Method for preparing (S)-3-hydroxytetrahydrofuran based on erythritol microorganisms
A hydroxytetrahydrofuran and microorganism technology, which is applied to microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of not many choices, and achieve the effects of simplified steps, low cost, and mild reaction.
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Embodiment 1
[0023] (1) Taking the glucose solution with a concentration of 30% as raw material, taking Candida lipolytica as the fermentation strain, and assisting with trace elements, carry out expanded cultivation on a shaking table, then ferment for 3 days at 25°C, and obtain a fermentation inoculum size of 5 % of the erythritol crude product, the erythritol crude product is decolorized by activated carbon, filtered through a membrane, and separated by a macroporous resin to obtain an erythritol solution with a content of 80%.
[0024] (2) According to the dosage ratio, 1 part of erythritol solution prepared in step (1) is placed in a three-necked flask as a raw material, and 4 parts of hydrogen gas is introduced at a gas flow rate of 0.3 L / min, and citric acid solution is added. Adjust the pH value of the system to 5, stir and react at 45°C for 8 hours, then add an alkaline solution to neutralize to neutralize the reaction to stop, finally extract, and distill under reduced pressure to...
Embodiment 2
[0026] (1) With the concentration of 50% glucose solution as raw material, with Candida lipolytica as the fermentation strain, and with the assistance of trace elements, expand the culture on a shaking table, then ferment for 5 days at 35°C, and obtain a fermentation inoculum size of 10 % of the erythritol crude product, the erythritol crude product is decolorized by active carbon, membrane filtration, macroporous resin separation to obtain a content of 92% erythritol solution.
[0027] (2) According to the dosage ratio, 1 part of erythritol solution prepared in step (1) is placed in a three-necked flask as a raw material, and 6 parts of hydrogen gas is introduced at a gas flow rate of 0.5 L / min, and hydrochloric acid aqueous solution is added to adjust The pH value of the system was 6, and the reaction was stirred at 55°C for 12 hours, then an alkaline solution was added to neutralize to neutralize to stop the reaction, and finally extracted and distilled under reduced pressur...
Embodiment 3
[0029] (1) With the concentration of 40% glucose solution as raw material, with Candida lipolytica as the fermentation strain, and with the assistance of trace elements, expand the culture on a shaking table, then ferment for 4 days at 30°C, and obtain a fermentation inoculum size of 7 % of the erythritol crude product, the erythritol crude product is decolorized by active carbon, membrane filtration, macroporous resin separation to obtain a content of 84% erythritol solution.
[0030] (2) According to the dosage ratio, 1 part of erythritol solution prepared in step (1) is placed in a three-necked flask as a raw material, and 5 parts of hydrogen are fed into 5 parts of hydrogen at a gas flow rate of 0.4L / min, and hydrochloric acid aqueous solution is added to adjust The pH value of the system was 5.1, stirred and reacted at 50°C for 10 h, then neutralized to neutral by adding an alkaline solution to stop the reaction, finally extracted, and distilled under reduced pressure to o...
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