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Nostoc sphaeroides polysaccharide and extracting method thereof

An extraction method, the technology of kudzu rice flour, applied in the chemical industry, can solve the problems of high cost, many processes, energy consumption and time consumption, and achieve the effect of reducing extraction cost, improving preparation efficiency and good appearance

Inactive Publication Date: 2016-06-01
HUNAN YANDI BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the traditional extraction method of kudzu rice polysaccharide consumes energy and time, has many processes, and uses a large amount of ethanol for alcohol precipitation, which is costly

Method used

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  • Nostoc sphaeroides polysaccharide and extracting method thereof
  • Nostoc sphaeroides polysaccharide and extracting method thereof
  • Nostoc sphaeroides polysaccharide and extracting method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Step 1: Preparation of materials: Select kudzu rice and dry it at 60° C. for 2 hours; grind the dried kudzu rice and pass through a 80-mesh sieve to obtain kudzu rice flour.

[0048] Step 2: Soaking: Soak the kudzu rice noodles with purified water, the weight ratio of kudzu rice noodles to purified water is 1:30, and the soaking time is 24 hours, so as to obtain the soaked kudzu rice.

[0049] Step 3: water extraction: add purified water to the soaked kudzu rice, the ratio of solid to liquid is 1:15 (purified water with 15 times the quality of kudzu rice after water absorption is added), the extraction temperature is 90°C, and the extraction time After 2 hours, the feed liquid was cooled to room temperature and centrifuged to discard the feed slag, and the feed liquid was collected to obtain an extract.

[0050] Step 4: Enzymolysis: the extract is heated to 55°C, and the papain (10% of the volume of the extract) is added to the extract. 4 u / g) (w / v), enzymatic hydrolys...

Embodiment 2

[0056] Step 1: Preparation of materials: Select kudzu rice and dry it at 80° C. for 1 hour; grind the dried kudzu rice and pass through a 50-mesh sieve to obtain kudzu rice flour.

[0057] Step 2: Soaking: Soak the kudzu rice noodles with purified water, the weight ratio of kudzu rice noodles to purified water is 1:10, and the soaking time is 2 hours, so as to obtain the soaked kudzu rice.

[0058] Step 3: water extraction: add purified water to the soaked kudzu rice, the ratio of solid to liquid is 1:8 (purified water with 8 times the quality of kudzu rice after water absorption is added), the extraction temperature is 100°C, and the extraction time is After 1 hour, the feed liquid was cooled to room temperature and centrifuged to discard the feed slag, and the feed liquid was collected to obtain an extract.

[0059] Step 4: Enzymolysis: the extract is heated to 40°C, and the papain (10% of the volume of the extract) is added to the extract. 4 u / g) (w / v), enzymatic hydrolysi...

Embodiment 3

[0065] Step 1: Preparation of raw materials: Select kudzu rice and dry it at 50° C. for 5 hours; grind the dried kudzu rice and pass through a 100-mesh sieve to obtain kudzu rice flour.

[0066] Step 2: Soaking: Soak the kudzu rice noodles with purified water, the weight ratio of kudzu rice noodles to purified water is 1:80, and the soaking time is 24 hours, so as to obtain the soaked kudzu rice.

[0067] Step 3: water extraction: add purified water to the soaked Gexian rice, the ratio of solid to liquid is 1:20 (adding purified water 20 times the quality of Ge Xianmi after water absorption), the extraction temperature is 80°C, and the extraction time is 1:20. After 5 hours, the feed liquid was cooled to room temperature and centrifuged to discard the feed slag, and the feed liquid was collected to obtain an extract.

[0068] Step 4: Enzymolysis: the extract is heated to 70°C, and the papain (10% of the volume of the extract) is added to the extract. 4 u / g) (w / v), enzymatic h...

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Abstract

The invention relates to the technical field of chemical engineering, in particular to a nostoc sphaeroides polysaccharide and an extracting method thereof. According to the nostoc sphaeroides polysaccharide and the extracting method thereof, the nostoc sphaeroides polysaccharide is extracted through the processing steps of material preparing, soaking, water extracting, enzymolysis performing, ultrafiltering, concentrating, sterilizing and drying, and nostoc sphaeroides polysaccharide extraction can be achieved without needing alcohol precipitation processing in the processing process. Therefore, not only can the purity of the nostoc sphaeroides polysaccharide be effectively improved, but also the preparation efficiency of the nostoc sphaeroides polysaccharide can be effectively improved and the extraction cost can be effectively lowered; the extraction method is a green production technology suitable for industrialized production, the nostoc sphaeroides polysaccharide extracted through the method has the advantages of being high in purity and excellent in appearance, and the molecular weight specification of the nostoc sphaeroides polysaccharide can be selected.

Description

technical field [0001] The invention relates to the technical field of chemical engineering, in particular to a kudzu rice polysaccharide and an extraction method thereof. Background technique [0002] Gexianmi is the common name of Nostocsphaeoides Kützing in NostocVauch, which belongs to Cyanophyta, Cyanophyceae, Nostocales, and Nostocaceae. Also known as tianxian rice, tianxiancai, pearl vegetable, water fungus, and field fungus, it is a precious wild freshwater nitrogen-fixing cyanobacteria traditionally exported for food and medicine in my country. [0003] Gexianmi has a long history in my country, and it is not only a nutritious traditional delicacy, but also has high medical and health care effects. According to records, Gexianmi has the effects of treating night blindness, rectal prolapse, burns and beauty. In addition, it also has the effects of improving eyesight and Qi, relieving heat and clearing diaphragm, benefiting stomach, long-term food and prolonging life...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/0084
Inventor 王玉兰陈盛赵立彬牛新明
Owner HUNAN YANDI BIOLOGICAL ENG
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