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Bacillus licheniformis and microbial agent and their application in fermentation bed culture

A technology of Bacillus licheniformis and microbial agents, applied in the field of agricultural biology, can solve the problems of low efficiency in decomposing feces, and achieve the effects of prolonging working life, reducing odor, and efficiently degrading

Active Publication Date: 2016-07-13
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defects such as low efficiency of decomposing feces existing in the existing fermentation bed strains, the present invention provides a Bacillus licheniformis with higher efficiency of decomposing feces

Method used

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  • Bacillus licheniformis and microbial agent and their application in fermentation bed culture
  • Bacillus licheniformis and microbial agent and their application in fermentation bed culture
  • Bacillus licheniformis and microbial agent and their application in fermentation bed culture

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preparation example Construction

[0020] Wherein, the preparation method of the microbial agent may include: inoculating Bacillus licheniformis with a preservation number of CGMCC No. 11235 in the medium for cultivation. Preferably, the viable count of Bacillus licheniformis with a preservation number of CGMCC No. 11235 can be 10 per gram of the microbial agent obtained by culturing. 7 -10 11 cfu, more preferably 10 9 -10 10 cfu. During the cultivation process, the concentration of live bacteria can be obtained by conventional methods, such as hemocytometer or OD value observation.

[0021] The cultured bacterial solution can be directly used as a microbial bacterial agent. Preferably, the bacterial solution is further processed into a microbial bacterial agent in a dosage form that is more convenient for storage through steps including aseptic filtration and freeze-drying. Wherein, the culture conditions of the strains are not particularly limited, and may be the common conditions in the culture process o...

Embodiment 1

[0033] This example is used to illustrate the cultivation of Bacillus licheniformis of the present invention and the preparation of microbial inoculum.

[0034] Inoculate Bacillus licheniformis (Bacillus licheniformis) with preservation number CGMCCNo.11235 into LB medium (containing 10g / L of tryptone, 5g / L of yeast extract and 10g / L of NaCl), at 30°C and 200 rpm Cultivate with shaking / min. The bacterium liquid of gained is the microbial inoculum of the present embodiment, and in every gram of this microbial inoculum, the number of live bacteria is 10 9 cfu.

Embodiment 2

[0036] This example is used to illustrate the cultivation of Bacillus licheniformis of the present invention and the preparation of microbial inoculum.

[0037] Inoculate Bacillus licheniformis (Bacillus licheniformis) with preservation number CGMCCNo.11235 into beef extract peptone medium (containing 20g / L of peptone, 5g / L of beef extract and 5g / L of NaCl), at 37°C and 180 rpm Cultivate under shaking / min to obtain bacterial liquid. Gained bacterium liquid is through aseptic filtration, promptly obtains the microbial inoculum agent of the present embodiment after freeze-drying, and in every gram of this microbial inoculum agent, the number of live bacteria is 10 10 cfu.

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Abstract

The invention provides Bacillus licheniformis, collected under CGMCC No.11235.The invention also provides a microbial agent comprising the Bacillus licheniformis described herein as an effective ingredient.The invention also provides the application of the Bacillus licheniformis described herein and the microbial agent described herein in fermentation bed culture.Through the above-mentioned technical scheme, it is possible to more effectively remove stink of livestock and poultry.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a bacillus licheniformis, a microbial agent and their application in fermentation bed cultivation. Background technique [0002] In recent years, my country's poultry breeding industry has developed rapidly, and gradually tends to be large-scale and intensive. The waste water, waste gas, and garbage discharged from the farms have caused serious pollution to the air, soil, and water in the surrounding areas. Among them, the malodorous gas from the livestock farms has the most significant impact on the senses and health of the livestock and the surrounding population. The composition of malodorous gas in livestock and poultry farms is complex. The traditional method of dealing with malodorous gas is to clean frequently, ventilate more, and flush with water. This method wastes a lot of water resources, and at the same time increases the humidity in the farm, which can easily...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09B3/00A61L9/01C12R1/10
Inventor 张丽耿兵朱昌雄田云龙叶婧郭萍李红娜刘雪李峰
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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