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Preparation method for vincristine molecular imprinting sensor

A technology of molecular imprinting and vincristine, applied in instruments, scientific instruments, analytical materials, etc., can solve problems such as poor regeneration and reversibility, high detection limit, slow electron transfer speed and response, and achieve high affinity and selectivity Sexuality, the effect of response improvement

Inactive Publication Date: 2016-07-06
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the thickness of the imprinted film prepared by the traditional imprinting method is difficult to control, and the high cross-linking degree makes the electron transfer speed and response slow, the detection limit is high, and the regeneration and reversibility are poor, which affects the application of molecular imprinting technology in electrochemical sensors.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Nano-gold modified glassy carbon electrode: the glassy carbon electrode is sequentially coated with 0.3 μm, 0.05 μm Al 2 o 3 The powder is surface polished, then ultrasonically cleaned with high-purity water, dried with nitrogen, and the glassy carbon electrode is placed in an ethanol solution containing 20% ​​by mass of 3-mercaptopropyltriethoxysilane, and the temperature rises to 50± 2°C, soak for 7 hours, take it out and wash it fully with absolute ethanol, blow it dry with nitrogen, soak the glassy carbon electrode in 0.15mol / L gold chlorate solution for 5 hours, and then put it in with a concentration of 20% by mass Soak in the hydrazine hydrate solution for 10 minutes, take it out and wash it with absolute ethanol, and dry it with nitrogen to get the nano-gold modified glassy carbon electrode;

[0020] (2) Preparation of imprinted sol: In the reactor, add ethanol: 9.0mL, graphene oxide: 0.6g, methyl acrylate: 1.3g, itaconic acid: 0.8g, ammonium persulfate: 0....

Embodiment 2

[0023] (1) Nano-gold modified glassy carbon electrode: the glassy carbon electrode is sequentially coated with 0.3 μm, 0.05 μm Al 2 o 3 The powder is surface polished, then ultrasonically cleaned with high-purity water, dried with nitrogen, and the glassy carbon electrode is placed in an ethanol solution containing 18% by mass of 3-mercaptopropyltriethoxysilane, and the temperature rises to 50± 2°C, soak for 8 hours, take it out and wash it fully with absolute ethanol, blow it dry with nitrogen, soak the glassy carbon electrode in 0.1mol / L gold chlorate solution for 6 hours, and then put it in with a mass percentage concentration of 20% Soak in the hydrazine hydrate solution for 10 minutes, take it out and wash it with absolute ethanol, and dry it with nitrogen to get the nano-gold modified glassy carbon electrode;

[0024] (2) Preparation of imprinted sol: In the reactor, add ethanol: 7.8mL, graphene oxide: 0.8g, methyl acrylate: 1.1g, itaconic acid: 1.0g, ammonium persulfat...

Embodiment 3

[0027] (1) Nano-gold modified glassy carbon electrode: the glassy carbon electrode is sequentially coated with 0.3 μm, 0.05 μm Al2 o 3 The powder is surface polished, then ultrasonically cleaned with high-purity water, dried with nitrogen, and the glassy carbon electrode is placed in an ethanol solution containing 23% by mass of 3-mercaptopropyltriethoxysilane, and the temperature rises to 50± 2°C, soak for 6 hours, take it out and wash it fully with absolute ethanol, blow it dry with nitrogen, soak the glassy carbon electrode in 0.2mol / L gold chlorate solution for 4 hours, and then put it in with a concentration of 20% by mass Soak in the hydrazine hydrate solution for 10 minutes, take it out and wash it with absolute ethanol, and dry it with nitrogen to get the nano-gold modified glassy carbon electrode;

[0028] (2) Preparation of imprinted sol: In the reactor, add ethanol: 9.0mL, graphene oxide: 0.5g, methyl acrylate: 1.0g, itaconic acid: 0.5g, ammonium persulfate: 0.5g, s...

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Abstract

The invention discloses a preparation method for a vincristine molecular imprinting sensor.The method is characterized in that firstly, a glassy carbon electrode is modified with 3-mercaptopropyltriethoxysilane and gold chlorate and prepared into a nanogold modified glassy carbon electrode; secondly, a sol-gel imprinting technology, a layer-by-layer self-assembly method and a drip coating method are combined, and the vincristine imprinting electrochemical sensor with specific selectivity is successfully developed on the surface of the modified glassy carbon electrode.The response of the prepared vincristine molecular imprinting sensor is greatly improved.The imprinting sensor shows high affinity and selectivity on vincristine.The vincristine molecular imprinting sensor is connected with an electrochemical workstation to form a dedicated template molecular recognition sensor.The prepared sensor is low in cost, high in sensitivity, good in specificity, quick in detection and capable of being used repeatedly.

Description

technical field [0001] The present invention relates to a method for preparing a molecularly imprinted sensor and the technical field of rapid detection application, in particular to a method for preparing a molecularly imprinted sensor for detection of vincristine, specifically based on molecularly imprinted specific recognition, for detecting drugs, Vincristine in biological samples. Background technique [0002] Vincristine can fight cancer, and its curative effect is about 10 times higher than that of vinblastine. It can be used to treat acute lymphocytic leukemia with better curative effect. There are also curative effects. Alkaloids contained in Vincamajor together with vinblastine, panloxin and panloxetine. It has the effect of stopping cell division (mitosis) in metaphase, which is similar to colchicine, but its effect is stronger than that of colchicine. Like colchicine, it can bind to tubulin to inhibit its biological activity, but the binding site is different....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/308
Inventor 李慧芝翟殿棠许崇娟
Owner UNIV OF JINAN