A kind of enzymatic hydrolysis method containing cellulose raw material

A technology of cellulase and cellulose, applied in biochemical equipment and methods, enzymology/microbiology devices, specific-purpose bioreactors/fermentation tanks, etc., can solve the problem of poor enzymatic hydrolysis effect and low sugar production efficiency , low enzyme utilization efficiency and other issues, to achieve the effect of uniform regulation, enhanced enzyme mixing efficiency, and improved enzyme utilization

Active Publication Date: 2019-05-17
COFCO NUTRITION & HEALTH RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide an enzyme containing cellulose raw material in order to overcome the defects of low enzyme utilization efficiency, poor enzymolysis effect and low sugar production efficiency in the enzymatic hydrolysis method of cellulose-containing raw material in the prior art. This method can effectively improve the utilization rate of enzymes, improve the efficiency of enzymatic hydrolysis and the yield of monosaccharides

Method used

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  • A kind of enzymatic hydrolysis method containing cellulose raw material
  • A kind of enzymatic hydrolysis method containing cellulose raw material
  • A kind of enzymatic hydrolysis method containing cellulose raw material

Examples

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Comparison scheme
Effect test

Embodiment 1

[0104] This example is used to illustrate the enzymatic hydrolysis method of the cellulose-containing raw material of the present invention.

[0105] (1) Pretreatment of cellulose-containing raw materials

[0106] Cut 2,222 kg of impurity-free corn stalks (moisture content 10% by weight) into small sections not exceeding 1.2 cm x 0.5 cm x 1.0 cm, maintain a pressure of 1.0 MPa at 180°C for 5 minutes, and then release the pressure to complete steam explosion . A total of 4000 kg of steam explosion waste (water content 50% by weight) was obtained.

[0107] Determination of the total weight of cellulose and the total weight of hemicellulose in the resulting solid steam explosion product:

[0108] Take 10 grams of the above-mentioned steam explosion product and dry it at 45° C. to a constant weight of 5 grams, weigh 300.0 mg of the dried steam explosion product, and place it in a 100-milliliter dry Erlenmeyer flask weighing 80 grams. Add 3.00 ml of sulfuric acid solution with a...

Embodiment 2

[0118] This example is used to illustrate the enzymatic hydrolysis method of the cellulose-containing raw material of the present invention.

[0119] Carry out the enzymolysis of cellulose-containing raw materials according to the method of Example 1, the difference is that in the main enzymolysis step, all the pre-mixed materials are added to an enzymolysis tank containing a viscosity of 200 cp enzymolysis liquid at one time, Then mix evenly with cellulase and hemicellulase (the weight ratio of the whole steam explosion product to the enzymolysis solution is 1.4:1, the pH value is 5.5, and the enzymolysis temperature is 50 ° C, relative to the dry weight of each gram of cellulose raw material Weight, the consumption of cellulase is 20 enzyme activity units, and the consumption of hemicellulase is 10% of cellulase weight), the consumption of cellulase and hemicellulase is identical with embodiment 1, and enzymolysis time is 72 Hour. Among them, the sugar concentration content...

Embodiment 3

[0124] This example is used to illustrate the enzymatic hydrolysis method of the cellulose-containing raw material of the present invention.

[0125] Carry out the enzymolysis of cellulose-containing raw material according to the method for embodiment 1, difference is:

[0126] In the premixing step of step (2), at 60° C., 4000 kilograms of steam-exploded cellulose-containing raw materials are continuously introduced into the premixing pipeline 11 of the premixing device 1 from the feed port 12 (feed rate is 320 kg / hours), the pH value regulator mass content is 40% sodium hydroxide solution, from the pH value regulator inlet 15 continuously introduced in the premixing pipeline 11, the screw feeder 14 is driven by the driving device, in the screw feeder 14 Under the agitation and extrusion of the spiral blade, the pH regulator is fully immersed in the steam-exploded cellulose-containing raw material, and stirred and mixed, and the inflow speed of the sodium hydroxide solution ...

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Abstract

The invention discloses an enzymolysis method for a cellulose-contained material. The method includes the step that the cellulose-contained material is subjected to enzymolysis to obtain an enzymolysis product, wherein before performing enzymolysis on the cellulose-contained material, a premixing step is also included. In the premixing step, under stirring and extruding conditions, the cellulose-contained material is mixed with a pH value modifier first to regulate the pH value of the cellulose-contained material to be 4-6, the cellulose-contained material with the pH value of 4-6 is mixed with an enzyme, and the dry matter content of the cellulose-contained material is 15-50% by weight. According to the method, the premixing step before the enzymolysis step is added, the pH value of the cellulose-contained material is regulated first, and then the cellulose-contained material is premixed with a part of the enzyme; in this way, the cellulose-contained material is under conditions for full enzymolysis before enzymolysis, that is, the pH value of the cellulose-contained material is more uniform, sufficient premixing between the cellulose-contained material and a part of the enzyme is realized, the utilization rate of the enzyme is effectively increased, and the enzyme mixing efficiency is enhanced.

Description

technical field [0001] The invention relates to an enzymatic hydrolysis method for cellulose-containing raw materials. Background technique [0002] The enzymatic hydrolysis process of cellulose-containing raw materials in the prior art usually adopts a stirring batch operation. In the presence of water, the cellulose-containing raw material and enzyme are added to the enzymolysis reactor at the same time, and the mixed reaction is adjusted to an appropriate pH value. , to maintain the reaction conditions for enzymatic hydrolysis to the end. Before enzymatic hydrolysis, the cellulose-containing raw material needs to be pretreated to expose the cellulose in the cellulose-containing raw material, so as to increase the contact area between the enzyme and the cellulose. [0003] The pretreatment of cellulose-containing raw materials by the method of the prior art will bring a series of problems to the subsequent enzymatic hydrolysis process, for example, adding the pretreated m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02C12M1/00C12M1/02
Inventor 林鑫武国庆熊强林海龙李凡沈乃东许克家高尚袁敬伟李春玲刘文信
Owner COFCO NUTRITION & HEALTH RES INST
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