Method for promoting haematococcus pluvialis to produce astaxanthin by utilizing butylated hydroxyanisole

Abstract

The invention discloses a method for promoting haematococcus pluvialis to produce astaxanthin by utilizing butylated hydroxyanisole. Firstly, a haematococcus pluvialis solution is prepared, in the later period of the logarithmic phase of haematococcus pluvialis, the cell concentration reaches 10<6> cells mL<-1>, the solution is diluted with a nitrogen-deficient BBM culture medium until the concentration of the solution reaches 2*10<5> cells mL<-1>, and the solution for induction is obtained; then, a butylated hydroxyanisole mother solution with the concentration being 128 g L<-1> is prepared with DMSO (dimethyl sulfoxide) and added to the diluted induction solution, and accordingly, the concentration of butylated hydroxyanisole reaches 4.95-5.05 mg L<-1>; the solution is cultured, after cells of algae completely turn red, the cells of algae are collected, and at the moment, the astaxanthin accumulation quantity reaches the maximum. The method is simple and feasible, the cost is low, the time consumed when haematococcus pluvialis turns red is greatly shortened, the quantity of astaxanthin is remarkably increased, and the yield of astaxanthin is greatly increased.

Description

technical field [0001] The invention specifically relates to a method for promoting the production of astaxanthin by Haematococcus pluvialls by using butyl hydroxyanisole, which belongs to the technical field of bioengineering. Background technique [0002] Natural astaxanthin (3,3′-dihydroxy-4,4′-diketo-β,β′-carotene) is the highest derivative of carotenoids, and its specific structure determines its antioxidant activity significantly higher than other carotenoids. Astaxanthin, as a strong antioxidant, has great applications in the fields of medicine, cosmetics and health products, and is also widely used as an additive in food, aquatic products and other industries. [0003] Natural astaxanthin mainly comes from Phaffia yeast, salmon, shrimp shells and Haematococcus pluvialis, among which Haematococcus pluvialis can accumulate a large amount of astaxanthin under stress conditions, such as high temperature, strong light, nitrogen starvation, etc. , its content can reach 5...

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Problems solved by technology

Although the above-mentioned relevant patented technologies are advanced, the process is relatively complicated, the equipment requirements are high, and the operation is relatively cumbersome, which increases the cost of production and the difficulty of technology promotion