Continuous release compositions made from hyaluronic acid, and therapeutic applications of same

A technology of hyaluronic acid and hyaluronic acid salt, applied in the field of preventing skin aging and/or repairing dermal tissue, which can solve problems such as uncontrollable size

Active Publication Date: 2016-07-06
伊伯萨制药有限公司
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of preparing this emulsion also showed reproducibility problems due to the uncontrollable size of the droplets formed
Finall

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Continuous release compositions made from hyaluronic acid, and therapeutic applications of same
  • Continuous release compositions made from hyaluronic acid, and therapeutic applications of same
  • Continuous release compositions made from hyaluronic acid, and therapeutic applications of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Preparation of polymer particles according to the invention:

[0075] Step 1: "Primary" Emulsification

[0076] An aqueous hyaluronic acid solution was prepared by dissolving 50 mg of hyaluronic acid in 5 ml of a 4 wt % polyvinyl alcohol (PVA) solution. Separately, an organic solution of the polymer was prepared by dissolving 900 mg of PLGA-PEG-PLGA triblock polymer in 12 ml of dichloromethane / acetone (3 / 1 v / v) mixture. use The two solutions were emulsified at room temperature for two minutes while stirring with an IKAT25 basic stirrer at 16,000 rpm while magnetically stirring at a rate of 500 rpm with a magnetic bar. A second stirring cycle was performed in the same manner in an ice bath.

[0077] Step 2: "Secondary" Emulsification

[0078] The stable emulsion obtained from step 1 was introduced into a glass syringe and injected into a formulation reactor containing 450 ml of 4 wt% polyvinyl alcohol (PVA) under magnetic stirring at a rate of 750 rpm.

[0079] Ste...

Embodiment 2

[0114] Preparation of polymer particles according to the invention:

[0115] The polymer particles according to the invention were prepared in a similar manner to Example 1. The only change relative to the method of Example 1 is that a Silverson stirrer at a rate of 2800 rpm was used instead of the magnetic stirring at 750 rpm in Example 1 in the "secondary" emulsification preparation in the second step.

[0116] Analysis of the inventive polymer particles according to Example 2:

[0117] Ten batches of polymer particles according to the invention were analyzed. The results obtained are as follows:

[0118] - according to use 3 Coulter counter (BeckmanCoulter) particle size analysis, the size of the particles obtained from polydispersity: 34.98 μm ± 7.02 μm (before irradiation) and 34.12 μm ± 7.23 μm (after irradiation),

[0119] - content of hyaluronic acid: 16 μg of hyaluronic acid loaded per mg of freeze-dried polymer particles, and

[0120] - The binding yield of hya...

Embodiment 3

[0128] Preparation of polymer particles according to the invention:

[0129] A method similar to that of Example 2 was used for the preparation of the polymer particles according to the invention. The only change relative to the method of Example 2 is the addition of a pharmaceutically acceptable excipient which is also anti-inflammatory (radical scavenger), i.e. 1 ml of sorbitol in the form of an ultrapure aqueous solution of 10 wt% sorbitol Substitute the ultrapure water used in Example 2.

[0130] Analysis of the inventive polymer particles according to Example 3:

[0131] Six batches of polymer particles according to the invention were analyzed. The results obtained are as follows:

[0132] - according to use 3 Particle size analysis by Coulter counter (BeckmanCoulter), the size of the particles obtained from polydispersity: 31.15 μm ± 7.80 μm, and

[0133] - The content of hyaluronic acid is: 13.94 μg of hyaluronic acid is loaded per mg of freeze-dried polymer parti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Sizeaaaaaaaaaa
Molecular weightaaaaaaaaaa
Login to view more

Abstract

The present invention concerns polymer particles made from poly(lactic-co-glycolic acid) (PLGA) polymer, poly(lactic-co-glycolic acid)-polyethylene glycol-poly(lactic-co-glycolic acid) (PLGA-PEG-PLGA) copolymer, or the mixture of same, combined with hyaluronic acid molecules or hyaluronic acid salts, and the method for preparing same. The present invention also concerns injectable pharmaceutical or cosmetic compositions comprising such polymer particles, the method for preparing such compositions, and the use thereof for medical purposes, in particular for the prevention and/or treatment of musculoskeletal diseases, diseases and traumatic conditions of the skin, oral disorders, vaginal mucosa dryness and urinary infections or cystitis, dryness of the eye membrane and eye infections, obesity, and the use of same to combat ageing of the skin and/or for repairing the dermal tissue (mesotherapy). More particularly, the compositions of the invention can be intended to supplement joint fluids, and in particular synovial fluid, restoring the physiological and rheological conditions of pathological joints, for example arthritic joints, or indeed for treating and repairing the epidermis by remodelling, hydrating and protecting the skin.

Description

technical field [0001] The present invention relates to polymer particles combined with hyaluronic acid molecules or hyaluronate, based on poly(lactic-co-glycolic acid) (PLGA) polymers, based on poly(lactic acid -co-glycolic acid)-polyethylene glycol-poly(lactic-co-glycolic acid) (PLGA-PEG-PLGA) copolymers, or mixtures based on them. Another subject of the present invention are injectable cosmetic or pharmaceutical compositions containing such polymer particles, processes for the preparation of such compositions and their use as medicaments (in particular for the prophylaxis of and / or treatment of: musculoskeletal disorders, traumatic conditions of the skin and skin diseases, oral symptoms, dry vaginal mucosa and urinary tract infections or cystitis, dry eye membranes and eye infections, and obesity), and their role in preventing skin aging and / or Or use in repairing dermal tissue (mesotherapy). More specifically, the compositions of the invention may be aimed at replenishin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K9/16A61K31/728A61K9/10
CPCA61K9/0019A61K9/0024A61K9/1641A61K8/86A61K2800/91A61K31/728A61K8/735A61Q19/08A61P17/00A61P19/00A61K9/10A61K9/14A61K47/10
Inventor 扬尼斯·吉尔曼多米尼克·瓦谢托马斯·佩里厄莫德·贡内特让-诺埃尔·古兹
Owner 伊伯萨制药有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap