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Method for sweet potato stem tip in vitro rapid propagation

A stem tip and sweet potato technology, which is applied in the field of in vitro rapid propagation of sweet potato stem tips, can solve problems such as yield decline and quality degradation of sweet potato vegetative propagation, and achieve the effect of improving quality and yield and having great application value

Inactive Publication Date: 2016-06-01
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a method for quick propagation of sweet potato shoot tips in vitro. Through tissue culture technology, sweet potato shoot tips are selected, sterilized, inoculated into culture media with different hormones and concentrations for cultivation, and the best ones are screened out. A system for quickly obtaining virus-free seedlings to solve the problems of long-term asexual reproduction quality degradation and yield decline of sweet potatoes

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  • Method for sweet potato stem tip in vitro rapid propagation
  • Method for sweet potato stem tip in vitro rapid propagation
  • Method for sweet potato stem tip in vitro rapid propagation

Examples

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Embodiment Construction

[0022] The methods used in the following examples are conventional methods unless otherwise specified. MS medium, sucrose, agar, and hormone KT (kinetin) were all produced by Sulaibao Company, 1 / 2MS medium was produced by Shanghai Lichen Trading Co., Ltd., alcohol was produced by Nanjing Chemical Reagent Co., Ltd., polyvinylpyrrolidone K- 30 (PVP) was produced by Sinopharm Chemical Reagent Co., Ltd. Hormone NAA (a-naphthaleneacetic acid) and IBA (3-indolebutyric acid) were produced by Shanghai Kaiyang Biotechnology Co., Ltd. Hormone 6-BA (6-benzylamino Adenine) was purchased from Shanghai Ruiyong Biotechnology Co., Ltd., and sweet potatoes were grown in Nanjing Zhongshan Botanical Garden in the experiment. The cultivation process is described in detail below.

[0023] 1. Sampling

[0024] From the sweet potato introduced in Nanjing Zhongshan Botanical Garden, take the young shoot tip of a plant that grows strong and has no damage from diseases and insect pests, rinses it wit...

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Abstract

The invention discloses a method for sweet potato stem tip in vitro rapid propagation. Through in vitro culture of mature and delicate stem tips of sweet potatoes, the optional experimental conditions are screened, the optional rapid propagation system for sweet potato culture is established, and the fact that the optimal hormone concentration in budding inducing culture medium is NAA 0.1 mg / L plus KT 2.0 mg / L, the optimal hormone concentration in subculture medium is NAA 0.5 mg / L plus KT 2.0mg / L, and the optimal hormone concentration for rooting culture is IBA 2.0mg / L can be obtained. The method can rapidly obtain virus-free seedlings of sweet potatoes, improves the quality and yield of sweet potatoes, and provides a theoretical basis and practice guidance for the industrialization production of sweet potatoes.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and relates to a method for quick propagation of sweet potato stem tips in vitro. Background technique [0002] Sweet potato (D.esculenta (Lout) brukill) is a different kind of edible plant belonging to yam. The top grade is a nourishing food, containing polysaccharides, starch, protein, free amino acids and trace elements, etc. It has the functions of immune regulation, anti-oxidation, lowering blood sugar, lowering blood lipids, anti-tumor, anti-mutation, and regulating spleen and stomach functions. It is a famous Chinese food. The tonic Chinese medicine is also a traditional health care dish. The edible part of the sweet potato is a spherical tuber similar to a potato. The meat is tender, delicious, and soft in the mouth. It can be used as vegetables, miscellaneous grains or medicinals. [0003] At present, sweet potato is a small farm species with rich varieties but no large-scale cult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 孙小芹李定红杭悦宇
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI