Liquid culture medium used for cultivating pleurotus geesteranus mycelia and cultivation method thereof

A technology for liquid culture medium and rare mushroom is applied in the field of liquid culture medium and liquid submerged fermentation culture, and cultivation of rare mushroom can solve the problems of high cost of liquid medium, poor culture effect, unsuitability for large-scale industrial production, etc. Low cost and good cultivation effect

Inactive Publication Date: 2016-07-06
YANCHENG INST OF TECH
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the advantages of liquid submerged fermentation culture are great, the current liquid medium is not good for the cultivation of Pleurotus osmanthus mycelium in liquid submerged fermentation, and the cost of liquid medium is relatively high, so it is not suitable for large-scale industrial production
At the same time, the technology of submerged liquid fermentation to cultivate Pleurotus erodes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Liquid culture medium used for cultivating pleurotus geesteranus mycelia and cultivation method thereof
  • Liquid culture medium used for cultivating pleurotus geesteranus mycelia and cultivation method thereof
  • Liquid culture medium used for cultivating pleurotus geesteranus mycelia and cultivation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0061] Example 1

[0062] The present embodiment provides a method for cultivating Pleurotus erodes mycelium by liquid submerged fermentation, which comprises:

[0063] Weigh 1.5kg of peeled potatoes, add appropriate amount of water to boil for 18min, filter with 4 layers of gauze to obtain the filtrate, add 0.15kg of agar powder and 0.15kg of sucrose to the filtrate, add water to a total volume of 10L, mix, melt, and pack , sterilized at 120°C for 18 minutes to obtain the initial slant medium; put the initial slant medium into a constant temperature incubator and cultivate it for 2 days, observe whether there are any miscellaneous bacteria such as mold, if not, it is the slant medium that can be inoculated.

[0064] Inoculate the mother species of Chrysanthemum pleurotus on the slant medium, and the quality of the mother species of Pleurotus osmanthus is 3% of the mass of the slant medium. Place the inoculated slant medium in a constant temperature incubator at 26-30° C. for...

Example Embodiment

[0069] Example 2

[0070] The present embodiment provides a method for cultivating Pleurotus erodes mycelium by liquid submerged fermentation, which comprises:

[0071] Weigh 2.5kg of peeled potatoes, add appropriate amount of water to boil for 22min, filter with 5 layers of gauze to obtain the filtrate, add 0.25kg of agar powder and 0.25kg of sucrose to the filtrate, add water to a total volume of 10L, mix, melt, and pack , sterilized at 122°C for 22 minutes to obtain the initial slant medium; put the initial slant medium into a constant temperature incubator and cultivate it for 3 days, observe whether there are molds and other miscellaneous bacteria, if not, it is the slant medium that can be inoculated.

[0072] Inoculate the mother species of Chrysanthemum pleurotus on the slant medium, and the quality of the mother species of Pleurotus osmanthus is 5% of the mass of the slant medium. Place the inoculated slant medium in a constant temperature incubator at 30°C for 5 day...

Example Embodiment

[0077] Example 3

[0078] The present embodiment provides a method for cultivating Pleurotus erodes mycelium by liquid submerged fermentation, which includes:

[0079] Weigh 3kg of peeled potatoes, add an appropriate amount of water to boil for 20min, filter with 4 layers of gauze to obtain the filtrate, add 0.2kg of agar powder, 0.2kg of sucrose to the filtrate, add water to a total volume of 10L, mix, melt, and pack. Sterilize at 121°C for 20 minutes to obtain the initial slant medium; put the initial slant medium into a constant temperature incubator and incubate for 3 days, observe whether there are any miscellaneous bacteria such as mold, if not, it is the slant medium that can be inoculated.

[0080] Inoculate the mother species of Pleurotus chinensis on the slant culture medium, and the quality of the mother species of Pleurotus chinensis is 4% of the mass of the slant medium. Place the inoculated slant medium in a constant temperature incubator at 28°C for 4 days, and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a liquid culture medium used for cultivating pleurotus geesteranus mycelia and relates to the field of strain culture. The liquid culture medium comprises the following components by weight percent: 3-5% of a carbon source, 3.5-6.5% of a nitrogen source, 0.08-0.12% of inorganic salt, 0.0035-0.0065% of vitamin and water; the liquid culture medium adopts an appropriate carbon source, a nitrogen source, inorganic salt and vitamin and ratio, all the raw materials of the liquid culture medium are synergistic, the culture effect on the pleurotus geesteranus mycelia is good, and the cost is low, so that the liquid culture method is applicable to large-scale submerged liquid fermentation culture of the pleurotus geesteranus mycelia. The invention also provides a method for cultivating the pleurotus geesteranus mycelia through submerged liquid fermentation. The method utilizes the liquid culture medium for carrying out activation amplification fermentation culture on pleurotus geesteranus original strains for multiple times and is applicable to the large-scale submerged liquid fermentation culture of the pleurotus geesteranus mycelia.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner YANCHENG INST OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap