Biologically active preparation and application of specific anti-variola virus infection model strain
一种生物活性、制剂的技术,应用在抗病毒剂、病毒、微生物等方向,能够解决毒副作用大、限制推广和应用、生物利用效率低等问题,达到毒性低、吸收率高的效果
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0040] Example 1, preparation and component identification of bioactive preparation CH2009
[0041] 1. Preparation of biologically active preparation CH2009
[0042] 1. Take out the stimulating antigen (vaccinia virus) from the ultra-low temperature refrigerator at -80°C, place it in a water bath at 37°C and dissolve it quickly, add an appropriate amount of PBS, and prepare a titer of 1×10 6 TCID 50 / ml of virus fluid for inoculation.
[0043] 2. Pick healthy and mature New Zealand white rabbits, weighing about 2.7-3.0 kg, cut off the back hair, and wipe and disinfect the depilated parts with 75% alcohol cotton balls, inoculate the above virus liquid 0.05ml / inoculation point intradermally, and inoculate 100- 120 sites / rabbit.
[0044] 3. Feed 4-5 days after virus inoculation. New Zealand white rabbits with good pox symptoms, color change from ruddy to purple, skin thickening and subcutaneous edema were euthanized, and the skin was harvested within 15 minutes. The size of th...
Embodiment 2
[0059] Embodiment 2, the determination of the anti-pox virus activity of bioactive preparation CH2009
[0060] In this example, vaccinia virus and mousepox virus, which are recognized in the art as surrogate virus models of smallpox virus, will be used as the tested poxviruses to study the antiviral activity of the biologically active agent CH2009 against smallpox and other poxviruses from the cell and animal levels, respectively. .
[0061] 1. Determination of the activity of biologically active preparation CH2009 in inhibiting poxvirus infection in vitro
[0062] Tested viruses and corresponding sensitive cells: vaccinia virus WR strain (Vaccinia Virus WR Strain), ratpox virus Moscow strain (Ectromelia Viruses Moscow strain, ECTV-MOS strain), coronavirus (murine hepatitis coronavirus A59 strain, referred to as MHV- A59), type 5 adenovirus (dl309 strain), enterovirus 71 type Hn2 strain (EV71-Hn2). The sensitive cells of vaccinia virus and mousepox virus are CV-1 cells; the ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com