Construction method of RNA methylation sequencing library
A construction method and technology for sequencing libraries, applied in chemical libraries, combinatorial chemistry, library creation, etc., can solve the problem of high library noise
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[0035] It should be noted that, in the case of no conflict, the embodiments in the present application and the features in the embodiments can be combined with each other. The present invention will be described in detail below in conjunction with examples.
[0036] As mentioned in the background technology section, the immunoprecipitation-based RNA methylation library construction method in the prior art has the defect of high noise resulting in relatively low library quality. In order to improve this defect, in a typical implementation of the present invention In the method, a method for constructing an RNA methylation sequencing library is provided, the construction method comprising: step S1, fragmenting an RNA sample to obtain fragmented RNA; step S2, using an RNA methylation-specific antibody to fragment Co-immunoprecipitating the RNA to obtain a precipitate; Step S3, using protease to digest the precipitate to obtain a digest; Step S4, extracting RNA from the digest and...
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