Culture medium and culture method for efficiently producing poria cocos mycelia and cellulase

Abstract

The invention discloses a culture medium and a culture method for efficiently producing poria cocos mycelia and cellulase. The method comprises the following steps briefly: adding crushed substances rich in cellulose into a prepared liquid inorganic salt basic culture medium, performing high-temperature sterilization on the culture medium, cooling the culture medium to room temperature, adding a small amount of microelements and growth factors into the culture medium, inoculating activated poria cocos mycelia into a triangular flask containing the culture medium of which the volume is not more than 10% of that of a triangular flask, culturing the poria cocos mycelia at room temperature until the poria cocos mycelia are full of the bottom of the triangular flask, transferring the mycelia to another triangular flask containing the culture medium according to a ratio of about 1:50, culturing the mycelia at room temperature for 2 weeks, filtering to obtain poria cocos mycelia and a culture liquid, and assaying the culture liquid by using 3,5-dinitrosalicylic acid to obtain that the cellulase in bacterial liquid reaches 120 IU / ml or above. According to the method disclosed by the invention, a simple inorganic salt and a fiber substance are used as the culture medium, the poria cocos mycelia and a culture liquid of high-activity cellulase are obtained at room temperature without a swing bed, so that the method not only is beneficial efficient production of the poria cocos mycelia and the utilization of wastes rich in cellulose, but also can save energy and resources.

Description

technical field [0001] The present invention relates to a liquid culture medium for efficiently producing Poria cocos mycelia and cellulase and a culture method thereof, in particular to a culture medium with simple inorganic salts and cellulose-rich waste as the main components of the culture medium without shaking The bed and constant temperature culture can rapidly cultivate Poria cocos mycelium and obtain a culture solution containing high-concentration cellulase. Using this method, it is not only beneficial to the utilization and development of cellulose-rich waste, but also saves energy and resources. Background technique [0002] Poria, commonly known as Yunling, Songling, Fuling, etc., its protozoa is the dry sclerotia of Polyporaceae fungus Poria, and it is mostly parasitic on the roots of Pinus massoniana or Pinus massoniana. Poria cocos is the most widely used fungus in traditional Chinese medicine prescriptions. Pharmacological studies have also confirmed that it...

AI Technical Summary

Problems solved by technology

From planting Poria cocos to pre-processing, it requires a lot of resources and energy consumption

Poria cocos produced in different origins have great differences in quality, which also affects the quality control of poria cocos products

Therefore, at present, many researchers use liquid fermentation to cultivate Poria cocos, but the composition of the liquid medium contains a large amount of yeast extract, peptone and glucose. method, and need to control temperature and oxygen supply, high power consumption, so it is not economical and environmentally friendly

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