availability of raw materials
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Embodiment 1
[0018] Mix 3mL of n-butyl titanate with 10mL of ethanol to obtain a uniform solution A; dissolve 0.06g of o-phenanthroline in 10mL of ethanol to obtain a uniform solution B; slowly add solution B to A under constant stirring solution, a homogeneous solution was obtained. The obtained solution was added into a hydrothermal reaction kettle, and reacted at 180° C. for 24 hours. Centrifuge the obtained solid, wash it with deionized water and ethanol alternately for more than three times, dry it, and grind it. Mix 50mg of the powder obtained by grinding with 20mL of 1uM CYP119 enzyme solution, stir at 10°C for 12h, centrifuge, and wash to obtain the product.
Embodiment 2
[0020] Mix 6mL n-butyl titanate solution with 20mL ethanol to obtain a uniform solution A; dissolve 0.12g o-phenanthroline in 20mL ethanol to obtain a uniform solution B; slowly add solution B to A under constant stirring solution, a homogeneous solution was obtained. The obtained solution was added into a hydrothermal reaction kettle, and reacted at 180° C. for 24 hours. Centrifuge the obtained solid, wash it with deionized water and ethanol alternately for more than three times, dry it, and grind it. Mix 100mg of powder obtained by grinding with 40mL of 1uM CYP119 enzyme solution, stir at 10°C for 12h, centrifuge, and wash to obtain the product.
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