Method for preparing bovine cornea stroma from fresh bovine cornea and application method

Abstract

The invention provides a method for preparing a bovine cornea stroma from a fresh bovine cornea. The method comprises the steps that a cornea piece is cut from a fresh bovine eyeball and then steeped in a hypotonic solution; the bovine cornea stroma containing a lamina elastica anterior and part of a stroma layer is obtained from the cornea piece, and is frozen and thawed repeatedly to break cells; the bovine cornea stroma is bleached with a buffer solution, steeped in a cell removing reagent and then washed with ultrapure water in sequence; the bovine cornea stroma is put into another cell removing reagent for steeping, and then washed with ultrapure water again; the bovine cornea stroma is put into a dehydrating agent to be steeped and then irradiated with cobalt-60, and then the prepared bovine cornea stroma is dried and stored. The physical method and low-toxicity regents are adopted in the method, and time is very short, so that the obtained bovine cornea stroma is completely preserved, has the advantages of being good in transparence and compact in structure, and is close to the natural feature of the human cornea.

Description

technical field [0001] The invention relates to a method for preparing bovine corneal stroma, in particular to a method for preparing bovine corneal stroma by utilizing fresh bovine cornea and an application method. Background technique [0002] Corneal disease is the fourth leading cause of blindness in the world. According to the World Health Organization, corneal disease is the fourth leading cause of blindness after cataracts, glaucoma, and age-related macular degeneration, accounting for about 5.1%. The 2006 "Second National Sampling Survey of Disabled People Statistical Results" showed that there were about 4 million blind patients due to corneal diseases, and more than 100,000 new patients were added every year, of which about 2 million could recover their eyesight through corneal transplantation. The number of corneal transplants in China is 4,000-5,000, and a large number of patients are blind due to lack of donor sources. About 3 million patients are "waiting for t...

AI Technical Summary

Problems solved by technology

The 2006 "Second National Sampling Survey of Disabled Persons" showed that there were about 4 million blind patients due to corneal diseases, and more than 100,000 new patients were added every year, of which about 2 million could recover their eyesight through corneal transplantation, but every year The number of corneal transplants in China is 4,000-5,000. A large number of patients are blind due to lack of donor sources. About 3 million patients are "waiting for the rice to cook." The provision of not allowing the use of the remains of executed prisoners has greatly reduced the supply of corneas. The number of patients waiting in line for corneal donors will increase significantly each year

[0003] In terms of corneal carrier scaffolds, although domestic and foreign scholars have conducted a large number of experimental studies on natural biomaterials and composite materials such as amniotic membrane and heterogeneous decellularized porcine corneal stroma, the carrier scaffolds that can really meet the clinical requirements are still blank, and the reported heterogeneous ( For example, the scaffold formed after decellularization treatment also has fatal defects such as severe structural damage, low transparency, and poor elasticity.

In addition, detergents such as pancreatin, ethylenediaminetetraacetic acid solution, polyethylene glycol octylphenyl ether, and sodium lauryl sulfate are used in the process of preparing decellularized bovine corneal stroma to inhibit natural organisms such as collagen in the stroma. The membrane made of macromolecules has a great destructive effect, and the obvious opacity of the cornea after transplantation in clinical reports also confirms the defects of this material and cannot be used clinically.

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