Tissue culture rapid propagation method for coix lacryma-jobi
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A technology of tissue culture and Coix coix, which is applied in the field of plant cultivation, can solve the problems of low callus induction differentiation efficiency, single variety, and restricted development, and achieve the effect of high callus induction differentiation efficiency and good effect
Inactive Publication Date: 2016-06-01
SUBTROPICAL CROPS INST OF GUIZHOU PROVINCE
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Problems solved by technology
At present, the main problems in the development of Coix coix industry are single species, serious diseases, low yield, etc., which seriously restrict its development; the common way to solve the above problems in food crops is to introduce good genes into its agronomic traits to make them The traits have been effectively improved, but the success of genetic transformation requires the establishment of an efficient regeneration system, and plant tissue culture is a basic and important link in genetic transformation. Therefore, the establishment of an efficient regeneration genetic transformation system needs to be completed with the help of plant tissue culture technology.
[0004] In recent years, Coix tissue culture technology is mainly concerned with the induction and redifferentiation of callus and the culture of anther group. The techniques in Coix tissue culture technology, such as low callus induction differentiation efficiency and poor seedling induction effect, need to be further optimized.
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Embodiment 1
[0012] Embodiment 1: carry out according to the following steps:
[0013] 1. Select coix seeds as explants and carry out disinfection treatment. The disinfection treatment is soaking in 75% alcohol for 1 minute, washing with sterile water twice, adding 0.1% mercury liter for 15 minutes, and washing with sterile water for 3 times.
[0014] 2. Put the sterilized Coix explants into the Coix callus differentiation medium for culture and differentiation. The Coix coix callus differentiation medium is MS+2,4-D3.0mg / L+6-BA1.0mg / L .
[0015] 3. By cultivating the coix callus, strengthen the subculture of the coix callus at the same time, the adopted subculture medium of the coix coix callus is MS+2, 4D2.0mg / L+6-BA0.5mg / L.
[0016] 4. The best culture for the redifferentiation of Coix by subculture of Coix callus, the adopted Coix redifferentiation medium is MS+6-BA4.0mg / L+2,4D0.4mg / L.
[0017] 5. Through the re-differentiation of coix coix, when coix coix can reach seedling growth, ...
Embodiment 2
[0019] Embodiment 2: carry out according to the following steps:
[0020] 1. Select coix seeds as explants and carry out disinfection treatment. The disinfection treatment is to soak in 75% alcohol for 1 minute, rinse with sterile water for 3 times, add 10% sodium hypochlorite to soak for 20 minutes, and rinse with sterile water for 5 times.
[0021] 2. Put the sterilized Coix explants into the Coix callus differentiation medium for culture and differentiation. The Coix coix callus differentiation medium is MS+2,4-D3.0mg / L+6-BA1.0mg / L .
[0022] 3. By cultivating the coix callus, strengthen the subculture of the coix callus at the same time, the adopted subculture medium of the coix coix callus is MS+2, 4D2.0mg / L+6-BA0.5mg / L.
[0023] 4. The redifferentiation culture of Coix is carried out by subculture of Coix callus, and the adopted Coix redifferentiation medium is MS+6-BA4.0mg / L+2,4D0.4mg / L.
[0024] 5. Through the re-differentiation of coix coix, when coix coix can rea...
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Abstract
The invention discloses a tissue culture rapid propagation method for coix lacryma-jobi. The method comprises the following steps: selecting a coix lacryma-jobi explant, performing sterilization treatment on the coix lacryma-jobi explant, and placing the coix lacryma-jobi explant which is subjected to the sterilization treatment in a coix lacryma-jobi callus differentiation culture medium for culturing and differentiating; after coix lacryma-jobi calluses are cultured, strengthening coix lacryma-jobi callus subculture through a coix lacryma-jobi callus subculture medium; performing coix lacryma-jobi redifferentiation culture through a coix lacryma-jobi redifferentiation culture medium; culturing by adopting a coix lacryma-jobi seedling culture medium when the coix lacryma-jobi grows into a seedling through coix lacryma-jobi redifferentiation; after the coix lacryma-jobi grows into the seedling, performing coix lacryma-jobi rooting culture through a coix lacryma-jobi rooting culture medium. The tissue culture rapid propagation method for the coix lacryma-jobi disclosed by the invention is high in callus induction differentiation efficiency, and good in induction seedling effect; coix lacryma-jobi planting resources can be preserved, a genetic transformation system can be established, and the production of a virus-free tissue culture seedling can be realized; therefore, the tissue culture rapid propagation method for the coix lacryma-jobi is worthy of being popularized.
Description
technical field [0001] The invention relates to the rapid propagation technology of coix coix tissue culture and belongs to the technical field of plant cultivation. Background technique [0002] Coix is a crop of the genus Coix (coix.L.) in the grass family. Its seeds (commonly known as Coix) are rich in nutrients and can be used for food and medicine. and Job's tears. At present, the main problems in the development of Coix coix industry are single species, serious diseases, low yield, etc., which seriously restrict its development; the common way to solve the above problems in food crops is to introduce good genes into its agronomic traits to make them The traits are effectively improved, but the success of genetic transformation requires the establishment of an efficient regeneration system, and plant tissue culture is a basic and important link in genetic transformation. Therefore, the establishment of an efficient regeneration genetic transformation system needs to ...
Claims
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Application Information
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