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Method for joint production of taxane active ingredients and ethanol by using yew

A technology of taxanes and active ingredients, applied in organic chemistry, biofuels, fermentation, etc., can solve problems such as waste of resources, single enzyme, and underutilization of sugars, and achieve economical cost savings, strong practicability, and easy operation The effect of simple steps and production equipment

Active Publication Date: 2019-05-07
CHONGQING BEISHENG PHARMA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: ① only paclitaxel in the taxane taxanes is purified, ignoring active ingredients such as cephalomannine and 10-DABⅢ; ② enzymatic hydrolysis is single, and only cellulase is used Enzymatic hydrolysis; ③The sugar produced by enzymatic hydrolysis is not fully utilized, resulting in waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The method of utilizing Taxus chinensis to co-produce taxane active ingredients and ethanol of the present embodiment comprises the following steps:

[0032] a. Enzymatic hydrolysis: break the branches and leaves of Taxus chinensis, and add sodium citrate buffer solution with a pH of 4.0 to the broken branches and leaves of Taxus chinensis, with a solid-to-liquid ratio of 1:2, and then add 0.01% of the total amount of yew fibers Sulfase and 0.01% hemicellulase of the total amount of yew, placed at 35 ° C for 10 hours to obtain a hydrolysis mixture containing carbohydrates;

[0033] b. Fermentation: adjust the total sugar concentration of the hydrolyzed mixture in step b to 5 g / L, adjust the pH to 4.5 with citric acid or ammonia, inoculate yeast strains, and ferment for 20 hours at a temperature of 30° C. to obtain a fermentation mixture containing ethanol;

[0034] c. Extraction: adjust the ethanol-containing fermentation mixture to 20% ethanol volume fraction, stir and...

Embodiment 2

[0037] The method of utilizing Taxus chinensis to co-produce taxane active ingredients and ethanol of the present embodiment comprises the following steps:

[0038] a. Enzymatic hydrolysis: break the branches and leaves of Taxus chinensis, and add sodium citrate buffer solution with a pH of 5.5 to the broken branches and leaves of Taxus chinensis, with a solid-to-liquid ratio of 1:10, and then add 0.5% of the total amount of yew fibers Sulfase and 0.5% hemicellulase of the total amount of yew, placed at 45 ° C for 50 hours to obtain a hydrolysis mixture containing carbohydrates;

[0039] b. Fermentation: adjust the total sugar concentration of the hydrolyzed mixture in step b to 50 g / L, adjust the pH to 6.0 with citric acid or ammonia water, inoculate yeast strains, and ferment at a temperature of 35° C. for 50 hours to obtain a fermentation mixture containing ethanol;

[0040] c. Extraction: adjust the ethanol-containing fermentation mixture to 60% ethanol volume fraction, st...

Embodiment 3

[0043] The method of utilizing Taxus chinensis to co-produce taxane active ingredients and ethanol of the present embodiment comprises the following steps:

[0044] a. Enzyme hydrolysis: break the branches and leaves of Taxus chinensis, add pH 5 sodium citrate buffer solution to the broken branches and leaves of Taxus chinensis, the solid-liquid ratio is 1:6, and then add 0.15% fiber of the total amount of Taxus chinensis Sulfase and 0.1% hemicellulase of the total amount of yew, placed at 40°C for reaction h, to obtain a hydrolysis mixture containing carbohydrates;

[0045] b. Fermentation: adjust the total sugar concentration of the hydrolyzed mixture in step b to 30 g / L, adjust the pH to 5.5 with citric acid or ammonia water, inoculate yeast strains, and ferment at a temperature of 32° C. for 35 hours to obtain a fermentation mixture containing ethanol;

[0046] c. Extraction: adjust the ethanol-containing fermentation mixture to 40% ethanol volume fraction, stir and extrac...

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PUM

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Abstract

The invention discloses a method for co-producing taxane effective ingredients and ethyl alcohol by utilizing Chinese yew. The method for co-producing the taxane effective ingredients and ethyl alcohol by utilizing the Chinese yew comprises the following steps: carrying out enzymolysis on cellulose and hemicellulose under a weak acid condition, fermenting for producing ethyl alcohol, and then extracting the taxane effective ingredients by utilizing the produced ethyl alcohol. The cellulose and hemicellulose in the Chinese yew are hydrolyzed, and fermentation is carried out for producing the ethyl alcohol after the cellulose is hydrolyzed by virtue of an enzyme, so that the taxane effective ingredients can be better dissolved into an ethyl alcohol extracting solution, the taxane effective ingredients such as taxol, cephalomannine and 10-DABIII and the ethyl alcohol can be extracted at the same time, extraction ratio of taxol is more than 99%, the extraction ratio of the cephalomannine is more than 99%, the extraction ratio of the 10-DABIII is more than 99%, content of the taxol in extract is 1-5%, content of the cephalomannine is 1-3%, and content of the 10-DABIII is 1-3%.

Description

technical field [0001] The invention relates to the technical field of separating and extracting active ingredients from plants, in particular to a method for co-producing taxane active ingredients and ethanol using yew. Background technique [0002] Taxane compounds are a class of compounds extracted from the bark, roots and leaves of the higher gymnosperm Taxus chinensis, mainly containing paclitaxel, cephalomannine, 10-DABⅢ, 7-xylosyl paclitaxel, etc. There are many Has been shown to have cytotoxic and antitumor activity. The more commonly used methods of extracting and preparing paclitaxel from Taxus plants are generally as follows: first extract with organic solvent, then recycle the organic solvent to obtain extract, degrease the extract and perform column separation, and on this basis Many improved processes have been developed, but these existing processes use a large amount of solvents, which are likely to cause environmental pollution and greatly increase the cost...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12P19/14C07D305/14
CPCC07D305/14C12P7/10C12P19/14Y02E50/10
Inventor 潘先文潘敬坤夏涛张龙
Owner CHONGQING BEISHENG PHARMA TECH CO LTD
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