Micro-fluidic chip and nucleic acid extraction and purification method therewith

A technology of a microfluidic chip and a purification method, which is applied in the field of microfluidic chip and nucleic acid extraction and purification using the microfluidic chip, can solve the problems of environmental pollution, time-consuming and cumbersome, and inflexible operation, and achieves the realization of spatial and temporal resolution information. , Avoid manual operation, optimize the effect of purification process

Active Publication Date: 2016-07-06
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction methods commonly used in laboratories have many disadvantages: such as phenol/chloroform extraction method and spin column method, which require multiple centrifuges, have relatively high requirements for external equipment, and are easy to cause sample loss; and the use of organic solvents will also cause environmental pollution. Pollution, endangering

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Micro-fluidic chip and nucleic acid extraction and purification method therewith
  • Micro-fluidic chip and nucleic acid extraction and purification method therewith
  • Micro-fluidic chip and nucleic acid extraction and purification method therewith

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Extraction and purification of lambda DNA

[0065] separately to figure 1 Add 6ng / μL λDNA solution into the sample pool 4 of the chip shown, and add superparamagnetic silicon beads, loading buffer, washing solution and eluent into inlet 1. Under the control of the pneumatic microvalve, the λDNA and superparamagnetic silicon beads The sample buffer, cleaning solution and eluent enter the mixing unit under the condition of vacuum driving force, and the pneumatic microvalve is also controlled to realize the sufficient mixing and purification of the nucleic acid extraction buffer and λDNA, and the sample buffer, cleaning solution and washing solution are collected respectively. dehydration.

[0066] Specific steps include:

[0067] 1. Fully mix the sample solution containing λDNA with superparamagnetic nanoparticles. Under the acidic condition of pH 6.1, the nucleic acid and superparamagnetic nanoparticles combine to form nucleic acid-magnetic nanoparticle complexes and a...

Embodiment 2

[0070] Extraction and purification of real sample DNA

[0071] separately to figure 1 Cell lysates (eukaryotic cells or prokaryotic cells) are respectively added to the sample pool 4 of the chip shown, and superparamagnetic silica beads, sample loading buffer, washing solution and eluent are added to the inlet 1, and controlled by a pneumatic microvalve, The real sample, superparamagnetic silica beads, loading buffer, cleaning solution and eluent enter the mixing unit under the condition of vacuum driving force, and the pneumatic microvalve is also controlled to realize the full mixing of nucleic acid extraction buffer and lysed cell buffer, Purify, and finally collect the eluate containing the purified DNA sample. The specific steps include: 1. Fully mixing the lysed cell solution with the superparamagnetic nanoparticles, and under the acidic condition of pH 6.1, the nucleic acid and the superparamagnetic nanoparticles combine to form a nucleic acid-magnetic nanoparticle com...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a micro-fluidic chip and a nucleic acid extraction and purification method therewith. The micro-fluidic chip has a four-layer membrane structure including: a liquid path layer, a gas path control layer, a magnet control layer and a glass substrate layer. The gas path control layer is arranged below the liquid path layer. The substrate layer is arranged below the gas path control layer. The magnet control layer is arranged below the glass substrate layer. The method, with the micro-fluidic chip, includes the steps of: a) introducing a target sample; b) introducing superpara-magnetic silicon beads for nucleic acid purification; and c) extracting and purifying nucleic acid from the mixed sample. In the invention, introduction of the sample, cleaning of non-nucleic acid substances and collection and purification of the nucleic acid are integrated on one micro-fluidic chip, so that the nucleic acid extraction and purification process is optimized and extraction and purification efficiency is greatly improved. The method simplifies manual operations and achieves temporal-spatial resolution information which is difficult in conventional methods, and has excellent nucleic acid extraction and purification effects.

Description

technical field [0001] The invention relates to the field of nucleic acid extraction and purification, in particular to a microfluidic chip and a nucleic acid extraction and purification method using the microfluidic chip. Background technique [0002] The separation and purification technology of biomacromolecular ribonucleic acid is the basis for all aspects of molecular biology research and a key technology in life science research and application. The efficiency and quality of nucleic acid sample preparation will directly affect the results of subsequent experiments. At present, the extraction methods commonly used in laboratories have many disadvantages: such as phenol / chloroform extraction method and spin column method, which require multiple centrifuges, have relatively high requirements for external equipment, and are easy to cause sample loss; and the use of organic solvents will also cause environmental pollution. Contamination endangers the health of the operator...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12M1/00C12N15/10
Inventor 秦建华苏文涛姜雷李艳峰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap