Home smart detection kit
A technology of intelligent detection and kit, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of pathogens that are prone to drug resistance, long-term cure, etc., and achieve the effects of low cost, avoiding the interference of human factors, and easy to carry
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Embodiment 1
[0030] 1. Preparation of monoclonal antibody against Mycoplasma ascites
[0031] Strains: Mycoplasma hominis and Mycoplasma urolyticum were provided by ATCC Company of the United States. The above strains were used as immunogens to produce monoclonal antibodies.
[0032] Cell line: The sp20 mouse myeloma cell line was provided by the Cell Engineering Research Center of Fourth Military Medical University for cell fusion.
[0033] method:
[0034] Preparation and inactivation of the immunogenic Mycoplasma strains: Add 1ml of the liquid culture medium of Mycoplasma hominis and Mycoplasma urolyticum to the freeze-dried powder of standard strains of Mycoplasma hominis and Mycoplasma urealyticum respectively, after dissolving, pipette the Mycoplasma bacteria respectively The solution was added to 10 bottles of Mycoplasma pneumoniae liquid medium, 10 bottles of Mycoplasma hominis liquid medium, and 10 bottles of Mycoplasma urealyticum liquid medium, and 100 μl was added to each bott...
Embodiment 2
[0145] 1. Preparation of monoclonal antibody against Mycoplasma ascites
[0146] Strains: Mycoplasma hominis and Mycoplasma urolyticum were provided by ATCC Company of the United States. The above strains were used as immunogens to produce monoclonal antibodies.
[0147] Cell line: The sp20 mouse myeloma cell line was provided by the Cell Engineering Research Center of Fourth Military Medical University for cell fusion.
[0148] method:
[0149] Preparation and inactivation of the immunogenic Mycoplasma strains: Add 1ml of the liquid culture medium of Mycoplasma hominis and Mycoplasma urolyticum to the freeze-dried powder of standard strains of Mycoplasma hominis and Mycoplasma urealyticum respectively, after dissolving, pipette the Mycoplasma bacteria respectively The solution was added to 10 bottles of Mycoplasma pneumoniae liquid medium, 10 bottles of Mycoplasma hominis liquid medium, and 10 bottles of Mycoplasma urealyticum liquid medium, and 100 μl was added to each bott...
Embodiment 3
[0260] The household intelligent detection kit is composed of two parts: an electronic reading analysis part and a monoclonal antibody detection reagent. The electronic reading analysis part includes a single-chip microcomputer system, a photoelectric detection system, a battery, a switch K, a liquid crystal display, a probe and Test paper, the composite gold standard detection probe, battery, switch K, and single-chip microcomputer system are sealed in the casing, and the liquid crystal display is installed at the window of the casing; the monoclonal antibody detection reagent includes the monoclonal Antibody, Neisseria gonorrhoeae monoclonal antibody, Candida albicans monoclonal antibody, goat anti-mouse IgG antibody, composite gold-labeled detection probe;
[0261] The test paper comprises a gold standard pad, a nitrocellulose membrane, a sample pad, and an absorbent pad;
[0262] The composite gold-labeled detection probe is coated on the gold-labeled pad, and the composit...
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