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Liquid culture medium used for cultivating pleurotus geesteranus mycelia and cultivation method thereof

A technology for liquid culture medium and rare mushroom is applied in the field of liquid culture medium and liquid submerged fermentation culture, and cultivation of rare mushroom can solve the problems of high cost of liquid medium, poor culture effect, unsuitability for large-scale industrial production, etc. Low cost and good cultivation effect

Inactive Publication Date: 2016-07-06
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the advantages of liquid submerged fermentation culture are great, the current liquid medium is not good for the cultivation of Pleurotus osmanthus mycelium in liquid submerged fermentation, and the cost of liquid medium is relatively high, so it is not suitable for large-scale industrial production
At the same time, the technology of submerged liquid fermentation to cultivate Pleurotus erodes mycelia is still in the small-scale shake flask stage, and rarely carried out in large-scale fermentation tanks.
For large-scale liquid submerged fermentation culture, the control of various parameters in the cultivation process plays a very important role in the quality of the whole fermentation, but there is no technology applicable to large-scale liquid submerged fermentation cultivation of Pleurotus edodes

Method used

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  • Liquid culture medium used for cultivating pleurotus geesteranus mycelia and cultivation method thereof
  • Liquid culture medium used for cultivating pleurotus geesteranus mycelia and cultivation method thereof
  • Liquid culture medium used for cultivating pleurotus geesteranus mycelia and cultivation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0062] The present embodiment provides a method for cultivating Pleurotus erodes mycelium by liquid submerged fermentation, which comprises:

[0063] Weigh 1.5kg of peeled potatoes, add appropriate amount of water to boil for 18min, filter with 4 layers of gauze to obtain the filtrate, add 0.15kg of agar powder and 0.15kg of sucrose to the filtrate, add water to a total volume of 10L, mix, melt, and pack , sterilized at 120°C for 18 minutes to obtain the initial slant medium; put the initial slant medium into a constant temperature incubator and cultivate it for 2 days, observe whether there are any miscellaneous bacteria such as mold, if not, it is the slant medium that can be inoculated.

[0064] Inoculate the mother species of Chrysanthemum pleurotus on the slant medium, and the quality of the mother species of Pleurotus osmanthus is 3% of the mass of the slant medium. Place the inoculated slant medium in a constant temperature incubator at 26-30° C. for 3 days, and the sla...

Embodiment 2

[0070] The present embodiment provides a method for cultivating Pleurotus erodes mycelium by liquid submerged fermentation, which comprises:

[0071] Weigh 2.5kg of peeled potatoes, add appropriate amount of water to boil for 22min, filter with 5 layers of gauze to obtain the filtrate, add 0.25kg of agar powder and 0.25kg of sucrose to the filtrate, add water to a total volume of 10L, mix, melt, and pack , sterilized at 122°C for 22 minutes to obtain the initial slant medium; put the initial slant medium into a constant temperature incubator and cultivate it for 3 days, observe whether there are molds and other miscellaneous bacteria, if not, it is the slant medium that can be inoculated.

[0072] Inoculate the mother species of Chrysanthemum pleurotus on the slant medium, and the quality of the mother species of Pleurotus osmanthus is 5% of the mass of the slant medium. Place the inoculated slant medium in a constant temperature incubator at 30°C for 5 days, and when the slan...

Embodiment 3

[0078] The present embodiment provides a method for cultivating Pleurotus erodes mycelium by liquid submerged fermentation, which includes:

[0079] Weigh 3kg of peeled potatoes, add an appropriate amount of water to boil for 20min, filter with 4 layers of gauze to obtain the filtrate, add 0.2kg of agar powder, 0.2kg of sucrose to the filtrate, add water to a total volume of 10L, mix, melt, and pack. Sterilize at 121°C for 20 minutes to obtain the initial slant medium; put the initial slant medium into a constant temperature incubator and incubate for 3 days, observe whether there are any miscellaneous bacteria such as mold, if not, it is the slant medium that can be inoculated.

[0080] Inoculate the mother species of Pleurotus chinensis on the slant culture medium, and the quality of the mother species of Pleurotus chinensis is 4% of the mass of the slant medium. Place the inoculated slant medium in a constant temperature incubator at 28°C for 4 days, and when the slant medi...

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Abstract

The invention provides a liquid culture medium used for cultivating pleurotus geesteranus mycelia and relates to the field of strain culture. The liquid culture medium comprises the following components by weight percent: 3-5% of a carbon source, 3.5-6.5% of a nitrogen source, 0.08-0.12% of inorganic salt, 0.0035-0.0065% of vitamin and water; the liquid culture medium adopts an appropriate carbon source, a nitrogen source, inorganic salt and vitamin and ratio, all the raw materials of the liquid culture medium are synergistic, the culture effect on the pleurotus geesteranus mycelia is good, and the cost is low, so that the liquid culture method is applicable to large-scale submerged liquid fermentation culture of the pleurotus geesteranus mycelia. The invention also provides a method for cultivating the pleurotus geesteranus mycelia through submerged liquid fermentation. The method utilizes the liquid culture medium for carrying out activation amplification fermentation culture on pleurotus geesteranus original strains for multiple times and is applicable to the large-scale submerged liquid fermentation culture of the pleurotus geesteranus mycelia.

Description

technical field [0001] The invention relates to the field of strain culture, and in particular to a liquid culture medium and a liquid submerged fermentation culture method for cultivating pear mushrooms. Background technique [0002] As an important biological resource, edible fungi are not only delicious, low in fat and low in energy, but also rich in nutrients such as dietary fiber, vitamins and protein, and are developing into the third type of food after animal food and plant food. , that is, fungal food. In addition, edible fungi can also improve the immune function of the human body, prevent and treat various chronic diseases, and have significant effects in anti-aging and other aspects. Among many edible fungi, xiuzhen mushroom is widely eaten because of its delicious taste, high nutritional value, and simple planting conditions. Xiuzhen mushroom belongs to the fungus door, Agaricaceae, Basidiomycetes, Pleurotaceae, Pleurotus genus, also known as small oyster mushr...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 余晓红李锋李凤伟薛锋彭英云张红徐汉亿郑清
Owner YANCHENG INST OF TECH
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