Method for producing inonotus obliquus powder through rapid and deep liquid state fermentation

A technology of deep liquid fermentation and Inonotus obliquus, which is applied in the field of microorganisms, can solve the problems of large workload of sewage treatment system, decreased product quality and yield, large consumption of manpower and material resources, etc., so as to improve the utilization rate of equipment and clarify the filtrate. , The effect of easy maintenance of the device

Inactive Publication Date: 2016-06-15
JIANGSU SHENHUA PHARMA
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  • Abstract
  • Description
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Problems solved by technology

[0006] The traditional liquid fermentation process of Inonotus obliquus is mostly an intermittent batch fermentation process, but the batch fermentation process consumes a lot of manpower and material resources, and each batch of fermentation needs to be loaded, sterilized, inoculated, discharged, cleaned, etc. The operation is complicated, the process is cumbersome, the non-fermentation period is long, and the production efficiency is low; while the subsequent separation process is traditionally separated by a centrifuge. This method is subject to the influence of the

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  • Method for producing inonotus obliquus powder through rapid and deep liquid state fermentation
  • Method for producing inonotus obliquus powder through rapid and deep liquid state fermentation
  • Method for producing inonotus obliquus powder through rapid and deep liquid state fermentation

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Embodiment 1

[0029] 1) Inonotus obliquus strain (Inonotus obliquus) is preserved in the China Forestry Microorganism Culture Collection and Management Center (strain code cfcc60260). The cultured Inonotus obliquus strain seed solution is seeded into the seed medium, the inoculum size is 10%, and the seed medium is composed of glucose 2%, silkworm chrysalis powder 0.3%, soybean cake powder 1.8%, potassium dihydrogen phosphate 0.3%, Magnesium sulfate heptahydrate 0.15%, soybean oil 0.02%; adjust the pH value to 6.5. The cultivation conditions are: the ventilation ratio is 1:0.4vvm, the tank pressure is 0.03Mpa, the cultivation temperature is 26-28°C, and the seed liquid can be obtained after culturing for about 5 days. Inoculate the cultivated seed liquid into 50L fermentation medium after sterilization, the inoculation amount is 15%, and the fermentation medium is composed of 2.5% glucose, 0.5% silkworm chrysalis powder, 1.5% soybean cake powder, 0.3% potassium dihydrogen phosphate, and sul...

Embodiment 2

[0037] 1) Inonotus obliquus strain (Inonotus obliquus) is preserved in the China Forestry Microorganism Culture Collection and Management Center (strain code cfcc60260). Seed the cultured Inonotus obliquus bacterial strain seed solution to the seed medium, the inoculum size is 10%, and the seed medium consists of glucose 2.5%, silkworm chrysalis powder 0.4%, soybean cake powder 2.0%, potassium dihydrogen phosphate 0.3%, Magnesium sulfate heptahydrate 0.2%, soybean oil 0.02%; adjust the pH value to 6.5. The cultivation conditions are: the ventilation ratio is 1:0.4vvm, the tank pressure is 0.03Mpa, the cultivation temperature is 26-28°C, and the seed liquid can be obtained after culturing for about 5 days. Inoculate the cultivated seed liquid into 100L fermentation medium after sterilization, the inoculation amount is 15%, and the fermentation medium is composed of glucose 2.8%, silkworm chrysalis powder 0.6%, soybean cake powder 1.8%, potassium dihydrogen phosphate 0.3%, sulfu...

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Abstract

The invention discloses a method for producing Inonotus obliquus bacterial powder by rapid deep liquid fermentation. The method is to quickly obtain a fermented liquid rich in a large amount of mycelium by adopting a semi-continuous fermentation process for the Inonotus obliquus strain, and then The fermented liquid is treated and separated by a microfiltration membrane to obtain the mycelia of Inonotus obliquus. The invention changes the traditional intermittent batch fermentation process of Inonotus obliquus, and the separation of mycelium adopts efficient ceramic membrane microfiltration to replace the centrifuge separation mode. The semi-continuous fermentation process overcomes the shortcomings of the traditional batch fermentation process, such as many process links, long intermediate intervals, and low equipment efficiency; and the use of high-efficiency ceramic membranes to filter the fermentation liquid can reduce the mycelium entering the filtrate, which can not only improve the bacterial The yield of silk can reduce the COD of the filtrate, reduce the amount of sewage treatment, without destroying the cells themselves, and ensure the recovery of all active ingredients in the cells. Compared with the traditional technology, the invention has remarkable economic benefit, social benefit and environmental protection benefit.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for rapidly producing fungal mycelium by deep liquid fermentation technology, more precisely, a method for rapidly producing Inonotus obliquus powder by deep liquid fermentation. Background technique [0002] Microbial fermentation can be divided into batch, fed-batch, semi-continuous, continuous and other modes. Batch fermentation consumes a lot of manpower and material resources. Each batch of fermentation needs to be loaded, sterilized, inoculated, discharged, and cleaned. The process is cumbersome, the fermentation cycle is long, and the production efficiency is low; Fed-batch fermentation Although the lack of nutrients or precursors can be supplemented by feeding, the product synthesis will inevitably be blocked due to the continuous accumulation of harmful metabolites; the production intensity of continuous fermentation is greatly improved compared wit...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C12R1/645
CPCC12N1/14C12N1/02
Inventor 王文风徐国华徐玲杨亚威张芙蓉孙妮娜
Owner JIANGSU SHENHUA PHARMA
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