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Eucommia ulmoides chitinase encoding gene (EuCHIT1) and application thereof

A chitinase and coding technology, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems that the study of Eucommia chitinase gene has not been reported yet.

Inactive Publication Date: 2016-07-06
GUIZHOU UNIV
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Problems solved by technology

At present, the chitinase gene sequences of many plants have been cloned, such as poplar, broad bean, wheat, rice, sugar beet, tobacco, potato, cotton, etc., but the study of Eucommia chitinase gene has not been reported yet.

Method used

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  • Eucommia ulmoides chitinase encoding gene (EuCHIT1) and application thereof
  • Eucommia ulmoides chitinase encoding gene (EuCHIT1) and application thereof
  • Eucommia ulmoides chitinase encoding gene (EuCHIT1) and application thereof

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Embodiment Construction

[0027] In order to explain the purpose, technical solutions and advantages of the present invention more clearly, the present invention will be further described in detail in conjunction with the accompanying drawings. The specific implementation methods described here are only used to explain the present invention.

[0028] In this experiment, Eucommia ulmoides planted more than 10 years old in the experimental demonstration base of Guizhou Agricultural Bioengineering Key Laboratory was used as the material, and the young shoots, leaves, young branches, bark and young fruits of the male and female plants were collected to extract total RNA and reverse cDNA.

[0029] Total RNA was extracted using Omega's The difficult sample of PlantRNAKit is described. The extracted sample is washed twice with absolute ethanol to remove excess salt. The final extracted product is dissolved in an appropriate amount of 40ulRnase-free water and stored at -80°C. The preparation method of 5'- an...

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Abstract

The invention provides a eucommia ulmoides chitinase encoding gene (EuCHIT1) and application thereof and belongs to the technical field of biological engineering. According to a eucommia ulmoides transcriptome database, a chitinase gene partial segment is annotated, a chitinase gene shown in SEQ ID NO:1 is cloned from eucommia ulmoides by virtue of an SMART RACE technology, and a gene sequence and a translation amino acid of the chitinase gene are as shown in SEQIDNO:2. On the basis of carrying out bioinformatics analysis on the chitinase gene, genetic transformation is carried out on tobacco, in-vitro antibacterial experiments carried out by extracting tobacco total protein show that the chitinase gene has an obvious inhibitory effect on fusaria and botrytis cinerea. According to the invention, for further utilizing a bioengineering technology, an exogenous chitinase gene is transferred into various plants, so that the antifungal capability of the plants is improved, and a foundation is laid for cultivating disease-resistant varieties.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and mainly relates to eucommia antifungal chitinase (EuCHIT1), its coding gene and its application. Background technique [0002] Eucommia ulmoides Oliver (Eucommiaulmoides Oliver) is a dioecious plant of the genus Eucommia ulmoides. The history of its use in my country can be traced back to the "Shen Nong's Herbal Classic" in the second century AD. It has two-way blood pressure regulation, antibacterial and antibacterial effects, and has important medicinal value. . In view of its ancient origin, it has important research value in science. At present, the research on Eucommia mainly focuses on blood pressure regulating substances, cloning of genes related to Eucommia glue synthesis pathway and chemical separation of antibacterial substances. Early studies have shown that the total protein extracted from Eucommia can significantly inhibit the growth of a variety of plant pathoge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N15/56C12N15/84A01H5/00A01P3/00
CPCC12N9/2442C12N15/8282C12Y302/01014
Inventor 赵德刚董璇郭林霞李岩
Owner GUIZHOU UNIV
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