Antibody composition and application of antibody composition in leukemia and lymphoma typing

An antibody composition and composition technology, applied in the direction of analytical materials, measuring devices, instruments, etc., can solve the problems of promotion restrictions, increase the difficulty of operation, increase the cost of typing, etc., and achieve broad application prospects, simple operation, and cost saving Effect

Active Publication Date: 2016-05-25
ZHEJIANG BOZHEN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This not only increases the cost of typing, but also increases the difficulty of operation, so the promotion is limited

Method used

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  • Antibody composition and application of antibody composition in leukemia and lymphoma typing
  • Antibody composition and application of antibody composition in leukemia and lymphoma typing
  • Antibody composition and application of antibody composition in leukemia and lymphoma typing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 reagent

[0041] In the following examples, monoclonal antibodies against the following two groups of cell surface antigens are used, and different monoclonal antibodies in each group are labeled with different fluorescent labels selected from FITC, PE, PerCP-cy5.5 and APC:

[0042] Group 1: CD45, CD13, CD33 and CD7;

[0043] Group 2: CD45, CD117, CD34 and CD19.

[0044] These fluorescently labeled monoclonal antibodies can be directly purchased through public channels, and the specific sources are shown in Table 1.

[0045] Table 1 Product-related monoclonal antibody information

[0046] raw material

Antibody clone number

manufacturer

supplier

APC-CD7 monoclonal antibody

CD7-6B7

American Biolegend

Dakowei Biotechnology Co., Ltd.

PE-CD13 Monoclonal Antibody

WM15

American Biolegend

Dakowei Biotechnology Co., Ltd.

APC-CD19 monoclonal antibody

HIB19

American Bioleg...

Embodiment 2

[0051] The processing of embodiment 2 sample

[0052] Adjust the bone marrow or peripheral blood samples stored in heparin or EDTA anticoagulant tubes to a cell density of 1-5×10 6 / ml, and then filtered through a 200-mesh industrial sieve to remove agglomerate-like substances in the sample to ensure a single cell suspension state, and store at 2-8°C to obtain the processed sample.

Embodiment 3

[0053] The typing detection of embodiment 3 samples

[0054] Take two flow tubes, mark ① and ② respectively, and then add the antibody mixture in tubes A and B of the kit in Example 1, respectively, in an amount of 3 μL. Then, take 100 μL of the sample treated in Example 2, add 50 μL to the flow tubes ① and ② after aliquoting, shake and mix with a vortex shaker, and incubate at room temperature (25° C.) in the dark for 15 minutes.

[0055] Dilute 10× PBS buffer solution to 1× PBS buffer solution with distilled water, then dilute 10× red blood cell lysate solution into 1× red blood cell lysate solution with 1× PBS buffer solution, and then pour into flow tubes ① and ② after incubation. Add 1ml of 1× red blood cell lysate, oscillate evenly with a vortex shaker, and let stand at room temperature (25°C) in the dark for 10 minutes.

[0056] Then, centrifuge flow tubes ① and ② at 1500rpm for 5 minutes, remove the supernatant, add 200 μL 1×PBS to resuspend, and use four-color flow c...

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PUM

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Abstract

The invention belongs to the technical field of antibody medicines and provides two types of antibody compositions including CD45 antibody, CD13 antibody, CD33 antibody and CD7 antibody or including CD45 antibody, CD117 antibody, CD34 antibody and CD19 antibody. The invention further provides a leukemia and lymphoma typing kit comprising the two types of antibody compositions and capable of being used with a flow cytometry to achieve typing of various hematological neoplasms.

Description

technical field [0001] The invention belongs to the technical field of antibody medicine, specifically, the invention relates to an antibody composition and its application in leukemia and lymphoma typing, a typing kit and the like. Background technique [0002] Common blood cancers mainly include various types of leukemia, myelodysplastic syndrome, multiple myeloma, and malignant lymphoma, which are major diseases that seriously endanger people's lives and health. Typing is often required after a blood disorder has been diagnosed diagnostically. For example, Chinese Patent No. 00133797 discloses a protein chip that can be used for leukemia typing, which includes antibodies against at least 19 cell surface antigens; Chinese Patent No. 200910082657 discloses a gene chip that can be used for acute lymphoblastic leukemia genotyping, It includes 62 kinds of DNA fragments; Chinese Patent No. 201410250033 discloses a kit for genotyping acute lymphoblastic leukemia, which includes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/574
CPCG01N33/533G01N33/574
Inventor 倪万茂
Owner ZHEJIANG BOZHEN BIOTECH CO LTD
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