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Bioactive preparation of specific anti-variola virus infection model strain and application of bioactive preparation

A technology of biological activity and preparation, applied in the direction of antiviral agents, viruses, microorganisms, etc., can solve the problems of large toxic and side effects, limited promotion and application, low biological utilization efficiency, etc., and achieve the effect of low toxicity and high absorption rate.

Active Publication Date: 2016-06-15
INST OF PLA FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the most researched substances with good inhibitory effects on vaccinia virus infection are mainly various nucleoside compounds and nucleoside analogs, including: inosine monophosphate (IMP) dehydrogenase inhibitors (such as Li Bavirin), S-adenosylcysteine ​​(SAH) hydrolase inhibitors (such as adenosine analog A), orotidine monophosphate (OMP) decarboxylase inhibitors (such as pyrazolin) , cytidine triphosphate (CTP) synthase inhibitors (such as cyclopentenylcytosine (Ce-Cyd)), acyclic nucleoside carbon phosphate compounds, such as cidofovir (cidofovir), but due to such anti Poxvirus compounds have low bioavailability, high toxicity and side effects, etc., which seriously limit their comprehensive promotion and application.

Method used

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  • Bioactive preparation of specific anti-variola virus infection model strain and application of bioactive preparation
  • Bioactive preparation of specific anti-variola virus infection model strain and application of bioactive preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1, preparation and component identification of bioactive preparation CH2009

[0041] 1. Preparation of biologically active preparation CH2009

[0042] 1. Take out the stimulating antigen (vaccinia virus) from the ultra-low temperature refrigerator at -80°C, place it in a water bath at 37°C and dissolve it quickly, add an appropriate amount of PBS, and prepare a titer of 1×10 6 TCID 50 / ml of virus fluid for inoculation.

[0043] 2. Pick healthy and mature New Zealand white rabbits, weighing about 2.7-3.0 kg, cut off the back hair, and wipe and disinfect the depilated parts with 75% alcohol cotton balls, inoculate the above virus liquid 0.05ml / inoculation point intradermally, and inoculate 100- 120 sites / rabbit.

[0044] 3. Feed 4-5 days after virus inoculation. New Zealand white rabbits with good pox symptoms, color change from ruddy to purple, skin thickening and subcutaneous edema were euthanized, and the skin was harvested within 15 minutes. The size of th...

Embodiment 2

[0059] Embodiment 2, the determination of the anti-pox virus activity of bioactive preparation CH2009

[0060] In this example, vaccinia virus and mousepox virus, which are recognized in the art as surrogate virus models of smallpox virus, will be used as the tested poxviruses to study the antiviral activity of the biologically active agent CH2009 against smallpox and other poxviruses from the cell and animal levels, respectively. .

[0061] 1. Determination of the activity of biologically active preparation CH2009 in inhibiting poxvirus infection in vitro

[0062] Tested viruses and corresponding sensitive cells: vaccinia virus WR strain (VacciniaVirusWRStrain), mousepox virus Moscow strain (Ectromelia Viruses Moscow strain, ECTV-MOSstrain), coronavirus (murine hepatitis coronavirus A59 strain, referred to as MHV-A59), adenovirus type 5 (dl309 strain), enterovirus 71 type Hn2 strain (EV71-Hn2). The sensitive cells of vaccinia virus and mousepox virus are CV-1 cells; the sen...

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Abstract

The invention discloses a bioactive preparation of a specific anti-variola virus infection model strain and application of the bioactive preparation. The bioactive preparation CH2009 is specifically applied to (1) preparation of products for inhibiting poxvirus infection or (2) inhibition of poxvirus infection. The bioactive preparation CH2009 with a specific anti-poxvirus infection function is prepared from inflammatory skin tissues of a New Zealand white rabbit through the steps of high temperature and high pressure treatment, solvent extraction, acid and alkali treatment, adsorption, elution, centrifugal concentration and the like by utilizing intracutaneous inoculation of poxviruses on the New Zealand white rabbit. The invention provides a brand-new design strategy for research and development of new-generation drugs for resisting infection of poxviruses, such as variola; and the obtained antiviral drug certainly has wide application prospects and huge commercial values.

Description

technical field [0001] The invention belongs to the field of antiviral drugs, and relates to a bioactive preparation and application of a specific anti-smallpox virus infection model strain. Background technique [0002] Poxviruses are a large family that can infect both humans and animals. They often cause local or systemic purulent skin lesions in infected persons, and can lead to outbreaks of infectious diseases. Of particular note is the smallpox virus, which reproduces rapidly and can be transmitted through the air at an extremely rapid rate. Patients with the virus are most contagious within 1 week of infection because their saliva contains the largest amount of smallpox virus, until After the patient's scar is peeled off, the smallpox virus can also be transmitted through the patient, which has led to many global outbreaks in human history. In the case of malignant infection, the smallpox virus causes serious diffuse damage in the tissue layer or deep layer of the sk...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/36A61P31/14
CPCA61K35/36C12N2710/24134A61K39/12A61P31/20A61K2039/58A61K35/76A61K39/285A61K2039/552
Inventor 常国辉刘京梅罗保君张洁罗丽晓孙走南罗彦军杨益李瑾惠唐玥苏文莉
Owner INST OF PLA FOR DISEASE CONTROL & PREVENTION
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