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Method for detecting oligosaccharide component content in compound salvia miltiorrhiza bge extract

A method of determination, the technology of compound salvia miltiorrhiza, applied in the field of detection of active ingredients of traditional Chinese medicine, can solve problems such as low sensitivity and poor stability

Active Publication Date: 2016-06-15
TIANJIN TASLY MORDEN TCM RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have poor stability and low sensitivity

Method used

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  • Method for detecting oligosaccharide component content in compound salvia miltiorrhiza bge extract
  • Method for detecting oligosaccharide component content in compound salvia miltiorrhiza bge extract
  • Method for detecting oligosaccharide component content in compound salvia miltiorrhiza bge extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Measure according to high performance liquid chromatography (Appendix VID of Chinese Pharmacopoeia 2010 edition).

[0088] Chromatographic conditions and system suitability test

[0089] PrevailTMCarbohydrateES chromatographic column was used, acetonitrile was used as mobile phase A, 0.1% formic acid water was used as mobile phase B, flow rate was 0.8ml / min, column temperature was 30°C; WATERS2420ELSD detector was used for detection, parameters were set to gain 10, air pressure 30psi, drift tube 60°C, Nebheater: 60%. The number of theoretical plates calculated based on the sucrose chromatographic peak should not be less than 4000, and the chromatographic resolution of fructose and glucose should be greater than 2.0.

[0090] time (minutes)

Acetonitrile

0.1% formic acid in water

0

75

25

10

70

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15

60

40

20

75

25

[0091] Preparation of reference substance solution Accurately weigh an a...

Embodiment 2

[0095] Measure according to high performance liquid chromatography (Appendix VID of Chinese Pharmacopoeia 2010 edition).

[0096] Chromatographic conditions and system suitability test

[0097] Adopt PrevailTMCarbohydrateES chromatographic column, use acetonitrile as mobile phase A, 0.1% formic acid water as mobile phase B, flow rate 0.8ml / min, column temperature 30°C; use WATERS2420ELSD detector for detection, parameter settings are gain 10, air pressure 25psi, drift Tube 65°C, Nebheater: 60%. The number of theoretical plates calculated based on the sucrose chromatographic peak should not be less than 4000, and the chromatographic resolution of fructose and glucose should be greater than 2.0.

[0098] time (minutes)

Acetonitrile

0.1% formic acid in water

0

75

25

10

70

30

15

60

40

20

75

25

[0099] Preparation of reference substance solution Accurately weigh an appropriate amount of fructose,...

Embodiment 3

[0103] Measure according to high performance liquid chromatography (Appendix VID of Chinese Pharmacopoeia 2010 edition).

[0104] Chromatographic conditions and system suitability test using PrevailTMCarbohydrateES chromatographic column, with acetonitrile as mobile phase A, 0.2% formic acid water as mobile phase B, flow rate 0.85ml / min, column temperature 30 ° C; detection with WATERS2420ELSD detector, the parameters are set to Gain 10, air pressure 30psi, drift tube 60°C, Nebheater: 60%. The number of theoretical plates calculated based on the sucrose chromatographic peak should not be less than 4000, and the chromatographic resolution of fructose and glucose should be greater than 2.0.

[0105] time (minutes)

Acetonitrile

0.1% formic acid in water

0

75

25

10

70

30

15

60

40

20

75

25

[0106] Preparation of reference substance solution Accurately weigh an appropriate amount of fructose, gluco...

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Abstract

The present invention relates to a method for detecting active ingredients of a traditional Chinese medicine, and in particular relates to a method for detecting oligosaccharide component content in a compound salvia miltiorrhiza bge extract. The method comprises the following steps: (1) preparation of a reference substance solution, to be more specific, weighing fructose, glucose, sucrose, raffinose and Gal-Gal-Glu-Fru tetrasaccharide reference substances, adding water to prepare the mixed reference substance solution; (2) preparation of a to-be-tested substance solution, to be more specific, taking the compound salvia miltiorrhiza bge extract, adding water for dissolving, loading onto a solid phase extraction column, washing with water, collecting a sample loading solution and a washing solution, and adding water to prepare the to-be-tested substance solution; and (3) determination, to be more specific, sucking the mixed reference substance solution and the to-be-tested substance solution, injecting into an evaporative light scattering high performance liquid chromatography with to obtain a chromatogram map, and calculating the oligosaccharide component content in a to-be-tested substance according to the chromatogram map.

Description

Technical field: [0001] The invention relates to a method for detecting effective components of traditional Chinese medicine, in particular to a method for determining the content of oligosaccharide components in compound salvia miltiorrhiza extract. Background technique: [0002] Compound Danshen preparations are cardiovascular drugs prepared from Danshen, Panax notoginseng and borneol. Currently, there are Compound Danshen Capsules, Compound Danshen Tablets and Compound Danshen Dropping Pills on the market. In particular, compound Danshen dripping pills are widely used in the prevention, treatment and first aid of coronary heart disease and angina pectoris. Now it has become one of the leading brands in the domestic cardiovascular market. [0003] Compound Danshen extract is the extract of Danshen and Panax notoginseng. It is the intermediate of compound Danshen preparation. There are many extraction methods. The main active ingredients include protocatechualdehyde, notog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 徐波王苹牛涛陈红
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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