Method for promoting haematococcus pluvialis to produce astaxanthin by utilizing butylated hydroxyanisole

A technology of butylated hydroxy fennel and Haematococcus pluvialis, applied in the field of bioengineering, can solve the problems of high equipment requirements, cumbersome operation, complicated process, etc., and achieves the effects of low cost, improved yield, and simple and easy operation.

Inactive Publication Date: 2016-06-08
KUNMING UNIV OF SCI & TECH
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Problems solved by technology

Although the above-mentioned relevant patented technologies are advanced, the process is relatively complicated, the equipment requiremen

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preparation example Construction

[0018] Step 1. Preparation of algae liquid: Haematococcus pluvialis strains are used to cultivate Haematococcus pluvialis strains, using BBM medium, at 25°C, light intensity 3000lx, and continuous light conditions to cultivate Haematococcus pluvialis to achieve logarithmic growth At the end of the period, the concentration of algae cells reached 10 6 cellsmL -1 , centrifuged under sterile conditions, and diluted to 2×10 with nitrogen-deficient BBM medium 5 cellsmL -1 As an induced algae solution;

[0019] Step 2. Inducing Haematococcus pluvialis to accumulate astaxanthin: make 128gL with DMSO -1 The butylated hydroxyanisole mother liquor, the butylated hydroxyanisole mother liquor is added to the diluted algae induction liquid to make the butylated hydroxyanisole concentration reach 2-8mgL -1 Then, the above-mentioned algae liquid is cultivated under the combined stress of 28° C., light intensity of 10000 lx, continuous light of cold light and nitrogen starvation, and the ...

Embodiment 1

[0023] (1) Haematococcus pluvialls adopts the strain of Haematococcus pluvialls, utilizes BBM culture medium, cultivates under 25 ℃, light intensity 3000lx, continuous light conditions, cultivates Haematococcus pluvialls to reach logarithmic growth phase, at this time algae Cell concentration up to 10 6 cellsmL -1 , centrifuged under sterile conditions, and diluted to 2×10 with nitrogen-deficient BBM medium 5 cellsmL -1 As an inducing algae fluid.

[0024] (2) Make 128gL with DMSO -1 Butylated hydroxyanisole mother liquor, the butylated hydroxyanisole mother liquor is added to the diluted algae induction liquid to make the butylated hydroxyanisole concentration reach 2mgL -1 Then the above-mentioned algae liquid was cultivated under the compound stress of 28°C, light intensity 10000lx, cold light continuous light and starvation nutrient salts, and the color changes of algae cells were observed regularly by sampling every day. After 13 days, the algae cells were found to be...

Embodiment 2

[0026] (1) Haematococcus pluvialls adopts the strain of Haematococcus pluvialls, utilizes BBM medium, cultivates under 25 ℃, light intensity 3000lx, continuous light conditions, cultivates until Haematococcus pluvialis reaches the logarithmic growth phase, at this time Algae cell concentration reaches 10 6 cellsmL -1 , diluted to 2×10 with nitrogen-deficient BBM medium 5 cellsmL -1 As an inducing algae fluid.

[0027] (2) Make 128gL with DMSO -1 The butylated hydroxyanisole mother liquor, the butylated hydroxyanisole mother liquor is added to the diluted algae induction liquid to make the butylated hydroxyanisole concentration reach 4mgL -1 Then the above-mentioned algae liquid was cultivated under the compound stress of 28°C, light intensity 10000lx, cold light continuous light and starvation nutrient salt, and the color change of algae cells was observed regularly by sampling every day. Cells use organic solvents to extract astaxanthin from algal cells, and the accumula...

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Abstract

The invention discloses a method for promoting haematococcus pluvialis to produce astaxanthin by utilizing butylated hydroxyanisole. Firstly, a haematococcus pluvialis solution is prepared, in the later period of the logarithmic phase of haematococcus pluvialis, the cell concentration reaches 10<6> cells mL<-1>, the solution is diluted with a nitrogen-deficient BBM culture medium until the concentration of the solution reaches 2*10<5> cells mL<-1>, and the solution for induction is obtained; then, a butylated hydroxyanisole mother solution with the concentration being 128 g L<-1> is prepared with DMSO (dimethyl sulfoxide) and added to the diluted induction solution, and accordingly, the concentration of butylated hydroxyanisole reaches 4.95-5.05 mg L<-1>; the solution is cultured, after cells of algae completely turn red, the cells of algae are collected, and at the moment, the astaxanthin accumulation quantity reaches the maximum. The method is simple and feasible, the cost is low, the time consumed when haematococcus pluvialis turns red is greatly shortened, the quantity of astaxanthin is remarkably increased, and the yield of astaxanthin is greatly increased.

Description

technical field [0001] The invention specifically relates to a method for promoting the production of astaxanthin by Haematococcus pluvialls by using butyl hydroxyanisole, which belongs to the technical field of bioengineering. Background technique [0002] Natural astaxanthin (3,3′-dihydroxy-4,4′-diketo-β,β′-carotene) is the highest derivative of carotenoids, and its specific structure determines its antioxidant activity significantly higher than other carotenoids. Astaxanthin, as a strong antioxidant, has great applications in the fields of medicine, cosmetics and health products, and is also widely used as an additive in food, aquatic products and other industries. [0003] Natural astaxanthin mainly comes from Phaffia yeast, salmon, shrimp shells and Haematococcus pluvialis, among which Haematococcus pluvialis can accumulate a large amount of astaxanthin under stress conditions, such as high temperature, strong light, nitrogen starvation, etc. , its content can reach 5...

Claims

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Application Information

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IPC IPC(8): C12P23/00C07C403/24C12R1/89
CPCC07C403/24C12P23/00
Inventor 余旭亚尚敏敏徐军伟李涛赵鹏
Owner KUNMING UNIV OF SCI & TECH
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