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Kluyveromyces marxianus yeast application

A yeast and fermentation agent technology, applied in the application field of Kluyveromyces marxianus, can solve problems such as active K.marxianus cells that have not been seen yet, and achieve the goal of improving animal digestion, protecting free radical damage, and optimizing animal immunity Effect

Inactive Publication Date: 2016-06-29
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no research reports or patents on the use of active K.marxianus cells as feed additives in China.

Method used

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  • Kluyveromyces marxianus yeast application
  • Kluyveromyces marxianus yeast application
  • Kluyveromyces marxianus yeast application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, improving the immunity of animal self

[0033] 1) α-1,3-mannan can promote T cell function

[0034] The α-1,3-linked mannosyl on the N-oligosaccharide chain of S.cerevisiae yeast cell wall glycoprotein has high immunogenicity, can increase the release of cytokines, and increase the concentration of interleukin IL-2 to promote T The proliferation and differentiation of cells, and enhance the activity of interferon, thereby improving the non-specific immunity of animals to diseases such as bacteria.

[0035]

[0036]

[0037] Table 1 and Table 2 show the number of key repetitive genes between and within the species of yeast. Table 1 shows the number of repeated genes in each group of 15 strains of yeast between species, and Table 2 shows the number of repeated genes in 6 strains of K. marxianus (K.M.) Number of repeated genes in yeast.

[0038] It can be seen from Table 1 and Table 2 that the copy number of gene ALG3 encoding α-1,3 mannosyltransferas...

Embodiment 2

[0043] Example 2, eliminating harmful D-lactic acid

[0044] Lactic acid is divided into L-lactic acid and D-lactic acid. Humans and animals can consume and utilize L-lactic acid, but cannot metabolize D-lactic acid. Excessive intake can easily cause adverse reactions such as metabolic disorders. In yogurt and lactic acid drinks, mainly L-lactic acid, only a small amount of D-lactic acid.

[0045] Referring to Tables 1 and 2, the gene DLD encoding D-lactate dehydrogenase (capable of converting D-lactate into pyruvate) generally has much more copies in the genus Kluyveromyces than in other yeast species, which means that K. marxianus There is a greater capacity for D-lactate depletion, thereby protecting animals from D-lactosis.

Embodiment 3

[0046] Example 3, Reducing Lactose Intolerance

[0047] It has been reported that some piglets have lactose intolerance, that is, when there is a lack of lactase, the ingested lactose stays in the intestinal tract and is fermented and utilized by harmful intestinal bacteria to produce acetic acid, propionic acid, butyric acid, etc., and produce a large amount of Gas including CH 4 、H 2 , CO 2 , leading to abdominal distension and diarrhea.

[0048] refer to Figure 2C Some genes involved in lactose utilization in K.marxianusCGMCCNo.10621, such as GAL4 and HNT1, are deleted in the existing strains K.marxianusNBRC1777 and DMKU3-1042, but exist in CGMCCNo.10621, that is, it means that K.marxianusCGMCCNo.10621 starts with After being added to the feed in the form of active dry powder, it can metabolize lactose in the intestinal tract of animals and improve the intestinal health of animals, especially piglets.

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Abstract

The present invention provides an application of kluyveromyces marxianus yeast, especially the applications in feed additives and feed fermentation, and relates to a feed and a feed additive. The kluyveromyces marxianus yeast is preferably used as an animal feed additive and / or an animal feed fermentation agent, and can improve the immune system of animals, and / or improve the digestion ability of animals, and / or protect animals from free radical damage, and / or produce more nutrients. More preferably, compared to conventional yeasts, the kluyveromyces marxianus yeast can better improve animal immunity, and / or improve animal digestion, and / or protect animals from free radical damage, and / or produce more nutrients.

Description

technical field [0001] The present invention relates to an application of Kluyveromyces maximus, in particular to an animal feed, an animal feed fermentation method, a feed additive and the application of Kluyveromyces maximus CGMCC No. 10621 in feed fermentation or feed additive. Background technique [0002] Kluyveromyces marxianus (Kluyveromyces marxianus, K.marxianus) is ubiquitous in nature in kumiss (KhrisanfovaLP, AntimicrobialpropertiesofKoumissfromcowandmaremilk, Moloch.Prom., 1969,30 (10): 16), sour milk (FleetGH, Spoilageyeasts, CritRevBiotechnol., 1992,12:1-44; TudorA et al., Foodspoilageyeasts, InRoseAH, HarrisonJS (eds)Theyeasts, vol5, 2ndedn.Academic Press, London, 1993:435-515.), milk (WendelB.Silveira et al., GenomicSequenceoftheYeastKluyveromycesmarxianus(UCT77 3), a HighlyLactose-Fermenting Yeast Isolated from theBrazilianDairyIndustry, GenomeAnnouncements, 2014, 2(6):e01136-14), fruit (LodderJ et al, Theyeasts: ataxonomics study, NHPC, Amsterdam, 1952), s...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K50/00
CPCA23V2002/00A23V2250/76
Inventor 吕红莫文娟周峻岗余垚
Owner FUDAN UNIV
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