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Marinobacter adhaerens for efficiently degrading agar polysaccharide

A high-efficiency, agar-based technology, applied in the field of microorganisms, can solve the problems of limitations, difficult separation, and low production of oligosaccharides

Active Publication Date: 2016-05-18
TIANJIN UNIV OF SCI & TECH
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Problems solved by technology

In the preparation of seaweed oligosaccharides, the specific enzymatic degradation preparation method of agar oligosaccharides has become the main research direction in recent years due to its advantages of high efficiency, strong specificity, mild reaction conditions and short action time. Agarase still has problems such as low yield, difficult separation, and low production of oligosaccharides, which limit its application in industrial production.

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  • Marinobacter adhaerens for efficiently degrading agar polysaccharide
  • Marinobacter adhaerens for efficiently degrading agar polysaccharide
  • Marinobacter adhaerens for efficiently degrading agar polysaccharide

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Embodiment Construction

[0026] The technical solutions of the present invention will be further described below in conjunction with specific embodiments.

[0027] 1. Screening of marine bacillus strain G4 (Marinobacteradhaerens):

[0028] (1) Enrichment culture: Select large agar-rich wild seaweed algae such as Sargassum, Geliflower or Gracilaria as the screening source, grind and pulverize 10g of seaweed samples and add them to 100mL enrichment medium, at 30°C Static culture for 1 week;

[0029] (2) Acclimatization culture: Take 10 mL of the enriched cultured bacterial solution, inoculate it into 100 mL of the acclimatization medium, and culture with shaking at 30°C for at least 48 hours;

[0030] (3) Separation and purification: spread the acclimatized and cultivated bacterial solution on the 2216E solid medium according to the dilution coating plate method, and select a single colony that forms an obvious dissolution zone on the agar plate after culturing at 30°C for 48 hours. Isolation method R...

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Abstract

The invention discloses Marinobacter adhaerens G4 with the preservation number of CGMCC No. 11016. The strain has the capacity of stably producing beta-agarase at high yield and can efficiently degrade agar polysaccharide to generate new agar tetrose. In a solution of the strain G4 domesticated and cultured for 48 hours, the enzyme activity of supernate can reach 323.48 U / mL. Meanwhile, the invention provides a method for preparing beta-agarase crude enzymes through the Marinobacter adhaerens G4. The method has the advantages of being rapid and easy and convenient to implement. In addition, the invention further provides a method for preparing the new agar tetrose from the beta-agarase crude enzymes produced by the Marinobacter adhaerens G4.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a bacterium of the genus Marine Bacillus capable of efficiently degrading agar polysaccharide. Background technique [0002] Agar gum is the main component of large economical red algae such as Gracilaria and Geliflower, and is widely used in biochemical, clinical, pharmaceutical and food fields. However, the characteristic of agar (polysaccharide) requiring specific enzymes for degradation has become a bottleneck hindering the further high-value and energy utilization of large economical seaweeds. [0003] Agarase is a class of enzymes that can specifically degrade agar polysaccharides. The agarases that have been discovered so far are mainly divided into α-agarase (the action site is α-1,3 glycosidic bond) and β-agarase (the site of action is β-1,4 glycosidic bond). According to the results of agarase sequence and functional analysis, it can be divided into six types of glycosid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/38C12P19/14C12R1/01
Inventor 朱大玲唐啸龙孙丽丽张宝玉王广策
Owner TIANJIN UNIV OF SCI & TECH
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